Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Adipocyte-Related Genes and Transcription Factors were affected by Ganoderma lucidum during 3T3-L1 Differentiation

지방세포 분화중인 3T3-L1 세포에서 영지추출물에 의한 지방관련 유전자와 전사인자의 발현 조절

  • Lee, Chae-Woo (Dept. of Acupuncture & Moxibustion College of Oriental Medicine, Dong-Eui University) ;
  • Yoon, Hyun-Min (Dept. of Acupuncture & Moxibustion College of Oriental Medicine, Dong-Eui University) ;
  • Kang, Kyung-Hwa (Department of Oriental Physiology, College of Oriental Medicine, Dongeui University)
  • 이채우 (동의대학교 한의과대학 침구학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구학교실) ;
  • 강경화 (동의대학교 한의과대학 생리학교실)
  • Published : 2009.06.30
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Abstract

In this study, the effects of Ganoderma lucidum (GL) on fat metabolism were performed in 3T3-L1 adipocytes. The effects of GL on 3T3-L1 preadipocytes differentiation were also examined. Our results showed that GL decreased the TG content by ORO staining. To elucidate the mechanism of the effects of GL on lowering TG content in 3T3-L1 adipocytes, we examined whether GL modulate the expressions of transcription factors and adipokines related to control of energy expenditure process because adipokines regulate adipocyte mass and increased expenditure may consume much TG in adipocytes. As a result, the expression of C/$EBP{\beta}$, C/$EBP{\delta}$, C/$EBP{\alpha}$, and $PPAR{\gamma}$, genes, which induce the adipose differentiation and adipose-specific FAS, aP2, and adipsin genes, which compose fat formation were decreased. In addition, GL increased the expression of leptin, UCP2, adiponectin in 3T3-L1 adipocytes, resulting in energy homeostasis. In conclusion, GL could regulate transcript factor related to induction of adipose differentiation and control TG content by up-regulation of adipokines related to fat burn.

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