Optimal Conditions for Propagation in Bottom and Top Brewing Yeast Strains

  • Cheong, Chul (Department of Fermented Food Science, Seoul University of Venture & Information) ;
  • Wackerbauer, Karl (Department of Biotechnology, Technical University of Berlin) ;
  • Lee, Si-Kyung (Department of Applied Biological Science, Konkuk University) ;
  • Kang, Soon-Ah (Department of Fermented Food Science, Seoul University of Venture & Information)
  • 발행 : 2008.08.31

초록

The method of yeast propagation has an influence on yeast physiology, fermentation ability, flocculation rate, and taste stability of beer. In order to find optimal conditions for propagation, several parameters were investigated in combinations. The bottom brewing yeast grown at $10^{\circ}C$ indicated that a higher flocculation capacity during the $1^{st}$ fermentation. However, the taste stability and the aroma profile were not affected by parameters of propagation investigated. The beer quality was rather affected by storage duration. In addition, a correlation between tasting and chemiluminescence was found at the beer, which was produced using bottom brewing yeast. The propagation at $10-25^{\circ}C$ with addition of zinc ion indicated the best condition to improve fermentation ability, flocculation rate, and filterability for bottom brewing yeast, whereas the propagation at $30^{\circ}C$ with addition of zinc ion showed the best condition to increase fermentation ability for top brewing yeasts.

키워드

참고문헌

  1. Wackerbauer K, Tayama T, Fitzner M, Kunerth S. Proper management of pitching yeast. Brauwelt 3: 80-87 (1997)
  2. Martin V, Quain DE, Smart KA. The oxidative stress response of ale and lager yeast strains. EBC Congress 79: 679-686 (1999)
  3. Thurston PA, Taylor R, Ahvenainen J. Effects of linoleic acid supplements on the synthesis by yeast of lipids and acetate esters. J. Inst. Brew. 87: 92-95 (1981) https://doi.org/10.1002/j.2050-0416.1981.tb03995.x
  4. Ahvenainen J. Lipid composition of aerobically and anaerobically propagated brewer's bottom yeast. J. Inst. Brew. 88: 367-370 (1982) https://doi.org/10.1002/j.2050-0416.1982.tb04123.x
  5. Cho WI, Choi JB, Lee KP, Cho SC, Park EJ, Chung MS, Pyun YR. Antimicrobial activity of medical plants against Bacillus subtilis spore. Food Sci. Biotechnol. 16: 1072-1077 (2007)
  6. Lee EJ, Kim SG, Yoo SR, Oh SS, Hwang IG, Kwon GS, Park JK. Microbial cantamination by Bacillus cereus, Clostridium perfringens, and Enterobacter sakazakii in sunsik. Food Sci. Biotechnol. 16: 948-953 (2007)
  7. Van Iersel MFM, Meersman E, Arntz M, Rombouts FM, Abee T.Effect of environmental conditions on flocculation and immobilization of brewer's yeast during production of alcohol free beer. J. Inst. Brew. 104: 131-136 (1998) https://doi.org/10.1002/j.2050-0416.1998.tb00986.x
  8. Alex speers R, Tung MA, Durance TD, Stewart GG. Biochemical aspects of yeast flocculation and its measurement: A review. J. Inst. Brew. 98: 293-300 (1992) https://doi.org/10.1002/j.2050-0416.1992.tb01111.x
  9. Van Hamersveld EH, Van der Lans RGJM, Caulet PJC, Luyben KChAM. Modeling brewer's yeast flocculation. Biotechnol. Bioeng. 57: 331-341 (1998)
  10. Kringstad H, Rasch S. The influence of the method of preparation of pitching yeast on its production of diacetyl and acetoin during fermentation. J. Inst. Brew. 72: 56-61 (1996)
  11. Pascale B, Dengis D, Rouxhet G. Flocculation mechanisms of top and bottom fermentation brewing yeast. J. Inst. Brew. 103: 257-261 (1997) https://doi.org/10.1002/j.2050-0416.1997.tb00954.x
  12. Van der Aar PC, Strava MH, Teunssen WRH. Flocculation of brewer's lager yeast. EBC Congress 73: 259-266 (1993)
  13. Cheong C, Wackerbauer K, Kang SA. Influence of aeration during propagation of pitching yeast on fermentation and beer flavour. J. Microbiol. Biotechn. 17: 297-304 (2007)
  14. Donhauser S, Wagner D. Possible effect of zinc contents in wort. Monatsschr. Brauwiss. 6: 223-230 (1986)
  15. Brown CM, Johnson B. Influence of oxygen tension on the physiology of Saccharomyces cerevisiae in continuous culture. Anton Leeuw. Int. J. G. 37: 477-487 (1971) https://doi.org/10.1007/BF02218518
  16. Kruger L, Pickerell TW, Axcell B. The sensitivity of different brewing yeast to carbon dioxide inhibition: Fermentation and production of flavour-active volitile compounds. J. Inst. Brew. 98: 133-138 (1992) https://doi.org/10.1002/j.2050-0416.1992.tb01100.x
  17. Knatchbull FB. The effect of low $CO_2$ pressures on the absorption of amino acids and production of flavour-active volitiles by yeast. J. Inst. Brew. 93: 420-424 (1987) https://doi.org/10.1002/j.2050-0416.1987.tb04530.x
  18. Collinson LP, Dawes IW. Inducibility of the response of yeast cells to peroxide stress. J. Gen. Microbiol. 138: 329-335 (1992) https://doi.org/10.1099/00221287-138-2-329
  19. Lehmann J, Back W. Practical change of yeast assimilation. Brauindustrie 4: 225-228 (1997)
  20. Pfenninger H. Brewing Technical Analysis Method II. 3rd ed. Freising, Germany. pp. 203-207 (1993)
  21. Wackerbauer K, Zufall C, Legand J. Discover of taste instability of beer. Brauwelt 40/41: 1796-1803 (1999)
  22. Klein H, Krammer R, Natter M. Determination for predict of taste stability of beer. EBC Congress 77: 553-560 (1997)
  23. Maemura H, Morimura S, Kida K. Effects of aeration during the cultivation of pitching yeast on its characteristic during the subsequent fermentation on wort. J. Inst. Brew. 104: 207-211 (1998) https://doi.org/10.1002/j.2050-0416.1998.tb00993.x
  24. Van Hamersveld EH, Van Loosdrecht MCM, Van der Lans RGJM, Luyben KChAM. On the measurement of the flocculation characteristics of brewer's yeast. J. Inst. Brew. 102: 333-342 (1996) https://doi.org/10.1002/j.2050-0416.1996.tb00919.x
  25. Garoux G, Haubursin H, Bilbault S, Dufour JP. Yeast flocculation: Biochemical characterization of yeast cell wall components. EBC Congress 73: 275-281 (1993)
  26. Kang MH, Saraswat JL, Park YH. Production of lyophilizied culture of Lactobacillus acidophilus with preserving cell viability. J. Microbiol. Biotechn. 11: 1055-1060 (1990)
  27. Park JE, Lee KH, Jahang DJ. Effect of trehalose on bioluminescence and viability of freeze-dried baterial cells. J. Microbiol. Biotechn. 12: 349-353 (2002)