Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Studies on Protective Effect of Bupleurum falcatum Extract (SHI-1909) against Experimental Inflammtory Bowel Disease Model

시호추출물 (SHI-1909)의 염증성 대장염 실험동물 모델에 대한 치료효과

  • Cho, Seong-Wan (Department of Pharmaceutical Engineering, Konyang University) ;
  • Kim, Young-Kwon (Department of Biomedical Laboratory Science, Konyang University)
  • Published : 2009.03.31
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Abstract

The Efficacy of SHI-1909 was investigated in comparision with predinisolone in acetic acid and Picrylsulfonic acid solution (TNBS)-induced rat inflammatory bowel disease (IBD) for 5 days. 7% Acetic acid and 5% TNBS solution were administered with polyethylene (P.E) tube inserted to rats intracolon, which causing colitis to the rats. The acetic acid and TNBS control group (the saline treated colitic rat) exhibited ulceration and inflammation of the distal colon with formation of granuloma and pathologic connections. We checked the inflammatory parameters like rat's weight, food intake quantity change during administration. After 5 days, we sacrificed the rats and checked the colon's length, ulcer and pathologic condition. Oral treatment with SHI-1909 resulted in significant recovery of macroscopic parameters like weight and diet intake change. Especially, SHI-1909 had a more potent effect than prednisolone on macroscopic colonic damage score. We can suggest that SHI-1909 could be a promising drug in the treatment of IBD.

SHI-1909를 초산과 TNBS에 의해 rat에 유발된 염증성 대장염 모델에서 5일 동안 경구 투여 하여 대조약인 프레드니솔론과 그 치료 효능을 비교 조사하였다. 7% 초산과 5% TNBS 용액을 polyethylene 튜브로 rat의 항문에 점적하여 염증성 대장염을 유발하였으며 점적 후 초산과 TNBS 대조군은 말단의 대장부위에서 궤양과 염증 증상 같은 병적인 소견을 보여 염증성 대장염이 잘 유발되었음을 확인할 수 있었다. 약물의 투약기간 중 염증 치유 변수인 실험동물의 중량과 식이 섭취량 변화를 관찰하였으며 실험이 종료된 후에는 실험동물을 희생시킨 후 대장의 길이와 궤양, 병적인 소견을 조사하였다. SHI-1909의 투여는 중량변화와 식이 섭취량 등에서 대조약과 필적할 만한 결과를 나타내었으며 특히 대장의 손상 정도 평가에서 대조약물인 프레드니솔론보다도 더 우수한 효과를 나타내었다. 이러한 결과로부터 SHI-1909는 IBD의 치료에 가능성이 있는 치료 약물이 될 수 있을 것으로 사료된다.

Keywords

References

  1. J. Andrews et al., "Inflammatory bowel disease-its history, current status and outlook", Am. J. Med. 100:656-659, 1996.
  2. C. O. Elson et al., "The basis of current and future therapy for inflammatory bowel disease", Med. J. Aus. 160:219-225, 1994.
  3. H. D. Yuan et al., "The effects of Curcumin on the Dextran Sulfate Sodium-Induced Colitis in the Rat", Cancer prevention research 11:137-143, 2006.
  4. S. A, Weitzman et al., "Inflammation and cancer, role of phagocyte-generated oxidants in carcinogenesis", Blood 76:655-663, 1990.
  5. 안중환, 황련해독탕이 DSS로 유발된 흰쥐의 궤양성 대장염에 미치는 영향. 동국대학교 석사학위논문, 2005.
  6. 최규완, 한국인의 염증성 장질환, 대한내과학과 추계학술대회지, 1996.
  7. C. O. Elson et al., "Experimental models of inflammatory bowel disease", Gastroenterology 109:1344-1347, 1995. https://doi.org/10.1016/0016-5085(95)90599-5
  8. G. P. Morris et al., "Hapten-induced model of chronic inflammation and ulceration in the rat colon", Gastroenterology 96:795-803, 1989. https://doi.org/10.1016/S0016-5085(89)80079-4
  9. M. W. Son et al., "Effect of DA-6034, a New Flavonoid Derivative, on TNBS-Induced Colitis in the Rat", Yak hak Hoeji Vol. 42:205-213, 1998.
  10. P. Sharon et al., "Metabolism of arachidonic acid in acetic acid colitis in rats. Similarity to human inflammatory bowel disease", Gastroenterology 88:55-63, 1985. https://doi.org/10.1016/S0016-5085(85)80132-3
  11. B. O. Ahn et al., "Beneficial Effect of DA-9601, an Extract of Artemisiae Herba, on Animals Models of Inflammatory Bowel Disease", The Journal of Applied Pharmacology 5:165-173, 1997.
  12. Y. S. Kim, et al., "Effect of DA-6034, a Derivative of Flavonoid, on Experimental Animal Models of Inflammatory Bowel Disease", Archives of Pharmacal Research 22: 354-360, 1999. https://doi.org/10.1007/BF02979057
  13. M. W. Son, et al., "Effect of Taurine on TNBS-induced Inflammatory Bowel Disease in Rats", Archives of Pharmacal Research 21: 531-536, 1998. https://doi.org/10.1007/BF02975370
  14. J. L. Wallace et al., "Release of platelet-activating factor (P AF) and accelerated healing induced by a PAF antagonist in animal model of chronic colitis", Can. J. Physiol. Pharmacol. 66:422-425, 1988. https://doi.org/10.1139/y88-071
  15. R. B. Sartor et al., "Gramulomatous enterocolitis induced in rats by purified bacterial cell wall fragments", Gastroenterology 89:587-595, 1985. https://doi.org/10.1016/0016-5085(85)90455-X
  16. 최윤경, "Inhibitory Action of Sinapinic Acid on Trinitrobenzen Sulphonic Acid(TNBS)-induced Colitis", 중앙대 석사학위논문, 2006.
  17. J. Vilaseca et al., "Dietary fish oil reduces progression of chronic inflammatory lesions in a rat model granulomatous colitis", Gut 31: 539-544, 1990. https://doi.org/10.1136/gut.31.5.539
  18. 손홍문, "The Study on the Effects of Gliotoxin on TNBS-induced Colitis", 원광대학교 박사학위논문, 2003.
  19. J. M. Oh, et al., "Protects against TNBS Induced Colitis Through HO-1 Induction", The Korean J. Anat. 37:459-466, 2004.
  20. Y. Q. Ou, Recent advances in the pathogenesis of inflammatory bowel disease and its therapy, Chinese J. Digest. 23:453-454, 2003.

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