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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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pH-Dependent surface-enhanced resonance Raman scattering of yeast iso-1-cytochrome c adsorbed on silver nanoparticle surfaces under denaturing conditions at pH < 3

  • Lee, So-Yeong (Department of Pharmacology, College of Veterinary Medicine, Seoul National University) ;
  • Joo, Sang-Woo (Department of Chemistry, Soongsil University) ;
  • Lee, Seong-Hoon (School of Chemistry and Molecular Engineering, Seoul National University) ;
  • Lim, Man-Ho (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National Unversity)
  • Published : 2009.04.30
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Abstract

We measured the pH-induced spectral changes of yeast iso-1-cytochrome c on silver nanoparticle surfaces using surface-enhanced resonance Raman scattering (SERRS) at 457.9 nm. At a pH of ~3, the Met80 ligand in yeast iso-1-cytochrome c is assumed to dissociate, leading to a marked conformational change as evidenced by the vibrational spectral shifts. The Soret band at ~410 nm in the UV-Vis spectrum shifted to ~396 nm at pH~3, indicating a transition from a low spin state to a high spin state from a weak interaction with a water molecule. Thus, SERRS spectroscopy can measure the pH-induced denaturalization of cyt c adsorbed on metal nanoparticle surfaces at a lower concentration with a better sensitivity than ordinary resonance Raman spectroscopy.

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References

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