Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Development and sustained release effect of implantable formula of somatotropin

Somatotropin 이식 제형 개발과 지속적 방출 효과

  • Accepted : 2008.03.10
  • Published : 2008.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was carried out to develop a sustained release implantable formula of bovine somatotropin (SRIF-BST) and to examine its sustained release effect. The SRIF-BST was produced by coating a solid pellet, which was comprised of BST and an excipient, made of a biodegradable polymer and poloxamer, which are capable of regulating the rate of BST release. The coated membrane of SRIFBST was observed with a field emission scanning electron microscope. The thickness of the coated membrane was approximately $1{\mu}m$, and the pore sizes of the coated membrane surface were below $10{\mu}m$. In dissolution test, the release duration of the SRIF-BST maintained for 10 days, whereas the release duration of the control BST formula maintained for 3 days. In weight gain assay and tibia test of hypophysectomized rats, the release duration of the SRIF-BST treated group was 12 days and the net weight gain was 53.16 g, also the tibia length and strength of the SRIF-BST treated group was increased 10.5% and 23.1% compared with those of the control group, respectively.

Keywords

References

  1. Azain MJ, Kasser TR, Sabacky MJ, Baile CA. Comparison of the growth-promoting properties of daily versus continuous administration of somatotropin in female rats with intact pituitaries. J Anim Sci 1993, 71, 384-392 https://doi.org/10.2527/1993.712384x
  2. Azain MJ, Roberts TJ, Martin RJ, Kasser TR. Comparison of daily versus continuous administration of somatotropin on growth rate, feed intake, and body composition in intact female rats. J Anim Sci 1995, 73, 1019-1029
  3. Bellini MH, Bartolini P. In vivo bioassay for the potency determination of human growth hormone in dwarf 'little' mice. Endocrinology 1993, 132, 2051-2055 https://doi.org/10.1210/en.132.5.2051
  4. Collier RJ, Byatt JC, Denham SC, Eppard PJ, Fabellar AC, Hintz RL, McGrath MF, McLaughlin CL, Shearer JK, Veenhuizen JJ, Vicini JL. Effects of sustained release bovine somatotropin (sometribove) on animal health in commercial dairy herds. J Dairy Sci 2001, 84, 1098-1108 https://doi.org/10.3168/jds.S0022-0302(01)74570-5
  5. Etherton TD, Bauman DE. Biology of somatotropin in growth and lactation of domestic animals. Physiol Rev 1998, 78, 745-761 https://doi.org/10.1152/physrev.1998.78.3.745
  6. Gallo L, Bailoni L, Schiavon S, Carnier P, Ramanzin M, Andrighetto I, Bittante G. Effect of slow-release somatotropin on the pattern of milk yield between and within injection intervals. J Dairy Sci 1997, 80, 46-51 https://doi.org/10.3168/jds.S0022-0302(97)75911-3
  7. Gallo L, Cassandro M, Carnier P, Mantovani R, Ramanzin M, Bittante G, Tealdo E, Casson P. Modeling response to slow-releasing somatotropin administered at 3- or 4-week intervals. J Dairy Sci 1994, 77, 759-769 https://doi.org/10.3168/jds.S0022-0302(94)77010-7
  8. Groesbeck MD, Parlow AF. Highly improved precision of the hypophysectomized female rat body weight gain bioassay for growth hormone by increased frequency of injections, avoidance of antibody formation, and other simple modifications. Endocrinology 1987, 120, 2582-2590 https://doi.org/10.1210/endo-120-6-2582
  9. Harn NR, Jeng YN, Kostelc JG, Middaugh CR. Spectroscopic analysis of highly concentrated suspensions of bovine somatotropin in sesame oil. J Pharm Sci 2005, 94, 2487-2495 https://doi.org/10.1002/jps.20464
  10. Hartnell GF, Franson SE, Bauman DE, Head HH, Huber JT, Lamb RC, Madsen KS, Cole WJ, Hintz RL. Evaluation of sometribove in a prolonged-release system in lactating dairy cows-production responses. J Dairy Sci 1991, 74, 2645-2663 https://doi.org/10.3168/jds.S0022-0302(91)78443-9
  11. Helling HJ, Prokop A, Schmid HU, Nagel M, Lilienthal J, Rehm KE. Biodegradable implants versus standard metal fixation for displaced radial head fractures. A prospective, randomized, multicenter study. J Shoulder Elbow Surg 2006, 15, 479-485 https://doi.org/10.1016/j.jse.2005.10.010
  12. Jenny BF, Grimes LW, Pardue FE, Rock DW, Patterson DL. Lactational response of Jersey cows to bovine somatotropin administered daily or in a sustained-release formulation. J Dairy Sci 1992, 75, 3402-3407 https://doi.org/10.3168/jds.S0022-0302(92)78116-8
  13. Kajkowski EM, Price LA, Pausch MH, Young KH, Ozenberger BA. Investigation of growth hormone releasing hormone receptor structure and activity using yeast expression technologies. J Recept Signal Transduct Res 1997, 17, 293-303 https://doi.org/10.3109/10799899709036610
  14. Klindt J, Buonomo FC, Yen JT. Administration of porcine somatotropin by sustained-release implant: growth, carcass, and sensory responses in crossbred white and genetically lean and obese boars and gilts. J Anim Sci. 1995, 73, 1327-1339
  15. McLaren DG, Bechtel PJ, Grebner GL, Novakofski J, McKeith FK, Jones RW, Dalrymple RH, Easter RA. Dose response in growth of pigs injected daily with porcine somatotropin from 57 to 103 kilograms. J Anim Sci 1990, 68, 640-651 https://doi.org/10.2527/1990.683640x
  16. Pineda LM, Busing M, Meinig RP, Gogolewski S. Bone regeneration with resorbable polymeric membranes. III. Effect of poly(L-lactide) membrane pore size on the bone healing process in large defects. J Biomed Mater Res 1996, 31, 385-394 https://doi.org/10.1002/(SICI)1097-4636(199607)31:3<385::AID-JBM13>3.0.CO;2-I
  17. Roberts TJ, Azain MJ. Effect of somatotropin on rate and composition of gain and feed intake in young, pituitary intact female rats. Growth Dev Aging 1995, 59, 3-13
  18. Sadaf S, Khan MA, Akhtar MW. Production of bubaline somatotropin by auto-induction in Escherichia coli. Biotechnol Appl Biochem 2007, 47, 21-26 https://doi.org/10.1042/BA20060154
  19. Trepod CM, Mott JE. A spontaneous runaway vector for production-scale expression of bovine somatotropin from Escherichia coi. Appl Microbiol Biotechnol 2002, 58, 84-88 https://doi.org/10.1007/s00253-001-0859-6
  20. Thiel LF, Beermann DH, Krick BJ, Boyd RD. Dosedependent effects of exogenous porcine somatotropin on the yield, distribution, and proximate composition of carcass tissues in growing pigs. J Anim Sci 1993, 71, 827-835 https://doi.org/10.2527/1993.714827x
  21. Warren WC, Bentle KA, Schlittler MR, Schwane AC, O'Neil JP, Bogosian G. Increased production of peptide deformylase eliminates retention of formylmethionine in bovine somatotropin overproduced in Escherichia coli. Gene 1996, 174, 235-238 https://doi.org/10.1016/0378-1119(96)00086-8
  22. Yen JT, Klindt J, Kerr BJ, Buonomo FC. Lysine requirement of finishing pigs administered porcine somatotropin by sustained-release implant. J Anim Sci 2005, 83, 2789-2797