Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Multiplex PCR Detection of the MON1445, MON15985, MON88913, and LLcotton25 Varieties of GM Cotton

  • Kim, Jae-Hwan (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Sun-A (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Seo, Young-Ju (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Woo-Young (BioFood Team, Korea Food & Drug Administration) ;
  • Park, Sun-Hee (BioFood Team, Korea Food & Drug Administration) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2008.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 4 varieties of genetically modified (GM) cotton. The event-specific primers were used to distinguish the 4 varieties of GM cotton (MON1445, MON15985, MON88913, and LLcotton25) using multiplex PCR. The acyl carrier protein 1 (Acp1) gene was used as an endogenous reference gene of cotton in the PCR detection. The primer pair Acp1-AF/AR containing a 99 bp amplicon was used to amplify the Acp1 gene and no amplified product was observed in any of the 13 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM cotton containing MON1445, MON15985, MON88913, and LLcotton25.

Keywords

References

  1. James C. Global Status of Commercialized Biotech/GM Crops: 2007. ISAAA Briefs No. 37. International Service for the Acquisition of Agri-biotech Applications, Ithaca, NY, USA (2007)
  2. Agbios database (http://www.agbios.com). Accessed Dec. 1, 2007
  3. Kim JH, Jee SM, Park CS, Kim HY. Detection of transgenic rice containing cry1Ac gene derived from Bacillus thuringiensis by PCR. Food Sci. Biotechnol. 15: 625-630 (2006)
  4. Miraglia M, Berdal KG, Brera C, Corbisier P, Holst-Jensen A, Kok EJ, Marvin HJP, Schimmel H, Rentsch J, van Rie JPPF, Zagon J. Detection an d traceability of genetically modified organisms in the food production chain. Food Chem. Toxicol. 42: 1157-1180 (2004) https://doi.org/10.1016/j.fct.2004.02.018
  5. Duijn G, Biert R, Bleeker-Marcelis H, Boeijen I, Adan AJ, Jhakrie S, Hessing M. Detection of genetically modified organisms in foods by protein- and DNA-based techniques: Bridging the methods. J. Assoc. Off. Ana. Chem. Int. 85: 787-791 (2002)
  6. Heo MS, Kim JH, Shin WS, Park SH, Park HK, Kim MC. Limit of detection for genetically modified soybean in doenjang (Korean fermented soypaste). Food Sci. Biotechnol. 13: 657-661 (2004)
  7. Shin DW, Park SH, Woo GJ, Kim HY, Park CS. Case study for natural gene transfer from genetically modified food to food microorganisms. Food Sci. Biotechnol. 13: 342-346 (2004)
  8. Holst-Jensen A, Ronning SB, Lovseth A. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 375: 985-993 (2003) https://doi.org/10.1007/s00216-003-1767-7
  9. Kim JH, Song HS, Heo MS, Lee WY, Lee SH, Park SH, Park HK, Kim MC, Kim HY. Detection of eight different events of genetically modified maize by multiplex PCR method. Food Sci. Biotechnol. 15: 148-151 (2006)
  10. Heo MS, Kim JH, Park SH, Woo GJ, Kim HY. Detection of genetically modified maize by multiplex PCR method. J. Microbiol. Biotechn. 14: 1227-1231 (2004)
  11. Hernandez M, Rodriguez-Lazaro D, Zhang D, Esteve T, Pla M, Prat S. Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize events: Bt11, MON810, T25, and GA21. J. Agr. Food Chem. 53: 3333-3337 (2005) https://doi.org/10.1021/jf049192y
  12. Demeke T, Giroux RW, Reitmeier S, Simon SL. Development of a polymerase chain reaction assay for detection of three canola transgenes. J. Am. Oil Chem. Soc. 79: 1015-1019 (2002) https://doi.org/10.1007/s11746-002-0595-2
  13. Kim JH, Kim TW, Lee WY, Park SH, Kim HY. Multiplex PCR detection of the GT73, MS8xRF3, and T45 varieties of GM canola. Food Sci. Biotechnol. 16: 104-109 (2007)
  14. Germini A, Zanetti A, Salati C, Rossi S, Forre C, Schmid S, Fogher C, Marchelli R. Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J. Agr. Food Chem. 52: 4350-4350 (2004)
  15. James D, Schmidt AM, Wall E, Green M, Masri S. Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis. J. Agr. Food Chem. 51: 5829-5834 (2003) https://doi.org/10.1021/jf0341159
  16. Yang L, Pan A, Zhang K, Yin C, Qian B, Chen J, Huang C, Zhang D. Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Transgenic Res. 14: 817-831 (2005) https://doi.org/10.1007/s11248-005-0010-z
  17. Yang L, Pan A, Zhang K, Guo J, Yin C, Chen J, Huang C, Zhang D. Identification and quantification of three genetically modified insect resistant cotton lines using conventional and TaqMan real-time polymerase chain reaction methods. J. Agr. Food Chem. 53: 6222-6229 (2005) https://doi.org/10.1021/jf050095u
  18. Lee SH, Kim JK, Yi BY. Detection methods for biotech cotton MON 15985 and MON 88913 by PCR. J. Agr. Food Chem. 55: 3351-3357 (2007) https://doi.org/10.1021/jf070036b
  19. Baeumler S, Wulff D, Tagliani L, Song P. A real-time quantitative PCR detection method specific to widestrike transgenic cotton (event 281-24-236/3006-210-23). J. Agr. Food Chem. 54: 6527-6534 (2006) https://doi.org/10.1021/jf0610357