Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Analgesic effect of Clematidis Radix (CR) herb-acupuncture in a rat model of pain and inflammation

  • Hwang, Hye-Jeong (Acupuncture & Meridian Science Research Center, Kyung-Hee University) ;
  • Kim, Seung-Tae (Acupuncture & Meridian Science Research Center, Kyung-Hee University) ;
  • Lee, Hye-Jung (Acupuncture & Meridian Science Research Center, Kyung-Hee University) ;
  • Kim, Young-Suk (Department of Oriental Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Shim, In-Sop (Department of Integrative Medicine, The Catholic University of Korea) ;
  • Park, Hi-Joon (Acupuncture & Meridian Science Research Center, Kyung-Hee University) ;
  • Choi, Won-Ju (Department of Oriental Medicine, Dongkuk University) ;
  • Kim, Jang-Hyun (Department of Oriental Medicine, Dongkuk University) ;
  • Hahm, Dae-Hyun (Acupuncture & Meridian Science Research Center, Kyung-Hee University)
  • Published : 2008.02.29
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Abstract

Anti-nociceptive and anti-inflammatory effects of Clematidis Radix (CR)-distillates were investigated in three different pain animal models. Highly purified distillate of CR was injected to Zusanli (ST36) acupoint, called CR herb-acupuncture in the Korean traditional medicine. In tail flick latency test, the CR herb-acupuncture treatment did not show a significant effect of relieving acute phasic pain. To investigate the anti-inflammatory effect of CR herb-acupuncture, inflammatory pain was induced by subcutaneous injection of formalin to the plantar tissue or intra-articular injection of carrageenan to the tibio-tarsal joint in the rats. And the medicinal effect of CR herb-acupuncture was evaluated by analyzing pain behavior such as licking or biting behavior, or by measuring weight distribution ratio between two foot and ankle circumference. In the rat formalin test, the analgesic effect of CR herb-acupuncture was more pronounced in the late phase (for 20 min after the early phase) than in the early phase (for the first 10 min post formalin injection). It also significantly alleviated the carrageenan-induced monoarthritis, in terms of weight distribution ratio and ankle edema. These results revealed that CR herb-acupuncture was effective to treat the inflammatory pain and could be used as an analgesic treatment with an antiinflammatory effect.

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References

  1. Ahn DK. (1998) Illustrated Book of Korean Medical Herbs, p. 304, Kyo-Hak publishing, Seoul
  2. Bennell K, Hinman R. (2005) Exercise as a treatment for osteoarthritis. Curr. Opin. Rheumatol. 17, 634-640 https://doi.org/10.1097/01.bor.0000171214.49876.38
  3. Berman BM, Singh BB, Lao L, Langenberg P, Li H, Hadhazy V, Bareta J, Hochberg M. (1999) A randomized trial of acupuncture as an adjunctive therapy in osteoarthritis of the knee. Rheumatology 38, 346-354 https://doi.org/10.1093/rheumatology/38.4.346
  4. Fang JQ, Liu F, Shao XM, Wu YY. (2007) Effect of electroacupuncture on carrageenan-induced inflammation, IL-1beta and TNF-alpha concentrations and their mRNA expressions in toe tissue in rats. Zhen Ci Yan Jiu 32, 224-228
  5. Filshie J, White A. (1998) Medical Acupuncture-A Western Scientific Approach. 1st ed., pp. 341-360, Churchill Livingstone, London
  6. Handy RL, Moore PK. (1998) A comparison of the effects of L-NAME, 7- NI and L-NIL on carrageenaninduced hindpaw edema and NOS activity. Br. J. Pharmacol. 123, 1119-1126 https://doi.org/10.1038/sj.bjp.0701735
  7. Holsapple MP, Schnur M, Yim GK. (1980) Pharmacological modulation of edema mediated by prostaglandin, serotonin and histamine. Agents Actions 10, 368-373 https://doi.org/10.1007/BF01971442
  8. Hunskaar S, Berge OG, Hole K. (1986) Dissociation between antinociceptive and anti-inflammatory effects of acetylsalicylic acid and indomethacin in the formalin test. Pain 25, 125-132 https://doi.org/10.1016/0304-3959(86)90014-X
  9. Hunskaar S, Hole K. (1987) The formalin test in mice: dissociation between inflammatory and noninflammatory pain. Pain 30, 103-114 https://doi.org/10.1016/0304-3959(87)90088-1
  10. Kim HW, Kwon YB, Han HJ, Yang IS, Beitz AJ, Lee JH. (2005) Antinociceptive mechanisms associated with diluted bee venom acupuncture (apipuncture) in the rat formalin test: involvement of descending adrenergic and serotonergic pathways. Pharmacol. Res. 51, 183-188 https://doi.org/10.1016/j.phrs.2004.07.011
  11. Kwon YB, Lee JD, Lee HJ, Han HJ, Mar WC, Kang SK, Beitz AJ, Lee JH. (2001) Bee venom injection into an acupuncture point reduces arthritis associated edema and nociceptive responses. Pain 90, 271-280 https://doi.org/10.1016/S0304-3959(00)00412-7
  12. Lee JD, Kim SY, Kim TW, Lee SH, Yang HI, Lee DI, Lee YH. (2004) Anti-inflammatory effect of bee venom on type II collagen-induced arthritis. Am. J. Chin. Med. 32, 361-367 https://doi.org/10.1142/S0192415X04002016
  13. Lee SK, Lee JD, Koh HK, Park DS, Lee YH, Kang SK. (2000) The study on the Hominis Placenta Aquaacupuncture solution. J. Korean Acupuncture Moxi. Soc. 17, 67-74
  14. Li RW, David Lin G, Myers SP, Leach DN. (2003) Anti-inflammatory activity of Chinese medicinal vine plants. J. Ethnopharmacol. 85, 61-67 https://doi.org/10.1016/S0378-8741(02)00339-2
  15. Nikfar S, Abdollahi M, Etemad F, Sharifzadeh M. (1997) Effects of sweetening agents on morphineinduced analgesia in mice by formalin test. Gen. Pharmacol. 29, 583-586 https://doi.org/10.1016/S0306-3623(96)00575-7
  16. Nikfar S, Abdollahi M, Sharifzadeh M, Eftekhar N. (1998) Interaction between lead acetate and morphine on antinociception in mice by formalin test. Gen. Pharmacol. 30, 489-493 https://doi.org/10.1016/S0306-3623(97)00283-8
  17. Rosland JH, Tjoisen A, Maehle B, Hole K. (1990) The formalin test in mice, effect of formalin concentration. Pain 42, 235-242 https://doi.org/10.1016/0304-3959(90)91167-H
  18. Ross J. (1995) Acupuncture Point Cominations: the Key to Clinical Success. 1st ed., Churchill Livingstone, Edinburgh
  19. Schott E, Berge OG, Angeby-Moller K, Hammarstorm G, Dalsgaard CJ, Brodin E. (1994) Weight bearing as an objective measure of arthritic pain in the rat. J. Pharmacol. Toxicol. Methods 31, 79-83 https://doi.org/10.1016/1056-8719(94)90046-9
  20. Shibata M, Ohkubo T, Takahashi H, Inoki R. (1989) Modified formalin test: characteristic biphasic pain response. Pain 38, 347-352 https://doi.org/10.1016/0304-3959(89)90222-4
  21. Stochla K, Maslinski S. (1982) Carrageenan-induced oedema in the rat paw.-histamine participation. Agents Actions 12, 201-202 https://doi.org/10.1007/BF01965145
  22. Stux G, Berman B, Pomeranz B, Kofen P. (1995) Basics of acupuncture. 3rd ed., pp. 1-250, Springer-Verlag, Berlin
  23. Urban MO, Gebhart GF. (1999) Supraspinal contributions to hyperalgesia. Proc. Natl. Acad. Sci. USA. 96, 7687-7692 https://doi.org/10.1073/pnas.96.14.7687
  24. Wirth KJ, Alpermann HG, Satoh R, Inazu M. (1992) The bradykinin antagonist Hoe 140 inhibits carrageenan- and thermically induced paw oedema in rats. Agents Actions Suppl. 38, 428-431
  25. Yeom MJ, Lee HC, Kim GH, Shim I, Lee HJ, Hahm DH. (2003) Therapeutic effects of Hominis placenta injection into an acupuncture point on the inflammatory responses in subchondral bone region of adjuvantinduced polyarthritic rat. Biol. Pharm. Bull. 26, 1472-1477 https://doi.org/10.1248/bpb.26.1472
  26. Zhang Y, Shaffer A, Portanova J, Seibert K, Isakson PC. (1997) Inhibition of cyclooxygenase-2 rapidly reverses inflammatory hyperalgesia and prostaglandin E2 production. J. Pharmacol. Exp. Ther. 283, 1069-1075