Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant and Neuronal Cell Protective Effects of Methanol Extract from Schizandra chinensis using an in vitro System

In vitro system에서 오미자 메탄올 추출물의 항산화 및 신경세포 보호효과

  • Kim, Ji-Hye (Division of Agriculture and Life Sciences and Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Chang-Ho (Division of Agriculture and Life Sciences and Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Gwi-Nam (Division of Agriculture and Life Sciences and Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kwak, Ji-Hyun (Division of Agriculture and Life Sciences and Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Agriculture and Life Sciences and Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho-Jin (Division of Agriculture and Life Sciences and Institute of Agriculture and Life Science, Gyeongsang National University)
  • 김지혜 (경상대학교 대학원 응용생명과학부 및 농업생명과학연구원) ;
  • 정창호 (경상대학교 대학원 응용생명과학부 및 농업생명과학연구원) ;
  • 최귀남 (경상대학교 대학원 응용생명과학부 및 농업생명과학연구원) ;
  • 곽지현 (경상대학교 대학원 응용생명과학부 및 농업생명과학연구원) ;
  • 최성길 (경상대학교 대학원 응용생명과학부 및 농업생명과학연구원) ;
  • 허호진 (경상대학교 대학원 응용생명과학부 및 농업생명과학연구원)
  • Published : 2009.12.31
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Abstract

In this study, the antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis were evaluated. The proximate composition and total phenolics content of the extract were as follows: 64.88% nitrogen free extract, 10.56% crude fiber, 10.22% moisture, 8.33% crude protein, 5.05% ash, 0.96% crude fat, and 83.04 mg/g of total phenolics. In assays the methanol extract of Schizandra chinensis presented ferric reducing/antioxidant power (FRAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity in a dose-dependent manner. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), the methanol extract showed protective effect against $H_2O_2$-induced neurotoxicity, and lactate dehydrogenase (LDH) release into medium was also inhibited by various concentrations of extracts (68-80%). Cell viability after treatment of the methanol extract was higher than that shown for vitamin C ($100\;{\mu}M$) using a neutral red uptake (NRU) assay. Therefore, these data suggest that the methanol extract of Schizandra chinensis may be useful for neurodegenerative diseases including Alzheimer's disease.

오미자 메탄올 추출물의 항산화 특성 및 $H_2O_2$로 유도된 신경세포 독성에 대한 보호효과를 조사하였다. ABTS radical 소거 및 FRAP방법을 이용하여 오미자 메탄올 추출물의 항산화 활성을 측정한 결과 추출물의 농도가 증가함에 따라 항산화 활성이 증가하는 농도의존적인 경향을 보였다. $H_2O_2$로 유도된 PC12 신경세포에 대한 보호효과를 측정한 결과 모든 시료에서 72-99% 정도의 신경세포 보호효과를 보였고, LDH release assay 결과 0.5 mg/mL 농도에서 47% 정도의 LDH 방출량 저해효과를 나타냈으며, neutral red uptake assay 결과 모든 농도에서 vitmain C에 비해 높은 생존율을 보였다. 본 연구 결과를 종합해 볼 때, 오미자 메탄올 추출물의 항산화력과 산화적 스트레스로부터 신경세포의 뛰어난 보호효과는 알츠하이머성 신경질환의 예방 및 치료제로서의 활용 가능성이 높다고 판단된다.

Keywords

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