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OsHSF7 gene in rice, Oryza sativa L., encodes a transcription factor that functions as a high temperature receptive and responsive factor

  • Liu, Jin-Ge (Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) ;
  • Qin, Qiu-lin (State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University) ;
  • Zhang, Zhen (Department of Horticulture, Nanjing Agricultural University) ;
  • Peng, Ri-He (Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) ;
  • Xiong, Ai-Sheng (Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) ;
  • Chen, Jian-Min (College of Bioscience and Biotechnology, Yangzhou University) ;
  • Yao, Quan-Hong (Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences)
  • Published : 2009.01.31
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Abstract

Three novel Class A genes that encode heat shock transcription factor (HSF) were cloned from Oryza Sativa L using a yeast hybrid method. The OsHSF7 gene was found to be rapidly expressed in high levels in response to temperature, which indicates that it may be involved in heat stress reception and response. Over-expression of OsHSF7 in transgenic Arabidopsis could not induced over the expression of most target heat stress-inducible genes of HSFs; however, the transcription of some HSF target genes was more abundant in transgenic plants following two hours of heat stress treatment. In addition, those transgenic plants also had a higher basal thermotolerance, but not acquired thermotolerance. Collectively, the results of this study indicate that OsHSF7 might play an important role in the response to high temperature. Specifically, these findings indicate that OsHSF7 may be useful in the production of transgenic monocots that can over-express protective genes such as HSPs in response to heat stress, which will enable such plants to tolerate high temperatures.

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References

  1. Morimoto, R.I. (1998) Regulation of the heat shock transcriptional response: cross talk between family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev. 12, 3788-3796 https://doi.org/10.1101/gad.12.24.3788
  2. Nover, L., Bharti, K., Doring, P., Mishra, S.K., Ganguli, A. and Scharf, K.D. (2001) Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need. Cell Stress Chaperones 6, 177-1898 https://doi.org/10.1379/1466-1268(2001)006<0177:AATHST>2.0.CO;2
  3. Baniwal, S.K., Bharti, K., Chan, K.Y., Fauth, M., Ganguli, A., Kotak, S., Mishra, S.K., Nover, L., Port, M., Scharf, K.D., Tripp, J., Zielinski, D. and von Koskull-Doring, P. (2004) Heat stress response in plants: a complex game with chaperones and more than 20 heat stress transcription factors. J. Biosci. 29, 471-487 https://doi.org/10.1007/BF02712120
  4. Yamanouchi, U., Yano, M., Lin, H., Ashikari, M. and Yamada, K. (2002) A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc. Natl. Acad. Sci. U.S.A. 9, 7530 -7535
  5. Schoffl, F., Prandl, R. and Reindl, A. (1998) Regulation of the heat-shock response. Plant Physiol. 117, 1135-1141 https://doi.org/10.1104/pp.117.4.1135
  6. Bharti, K., Schmidt, E., Lyck, R., Bublak, D. and Scharf, K.D. (2000) Isolation and characterization of HsfA3, a new heat stress transcription factor of Lycopersicon peruvianum. Plant J. 22, 355-365 https://doi.org/10.1046/j.1365-313x.2000.00746.x
  7. Doring, P., Treuter, E., Kistner, C., Lyck, R., Chen, A. and Nover, L. (2000) The role of AHA motifs in the activator function of tomato heat stress transcription factors HsfA1 andHsfA2. Plant Cell 12, 265-278 https://doi.org/10.1105/tpc.12.2.265
  8. Mishra, S.K., Tripp, J., Winkelhaus, S., Tschiersch, B., Theres, K., Nover, L. and Scharf, K.D. (2002) In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Genes Dev. 16, 1555-1567 https://doi.org/10.1101/gad.228802
  9. Lohmann, C., Eggers-Schumacher, G., Wunderlich, M. and Schoffl, F. (2004) Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis. Mol. Genet. Genomics 271, 11-21 https://doi.org/10.1007/s00438-003-0954-8
  10. Busch, W., Wunderlich, M. and Schoffl, F, (2005) Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana. Plant J. 41, 1-14 https://doi.org/10.1111/j.1365-313X.2004.02272.x
  11. Li, C., Chen, Q., Gao, X., Qi, B., Chen, N., Xu, S., Chen, J. and Wang, X. (2005) AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis. Sci. China C Life Sci. 48, 540-550 https://doi.org/10.1360/062005-119
  12. Nishizawa, A., Yabuta, Y., Yoshida, E., Maruta, T., Yoshimura, K. and Shigeoka, S. (2006) Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress. Plant J. 48, 535-547 https://doi.org/10.1111/j.1365-313X.2006.02889.x
  13. Charng, Y.Y., Liu, H.C., Liu, N.Y., Chi, W.T., Wang, C.N., Chang, S.H. and Wang, T.T. (2007) A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol. 143, 251-262 https://doi.org/10.1104/pp.106.091322
  14. Panikulangara, R.J., Eggers-Schumacher, G., Wunderlich, M., Stransky, H. and Schoffl, F. (2004) Galactinol synthase1: a novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis. Plant Physiol. 136, 3148-3158 https://doi.org/10.1104/pp.104.042606
  15. Storozhenko, S., Pauw, P.D., Montagu, M.V., Inze, D. and Kushnir, S. (1998) The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter. Plant Physiol. 118, 1005-1014 https://doi.org/10.1104/pp.118.3.1005
  16. Bharti, K., von Koskull-Doring, P., Bharti, S., Kumar, P., Tintschl-Korbitzer, A., Treuter, E. and Nover, L. (2004) Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with HAC1/CBP. Plant Cell 16, 1521-1535 https://doi.org/10.1105/tpc.019927
  17. Miller, G. and Mittler, R. (2006) Could heat shock transcription factors function as hydrogen peroxide sensors in plants. Ann. Bot. 98, 279-288 https://doi.org/10.1093/aob/mcl107
  18. Hong, S.W., Lee, U. and Vierling, E. (2003) Arabidopsis hot mutants de-fine multiple functions required for acclimation to high temperatures. Plant Physiol. 132, 757-767 https://doi.org/10.1104/pp.102.017145
  19. Zhang, X., Henriques, R. and Lin, S.S. (2006) Agrobacteriummediated transformation of Arabidopsis thaliana using the floral dip method. Nat. Protoc. 1, 641-646 https://doi.org/10.1038/nprot.2006.97
  20. Zhu, B., Xiong, A.S., Peng, R.H., Xu, J., Zhou, J., Xu, J.T., Jin, X.F., Zhang, Y., Hou, X.L. and Yao, Q.H. (2008) Heat stress protection in Aspen sp1 transgenic Arabidopsis thaliana. BMB Rep. 41, 382-387 https://doi.org/10.5483/BMBRep.2008.41.5.382

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