DOI QR코드

DOI QR Code

Effect of Dietary Conjugated Linoleic Acid on Growth Performance, Carcass Characteristics and Muscular Fatty Acid Composition in Broiler

사료내 Conjugated Linoleic Acid 첨가수준이 육계의 생산성, 도체특성 및 근내 지방산 조성에 미치는 영향

  • 김영직 (대구대학교 생명자원과학부) ;
  • 김병기 (경상북도 축산기술연구소) ;
  • 윤용범 (대구대학교 생명자원과학부)
  • Published : 2008.10.31

Abstract

This study was conducted to determine the effects of dietary supplementation with conjugated linoleic acid (CLA) feeding levels (0, 0.5, 1.0, 1.5, and 2.0%) on the carcass characteristics, growth performance, serum cholesterol, and fatty acid in thigh of chicken meat. Two hundred broiler (Arbor Acre Broiler, male) were randomly assigned to five groups and were fed for five weeks and slaughtered. Thigh muscle was used for determining fatty acid composition. There was no significant difference in growth performance, such as weight gain, feed intake and feed conversion by CLA levels. Among carcass characteristics, percentage of carcass, thigh, breast, and drumstick was not influenced by the dietary CLA levels, but abdominal fat was significantly reduced with the increased CLA amount in the broilers diets (p<0.05). Higher CLA levels increased HDL-C and reduced total cholesterol and LDL-C (p<0.05). As the dietary CLA levels increased, muscular palmitic acid (saturated fatty acid) levels was increased, but the rates of oleic acid, linoleic acid, and arachidonic acid (unsaturated fatty acid) were decreased. In addition. CLA isomers were linearly increased with the increase in dietary CLA levels (p<0.05). As a conclusion, 2% of CLA feeding is possible to maximize accumulation of CLA in meat, but changes in fatty acid composition is not profitable. Therefore, 1% of CLA feeding i,j considered to be proper for accumulation of CLA and minimization of the change in fatty acid.

본 연구는 CLA를 육계사료에 첨가하여 그 급여수준(0, 0.5, 1.0, 1.5, 및 2.0%)에 따라 육계의 생산성과 CLA를 급여한 계육의 도체특성, 혈청콜레스테롤 및 지방산조성의 변화를 검토하고자 육계 200수(Arbor Acre Broiler, male)를 5주 동안 공시하였다 육계의 생산성을 나타내는 증체량, 사료섭취량 및 사료요구율은 CLA급여에 따른 유의성은 없었다. 도체특성중 도체율뿐만 아니라 도체중에 대한 간, 대퇴부위, 흉심 및 북채의 비율은 CLA를 급여함으로서 유의한 변화가 없었지만, 복강지방 비율은 CLA 급여량이 많아짐에 따라 유의한 감소 현상을 확인할 수 있었다(p<0.05). 혈청콜레스테롤은 CLA급여량이 증가할 수록 총콜레스테롤과 LDL-C은 감소하고, HDL-C은 증가하였다(p<0.05). 그리고 지방산 조성 변화는 CLA를 급여함에 따라 palmitic acid는 증가하고, oleic acid, linoleic acid 및 arachidonic acid는 감소하여 포화지방산은 증가하고 불포화지방산은 감소하는 현상을 보였다. 또한, CLA의 이성체함량도 CLA급여량이 증가함에 따라 직선적으로 증가하였다(p<0.05). 결론적으로 계육에 CLA 축적을 극대화하기 위해서는 2%까지 급여도 가능하지만. 지방산조성의 변화가 심하므로 CLA도 축적시키고 지방산 변화도 최소화할 수 있는 1%의 급여수준이 적합할 것으로 판단된다.

Keywords

References

  1. Baker, H. J., Lindsey, J. R., and Weisbroth, S. H. (1984) The laboratory rat. Academic Press Inc. NY. 2, 123-131
  2. Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L., and Pariza, M. W. (1992) Dietary sources of conjugated dieonoic isomers of linoleic acid. A newly recognized class of anticarcinogens. J. Food Comp. Anal. 5, 185-197 https://doi.org/10.1016/0889-1575(92)90037-K
  3. Corino, C., Lo Fiego, D. P., Macchioni, P., Pastorelli, G., Di Giancamillo, A., Domeneghini, C., and Rossi, R. (2007) Influence of dietary conjugated linoleic acids and vitamin E on meat quality and adipose tissue in rabbits. Meat Sci. 76, 19-28 https://doi.org/10.1016/j.meatsci.2006.10.007
  4. Du, M. and Ahn, D. U. (2002) Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poult. Sci. 81, 428-433 https://doi.org/10.1093/ps/81.3.428
  5. Du, M. and Ahn, D. U. (2003) Dietary CLA affects lipid metabolism in broiler chicks. Lipids 38, 505-511 https://doi.org/10.1007/s11745-003-1091-z
  6. Evans, K., Burdge, G. C., Wootton, S. A., Clark, M. L., and Frayn, K. N. (2002) Regulation of dietary fatty acid entrapment in subcutaneous adipose tissue and skeletal muscle. Diabetes 51, 2684-2690 https://doi.org/10.2337/diabetes.51.9.2684
  7. Folch, J., Lees, M., and Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-507
  8. Forgerty, A. C., Ford, G., and Svoronos, D. (1988) Octadeca- 9, 11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutr. Reports Int. 38, 937-934
  9. Friedewald, W. T., Levy, R. I., and Fredrickson, D. S. (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem. 18, 1163-1170
  10. Grundy, S. M. (1986) Comparison of monounsaturated fatty acid and carbohydrate for lowering plasma cholesterol. Engl. J. Med. 316, 745-751
  11. Haumann, B. F. (1996) Conjugated linoleic acid offers research promise. Inform. 7, 152-159
  12. Hood, R. L. (1984) Cellular and biochemical aspects of fat deposition in the broiler chicken. Poult. Sci. 40, 160-164 https://doi.org/10.1079/WPS19840014
  13. Houseknecht, K. L., Vanden Heuvel, J. P., Moya-Camarena, S. Y., Portocarrero, C. P., Peck, L. W., Nickel, K. P., and Belury, M. A. (1998) Dietary conjugated linoleic acid normalizes impaires glucose tolerance in the Zucker diabetic fatty of rat. Biochem. Biophys. Res. Comm. 244, 678-682 https://doi.org/10.1006/bbrc.1998.8303
  14. Hughes, P. E., Hunter, W. J., and Tove, S. B. (1982) Biohydrogenation of unsaturated fatty acids. Purification and properties of cis 9-transoctadecadienoate reductase. J. Biol. Chem. 257, 3643-3649
  15. Lauridsen, C., Mu, H., and Henckel, P. (2005) Influence of dietary conjugated linoleic acid(CLA) and age at slaughtering on performance, slaughter and meat quality, lipoproteins, and tissue deposition of CLA in barrows. Meat Sci. 69, 393-399 https://doi.org/10.1016/j.meatsci.2004.08.009
  16. Lee, K. N., Kritchevsky, D., and Pariza, M. W. (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19-25 https://doi.org/10.1016/0021-9150(94)90034-5
  17. Lee, K. N., Pariza, M. W., and Natmb, J. M. (1998) Conjugated linoleic acid decrease hepatic stearoyl-CoA desaturase mRNA expression. Biochem. Biophys. Res. Comm. 248, 817-821 https://doi.org/10.1006/bbrc.1998.8994
  18. Mossoba, M. M., McDonald, R. E., and Armstrong, D. J. (1991) Identification of minor c18 triene and conjugated diene isomers in hydrogenated soybean oil and magarine by GC-MI-FT-IR spectroscopy. J. Chromatogr. Sci. 29, 324-330 https://doi.org/10.1093/chromsci/29.8.324
  19. Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. C., and Pariza, M. W. (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853-858 https://doi.org/10.1007/s11745-997-0109-x
  20. Pariza, M. W. (2004) Perspective on the safety and effectiveness of conjugated linoleic acid. Am. J. Clin. Nutr. 79, 1132S-1136S https://doi.org/10.1093/ajcn/79.6.1132S
  21. Sakono, M., Miyanaga, F., Kaunhara, S., Yamauchi, K., Fukuda, N., Watanabe, K., Iwata, T., and Sugano, M. (1999) Dietary conjugated linoleic acid reciprocally modifies ketogenesis and lipid secretion by the rat liver. Lipids 34, 997-1000 https://doi.org/10.1007/s11745-999-0450-0
  22. SAS Institute Inc. (1996) SAS/STAT User`s Guide: Version 6. SAS Institute, Inc., Cary, NC
  23. Sirri, F., Tallarico, N., Meluzzi, A., and Franchini, A. (2003) Fatty acid composition and productive traits of broiler fed diets containing conjugated linoleic acid. Poult. Sci. 82, 1356-1361 https://doi.org/10.1093/ps/82.8.1356
  24. Smith, S. B., Hively, T. S., Cortese, G. M., Han, J. J., Chung, K. Y., and Castenada, P. (2003) Conjugated linoleic acid depress the $\Delta^9$ desaturase index and stearyl coenzyme A desaturase activity in porcine subcutaneous adipose tissue. J. Anim. Sci. 80, 2110-2115
  25. Szymczyk, B., Pisulewski, P. M., Szczurek, W., and Hanczakowski, P. (2001) Effect of conjugated linoleic acid on growth performance, feed conversion efficiency and subsequent carcass quality in broiler chickens. Br. J. Nutr. 85, 465-473 https://doi.org/10.1079/BJN2000293
  26. Takahashi, K., Kawamata, K., Akiba, Y., Iwata, T., and Kasai, M. (2002) Influence of dietary conjugated linoleic acid isomers on early inflammatory responses in male broiler chickens. Br. Poult. Sci. 43(1), 47-53
  27. Thiel-Cooper, R. I., Parrish, F. C., Spark, J. C., Wiegand, B. R., and Muller, R. C. (2001) Conjugated linoleic acid changes swine performance and carcass composition. J. Anim. Sci. 79, 1821-1828
  28. Yuraweez, M. P., Hood, J. K., Mossoba, M. M., Roachanol, J. A., and Ku, Y. (1995) Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid. Lipids 30, 595-598 https://doi.org/10.1007/BF02536995

Cited by

  1. Comparison of Physiological Changes in Broiler Chicken Fed with Dietary Processed Sulfur vol.20, pp.2, 2013, https://doi.org/10.11002/kjfp.2013.20.2.278
  2. Effect of Conjugated Linoleic Acid Feeding on the Growth Performance and Meat Fatty Acid Profiles in Broiler: Meta-analysis vol.26, pp.7, 2013, https://doi.org/10.5713/ajas.2013.13071