The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Influence of Five Herbal Medicines on Cytochrome P450 3A4 Drug-Metabolizing Enzymes in Human Liver Microsomes

수종 지혈 한약물이 Cytochrome P450 3A4 활성에 미치는 영향

  • Hwang, Jin-Woo (Department of Oriental internal Medicine, National Medical Center) ;
  • Go, Jae-Eon (Department of internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Go, Ho-Yeon (Department of internal Medicine, College of Oriental Medicine, Semyung University) ;
  • Choi, You-Kyung (Department of internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Park, Jong-Hyung (Department of internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Jun, Chan-Yong (Department of internal Medicine, College of Oriental Medicine, Kyungwon University)
  • 황진우 (국립의료원 한방진료부 한방내과) ;
  • 고재언 (경원대학교 한의과대학 내과학교실) ;
  • 고호연 (세명대학교 한의과대학 내과학교실) ;
  • 최유경 (경원대학교 한의과대학 내과학교실) ;
  • 박종형 (경원대학교 한의과대학 내과학교실) ;
  • 전찬용 (경원대학교 한의과대학 내과학교실)
  • Published : 2008.12.31
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Abstract

Objects : The aim of this study was to investigate the influence of five herbal medicines on cytochrome P450 3A4 drug-metabolizing enzymes in human liver microsomes. Methods : To use human liver microsomes, an extract of five herbal medicines, which are Artemisia princeps Pampan, Sophora jeponica Linne, Panax notoginseng F. H. Chen, Lithospermum Erythrorhizon Sieb., and Cirsium maackii Maxim, which together are called Jihyulyak(止血藥, drugs for arresting bleeding, hemostatics), was co-incubated and measured for relative enzyme activity in incubation condition compared to ketoconazole, a representative inhibitor of CYP 3A4. Results : We showed that all five of the traditional herbal medicines had no inhibition effect of CYP 3A4 at 10, 20, 30, 40, and $50{\mu}g/ml$ doses in human liver microsomes, although Sophora japonica Linne(SJL) showed a little inhibition at about 81% inhibition rate of control. However, this result is not enough to prove that SJL has a CYP 3A4 inhibition effect. Moreover, we can't make sure that those rates had significant induction effect on CYP 3A4. Conclusions : The result of this study could support that those herbal medicines are safer than chemical drugs, even if this is the basic step to prove that result.

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