한국정밀공학회지 (Journal of the Korean Society for Precision Engineering)

  • 제26권3호
  • /
  • Pages.137-143
  • /
  • 2009
  • /
  • 1225-9071(pISSN)
  • /
  • 2287-8769(eISSN)
한국정밀공학회 (Korean Society for Precision Engineering)
권호 표지

쾌속 조형 기술을 이용한 바이오리액티의 개발

Development of Bioreactor by Rapid Prototyping Technology

  • 박정훈 (충북대학교 정밀기계공학과) ;
  • 이승재 (충남대학교 BK21 메카트로닉스 사업단) ;
  • 이인환 (충북대학교 기계공학부) ;
  • 조동우 (포항공과대학교 기계공학과) ;
  • 이종원 (가톨릭의대성형외과)
  • Park, Jeong-Hun (Department of Precision Mechanical Engineering, Chungbuk National Univ.) ;
  • Lee, Seung-Jae (BK21 Mechatronics groups, Chungnam National Univ.) ;
  • Lee, In-Hwan (School of Mechanical Engineering, Chungbuk National Univ.) ;
  • Cho, Dong-Woo (Department of Mechanical Engineering, POSTECH) ;
  • Rhie, Jong-Won (Department of Plastic Surgery, College of medicine, Catholic Univ.)
  • 발행 : 2009.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

It has been reported that mechanical stimulation takes a role in improving eel/ growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate cell-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly For dynamic cell-culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell-culture using this agarose gel and developed bioreactor in 3 days.

키워드

참고문헌

  1. Mauck, R. L., Soltz, M. A., Wang, C. C., Wong, D. D., Chao, P. H., Valhmu, W. B., Hung, C. T. and Ateshian, G. A., "Functional Tissue Engineering of Articular Cartilage Through Dynamic Loading of Chondrocyte-Seeded Agarose Gels," Journal of Biochemical Engineering, Vol. 122, Issue 3, pp. 252-260, 2000 https://doi.org/10.1115/1.429656
  2. Kisiday, J. D., Jin, M., DiMicco, M. A., Kurz, B. and Grodzinsky, A. J., "Effects of dynamic compressive loading on chondrocyte biosynthesis in selfassemblingpe ptide scaffolds," Journal of Biomechanics, Vol. 37, Issue 5, pp. 594 -604, 2004
  3. Meyer, U., Buchter, A., Nazer, N. and Wiesmann, H. P., "Design and performance of a bioreactor system for mechanically promoted three-dimensional tissue engineering," British Journal of Oral and Maxillofacial Surgery, Vol. 44, No. 2, pp. 134-140, 2006 https://doi.org/10.1016/j.bjoms.2005.05.001
  4. Nugent-Derfus, G. E., Takara, T., O'Neill, J. K., Cahill, S. B., Gortz, S., Pong, T., Inoue, H., Aneloski, N. M., Wang, W. W., Vega, K. I., Klein, T. J., Hsieh-Bonassera, N. D., Bae, W. C., Burke, J. D., Bugbee, W. D. and Sah, R. L., "Continuous passive motion appilied to whole joints stimulates chondrocyte biosynthesis of PRG41," OsteoArthritis and Cartilage, Vol. 15, Issue 5, pp. 566-574, 2007 https://doi.org/10.1016/j.joca.2006.10.015
  5. Lee, S. J. and Cho, D. W., "Solid Freeform Fabrication Technique in Tissue Engineering," Journal of the Korean Society for Precision Engineering, Vol. 23, No. 12, pp. 7-15, 2006
  6. Khang, G., Kim, M. S., Min, B. H., Lee, I., Rhee, J. M. and Lee, H. B., "Scaffold for Tissue Engineering," Tissue Engineering and Regenerative Medicine, Vol. 3, No. 4, pp. 376-395, 2006
  7. Knight, M. M., Ghori, S. A., Lee, D. A. and Bader, D. L., "Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression," Medical Engineering & Physics, Vol. 20, No. 9, pp. 684-688, 1998 https://doi.org/10.1016/S1350-4533(98)00080-0
  8. Awad, H. A., Wickkham, M. Q., Leddy, H. A., Gimble, J. M. and Guilak, F., "Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds," Biomaterials, Vol. 25, Issue 16, pp. 3211-3222, 2004 https://doi.org/10.1016/j.biomaterials.2003.10.045