BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Downregulation of matrix metalloproteinases in hyperplastic dental follicles results in abnormal tooth eruption

  • Kim, Seong-Gon (Dept. of Oral and Maxillofacial Surgery, Hallym University) ;
  • Kim, Myung-Hee (Dept. of Orthodontics, Kaya Dental Hospital) ;
  • Chae, Chang-Hoon (Forgene Inc.) ;
  • Jung, Youn-Kwan (Dept. of Biochemistry & Cell Biology, Skeletal Diseases Genome Research Center, Kyungpook National University) ;
  • Choi, Je-Yong (Dept. of Biochemistry & Cell Biology, Skeletal Diseases Genome Research Center, Kyungpook National University)
  • 투고 : 2007.10.16
  • 심사 : 2007.12.19
  • 발행 : 2008.04.30
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초록

In this study, we compared the gene expression profiles of non-syndromic hyperplastic dental follicle (HDF) fibroblasts and normal dental follicle (NDF) fibroblasts using cDNA micro-arrays, quantitative PCR, and immunohistochemical staining. Microarray analysis showed that several collagens genes were upregulated in the HDF's, including collagen types I, IV, VIII, and XI and TIMP-1, -3, and -4 (fold ratio > 2.0). In contrast, the expression of MMP-1, -3, -10, and -16 together with IL-8 was more than two fold downregulated. The differential expression of the genes encoding alkaline phosphatase, MMP-1, -3, -8, and IL-8 was confirmed by quantitative RT-PCR, while that of 24 HDFs and 18 NDFs was confirmed by immunohistochemical analysis. However, HDFs showed stronger expression of MMP-3 than NDFs (P < 0.001). Collectively, these results indicate that defective regulation of MMPs mediating connective tissue remodeling may be responsible for abnormal tooth eruption.

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참고문헌

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