Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Simple and Sensitive Liquid Chromatography Electrospray Ionization Mass Spectrometry Method for Determination of Glycoalkaloids in Potato (Solanum tuberosum L.)

  • Kim, Jae-Kwang (National Academy of Agricultural Science, Rural Development Administration) ;
  • Bae, Shin-Cheol (National Academy of Agricultural Science, Rural Development Administration) ;
  • Baek, Hyung-Jin (National Academy of Agricultural Science, Rural Development Administration) ;
  • Seo, Hyo-Won (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ryu, Tae-Hun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jung-Bong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Won, So-Youn (National Academy of Agricultural Science, Rural Development Administration) ;
  • Sohn, Soo-In (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Dong-Hern (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Sun-Ju (National Institute of Horticultural and Herb Science, Rural Development Administration) ;
  • Cho, Myoung-Rae (National Academy of Agricultural Science, Rural Development Administration)
  • Published : 2009.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

A method was developed using enhanced liquid chromatography coupled with electrospray ionization mass spectrometry for the analysis and quantitation of 2 main potato glycoalkaloids, $\alpha$-chaconine, and $\alpha$-solanine, without any pre-concentration or derivatisation steps. Calibration curves generated by this technique exhibited a linear dynamic range from 0.025 to $50{\mu}g/mL$ and from 0.05 to $50{\mu}g/mL$ for $\alpha$-chaconine and $\alpha$-solanine, respectively. Matrix effects were evaluated by comparing calibration curves measured in matrix-matched and solvent-based systems. Ion suppression due to matrix effects was weak and extraction recoveries of 88 to 114% were obtained in different sample matrices spiked with analyte concentrations ranging from 15 to $35{\mu}g/mL$. Potatoes that had been genetically modified to tolerate glufosinate contained the same glycoalkaloid levels as their non-transgenic counterpart. We suggest complementing compositional comparison assessment strategy by validating quantitative analytical methods for the toxic glycoalkaloids in potato plants.

Keywords

References

  1. Novak WK., Haslberger AG. Substantial equivalence of antinutrients and inherent plant toxins in genetically modified novel foods. Food Chem. Toxicol. 38: 473-483 (2000) https://doi.org/10.1016/S0278-6915(00)00040-5
  2. Smith DB, Roddick JG, Jones JL. Potato glycoalkaloids: Some unanswered questions. Trends Food Sci. Tech. 7: 126-131 (1996) https://doi.org/10.1016/0924-2244(96)10013-3
  3. Hellenas K-E, Nyman A, Slanina P, Loof L, Gabrielsson J. Determination of potato glycoalkaloids and their aglycone in blood serum by high performance liquid chromatography. Application to pharmacokinetic studies in humans. J. Chromatogr. 573: 69-78 (1992) https://doi.org/10.1016/0378-4347(92)80476-7
  4. Morris SC, Lee TH. The toxicity and teratogenicity of Solanaceae glycoalkaloids, particularly those of the potato (Solanum tuberosum):A review. Food Technol. Aust. 36: 118-124 (1984)
  5. Kodamatani H, Saito K, Niina N, Yamazaki S, Tanaka Y. Simple and sensitive method for determination of glycoalkaloids in potato tubers by high performance liquid chromatography with chemiluminescence detection. J. Chromatogr. A 1100: 26-31 (2005) https://doi.org/10.1016/j.chroma.2005.09.006
  6. Brown MS, McDonald GM, Friedman M. Sampling leaves of young potato (Solanum tuberosum) plants for glycoalkaloid analysis. J. Agr. Food Chem. 47: 2331-2334 (1999) https://doi.org/10.1021/jf981124m
  7. Haddadin MSY, Humeid MA, Qaroot FA, Robinson RK. Effect of exposure to light on the solanine content of two varieties of potato (Solanum tuberosum) popular in Jordan. Food Chem. 73: 205-208 (2001) https://doi.org/10.1016/S0308-8146(00)00279-X
  8. Lawson DR, Erb WA, Miller AR. Analysis of Solanum alkaloids using internal standardization and capillary gas chromatography. J. Agr. Food Chem. 40: 2186-2191 (1992) https://doi.org/10.1021/jf00023a028
  9. Saito K, Horie M, Hoshino Y, Nose N, Nakazawa H. Highperformance liquid chromatographic determination of glycoalkaloids in potato products. J. Chromatogr. A 508: 141-147 (1990) https://doi.org/10.1016/S0021-9673(00)91247-0
  10. Simonovska B, Vovk I. High-performance thin-layer chromatographic determination of potato glycoalkaloids. J. Chromatogr. A 903: 219-225 (2000) https://doi.org/10.1016/S0021-9673(00)00900-6
  11. Kvasnicka F, Price KR, Ng K, Fenwick GR. Determination of potato glycoalkaloids using isotachophoresis and comparison with a HPLC method. J. Liq. Chromatogr. R. T. 17: 1941-1951 (1994) https://doi.org/10.1080/10826079408013470
  12. Kim MH, Lee JS, Shin WS, Durst RA. Flow-injection liposome immunoanalysis system for potato glycoalkaloid. Food Sci. Biotechnol. 12: 430-434 (2003)
  13. Korpan YI, Volotovsky VV, Martelet C, Renault NJ, Nazarenko EA, EI'skaya AV, Soldatkin AP. A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors. Bioelectrochemistry 55: 9-11 (2002) https://doi.org/10.1016/S1567-5394(01)00150-5
  14. Matsuda F, Morino K, Miyazawa H, Miyashita M, Miyagawa H. Determination of potato glycoalkaloids using high-pressure liquid chromatography-electrospray ionization/mass spectrometry. Phytochem. Analysis 15: 121-124 (2004) https://doi.org/10.1002/pca.755
  15. Zywicki B, Catchpole G, Draper J, Fiehn O. Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic fieldgrown potatoes. Anal. Biochem. 336: 178-186 (2005) https://doi.org/10.1016/j.ab.2004.10.013
  16. Kim JK, Shiraishi T, Fukusaki E, Kobayashi A. Quantitation of formate by solid-phase microextraction and gas chromatography-mass spectrometry utilizing a $[^{13}C]$formate internal standard. J. Chromatogr. A 986: 313-317 (2003) https://doi.org/10.1016/S0021-9673(02)01996-9
  17. Kim JK, Harada K, Bamba T, Fukusaki E, Kobayashi A. Stable isotope dilution-based accurate comparative quantification of nitrogen-containing metabolites in Arabidopsis thaliana T87 cells using in vivo $^{15}N$-isotope enrichment. Biosci. Biotech. Bioch. 69:1331-1340 (2005) https://doi.org/10.1271/bbb.69.1331
  18. Hernando MD, Ferrer C, Ulaszewska M, Garcia-Reyes JF, Molina-Diaz A, Fernandez-Alba AR. Application of high performance liquid chromatography-tandem mass spectrometry with a quadrupole/linear ion trap instrument for the analysis of pesticide residues in olive oil. Anal. Bioanal. Chem. 389: 1815-1831 (2007) https://doi.org/10.1007/s00216-007-1464-z
  19. Peksa A, Golubowska G, Rytel E, Lisinska G, Aniolowski K. Influence of harvest date on glycoalkaloids contents of three potato varieties. Food Chem. 78: 313-317 (2002) https://doi.org/10.1016/S0308-8146(02)00101-2
  20. Peksa A, Golubowska G, Rytel E, Lisinska G, Aniolowski K. Changes of glycoalkaloids and nitrate contents in potatoes during chip processing. Food Chem. 97: 151-156 (2006) https://doi.org/10.1016/j.foodchem.2005.03.035
  21. Tajner-Czopek A, Jarych-Szyszka M, Lisinska G. Changes In glycoalkaloids content of potatoes destined for consumption. Food Chem. 106: 706-711 (2008) https://doi.org/10.1016/j.foodchem.2007.06.034
  22. Kuiper HA, Kleter GA, Noteborn HPJM, Kok EJ. Substantial equivalence-an appropriate paradigm for the safety assessment of genetically modified foods? Toxicology 181-182: 427-431 (2002) https://doi.org/10.1016/S0300-483X(02)00488-2
  23. Frydecka-Mazurczyk A, Zgorska K. The content of glycoalkaloids in potato tubers. Ziemniak Polski. 4: 10-12 (1997)