Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Characterization of Bacteria and Their Antibiotic Sensitivities in Porcine Liquid Semen

돼지 액상정액 내 세균오염과 항생제 감수성에 관한 연구

  • 유재원 (농촌진흥청 국립축산과학원) ;
  • 조규호 (농촌진흥청 국립축산과학원) ;
  • 홍준기 (농촌진흥청 국립축산과학원) ;
  • 김명직 (농촌진흥청 국립축산과학원) ;
  • 박준철 (농촌진흥청 국립축산과학원) ;
  • 정일병 (농촌진흥청 국립축산과학원) ;
  • 김인철 (농촌진흥청 국립축산과학원)
  • Published : 2008.12.31
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Abstract

The semen collection process in the porcine is far from being a sterile procedure. Consequently, porcine ejaculates commonly contain bacterial contaminants. The aim of this study is to identify the bacteria in porcine semen and to find the antibiotics resistance of bacteria. Twelve porcine originating from four AI center were used to collect semen. Bacteria were identified by automated instrument for rapid organism identification system and bacterial sensitivities of 8 antibiotics were tested. The Bacterial contaminants of Staphylococcus genus(37.8%), Proteus genus(7.0%), Bacillus genus (6.1%), Pasteulla genus(5.7%), Acinetobacte genus(5.2%), Serratia genus(4.3%) and others(33.9%) were frequently isolated. However, amikacin showed higher antibiotic sensitivity than other antibiotics. General sanitation protocols can contribute partly to inhibit the bacterial contamination, with monitoring boar housing, semen collection areas and the extended semen. But, proper selection of preservative antibiotics by microbial sensitivities can minimize the influence of bacteria.

정액 내 세균오염 종류와 검출 빈도를 조사한 결과는 Table 1에서 보는 바와 같다. 돼지 정액 내 오염세균 중 검출빈도는 Staphylo- coccus genus, Proteus genus, Bacillus genus, Pasteulla genus, Acinetobacte genus 및 Serratia genus 등의 순으로 나타났다. 동정된 세균 중 33종의 세균에 대하여 디스크법을 이용하여 8종의 항생제에 대한 감수성을 조사 한 결과는 Table 2와 3에서 보는 바와 같다. 검출된 세균의 항생제 감수성을 조사한 결과 항생제 감수성이 높은 항생제는 Amikacin, Polymyxin B, Neomycin, Streptomycin, Kanamycin, Tetracycline, Erythromycin 및 Penicillin순 이었다. Enterococcus faecium와 Streptococcus uberis는 Streptomycin을 제외한 모든 항생제에 대하여 저항성이 있는 것으로 조사되었다.

Keywords

References

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