Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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ACE Inhibitory and Antioxidative Activities of Silkworm Larvae (Bombyx mori) Hydrolysate

번데기 가수분해물의 ACE 저해활성과 항산화활성

  • Yu, Jung-Sik (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Woo, Koan-Sik (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Youn-Ri (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kang, Tae-Su (Dept. of Biofood Science and Biotechnology, Chungbuk Provincial College of Science and Technology) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 유정식 (충북대학교 식품공학과) ;
  • 우관식 (충북대학교 식품공학과) ;
  • 황인국 (충북대학교 식품공학과) ;
  • 이연리 (충북대학교 식품공학과) ;
  • 강태수 (충북과학대학 바이오식품생명과학과) ;
  • 정헌상 (충북대학교 식품공학과)
  • Published : 2008.02.29
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Abstract

In order to utilize the silkworm larvae (Bombyx mori) protein, defatted silkworm protein was hydrolysed by four enzymes (pepsin, trypsin, neutrase and alcalase) at various hydrolysis times (6, 12, 18, 24 and 30 hr) and suspension concentrations (2, 5, 10, 15 and 20%). Protein solubility index, ACE (angiotensin converting enzyme) inhibitory activity and antioxidative activity of silkworm protein hydrolysates were investigated. The optimum condition of hydrolysis was 10% suspension concentration and 18 hr. Protein solubility index of trypsin treatment was higher than other enzyme treatments. ACE inhibitory activity and $IC_{50}$ value of antioxidative activity of neutrase treatment were 86.16% at $100\;{\mu}g/mL$ and $352.75\;{\mu}g/mL$, respectively; also, these values were higher than other enzyme treatments.

번데기 단백질의 최적 가수분해 조건을 선정하기 위해 4종의 가수분해 효소를 이용하여 번데기분말 첨가량별 및 가수분해시간에 따른 단백질 용해지수, 각각의 가수분해물의 ACE 저해활성 및 항산화활성 등을 조사하였다. 탈지번데기 분말의 최적 분산농도는 $5{\sim}10%$ 범위였으며 가수분해시간은 18시간이었고, ACE 저해활성은 neutrase, trypsin, pepsin 및 alcalase 효소처리 시 각각, 85.16, 83.46, 70.45 및 65.39%로 모든 효소 처리구가 비효소 처리구(24.94%)에 비해 높은 활성을 나타내었다. 가수분해물의 항산화활성($IC_{50}$)은 상업용 효소인 neutrase와 alcalase의 경우 각각 352.75 및 $396.09\;{\mu}g/mL$로 비효소처리구($327.24\;{\mu}g/mL$)와 유사한 값을 보였고, pepsin 및 trypsin은 각각 626.09 및 $677.44\;{\mu}g/mL$로 비효소처리구보다 낮게 나타났다.

Keywords

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