Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Benzo[a]pyrene-exposed Marine Medaka

  • Woo, Seon-Ock (South Sea Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Won, Hyo-Kyoung (South Sea Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Jeon, Hye-Young (South Sea Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Kim, Bo-Ra (South Sea Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Park, Hong-Seog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yum, Seung-Shic (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
  • Published : 2009.12.31
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Abstract

Differential gene expression profiling was performed in the hepatic tissue of marine medaka fish (Oryzias javanicus) after exposure to benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), by heterologous hybridization using a medaka cDNA microarray. Thirty-eight differentially expressed candidate genes, of which 23 were induced and 15 repressed (P<0.01), were identified and found to be associated with cell cycle, development, endocrine/reproduction, immune, metabolism, nucleic acid/protein binding, signal transduction, or non-categorized. The presumptive physiological changes induced by BaP exposure were identified after considering the biological function of each gene candidate. The results obtained in this study will allow future studies to assess the molecular mechanisms of BaP toxicity and the development of a systems biology approach to the stress biology of organic chemicals.

Keywords

References

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