Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Phenanthrene-induced Oxidative DNA Damage of Lymphocytes and the Suppression by Ginseng Extract

페난트렌에 의한 임파구 DNA의 산화적 손상과 인삼추출물에 의한 억제

  • Yoo, Ah-Reum (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Lee, Mi-Young (Department of Medical Biotechnology, Soonchunhyang University)
  • 유아름 (순천향대학교 의료생명공학과) ;
  • 이미영 (순천향대학교 의료생명공학과)
  • Published : 2009.12.31
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Abstract

Phenanthrene ($C_{14}H_{10}$) is a polycyclic aromatic hydrocarbon with three aromatic rings, and it can be produced by incomplete combustion of fossil fuels. Comet assay was used to examine the oxidative DNA damage of lymphocytes by phenanthrene and to measure the suppressive effects of ginseng extract on the DNA damage in this investigation. The in vitro oxidative DNA damage by phenanthrene increased in a dose-dependent manner in the lymphocyte. However, the DNA damage was significantly inhibited by ascorbate. Moreover, pretreatment, cotreatment and posttreatment with ginseng extract enhanced lymphocyte resistance to the phenanthrene-induced DNA damage. Phenanthrene enhanced the generation of intracellular reactive oxygen species, and the elevated reactive oxygen species level was reduced by treatment with ginseng extract.

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References

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