Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Efficacy of Genetic Adjuvant (Plasmid-Expressed Chicken Interleukin-6) and Chemical Adjuvant (Levamisole) on the Protective Immunity of Genetic Vaccine against Infectious Bursal Disease Virus

닭의 전염성 F낭병 바이러스 유전자백신에 의한 방어 면역에 Genetic Adjuvant (Chicken Interleukin-6)와 Chemical Adjuvant (Levamisole)의 효과

  • Park, Jeong-Ho (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Sung, Haan-Woo (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Yoon, Byung-Il (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Pak, Son-Il (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kwon, Hyuk-Moo (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 박정호 (강원대학교 수의학부대학 및 동물의학종합연구소) ;
  • 성환우 (강원대학교 수의학부대학 및 동물의학종합연구소) ;
  • 윤병일 (강원대학교 수의학부대학 및 동물의학종합연구소) ;
  • 박선일 (강원대학교 수의학부대학 및 동물의학종합연구소) ;
  • 권혁무 (강원대학교 수의학부대학 및 동물의학종합연구소)
  • Received : 2009.05.08
  • Accepted : 2009.06.04
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Infectious bursal disease (IBD) caused by the infectious bursal disease virus (IBDV) has an important economic impact on the poultry industry worldwide. This study examined the adjuvant effects of a plasmid encoding chicken interleukin-6 (pcDNA-ChIL-6) and levamisole (LMS) on in ovo prime-boost vaccination using a genetic vaccine (pcDNA-VP243) to prime in chicken followed by a killed-vaccine boost. A pcDNA-VP243 was injected into the amniotic sac alone or in combination with a pcDNA-ChIL-6 or LMS at embryonation day 18, followed by an intramuscular injection of killed IBD vaccine at 1 week of age. The chicken were orally challenged with very virulent IBDV (vvIBDV) strain at 3 weeks of age and observed for 10 days. No mortality was observed in the groups that received the pcDNA-VP243 alone and pcDNA-VP243 plus pcDNA-ChIL-6 or LMS compared to 100% mortality in unvaccinated challenge control group. However, as determined by bursal damage (the presence of IBDV RNA, B/B ratio, and lesion score), a pcDNA-VP243 alone group was superior to pcDNA-VP243 plus pcDNA-ChIL-6 or LMS groups in the protection against post-challenge. These findings suggest that in ovo priming with genetic vaccine and boosting with killed vaccine is an effective strategy for protecting chicken against vvIBDV and the addition of pcDNA-ChIL-6 or LMS did not enhance protective immunity.

닭의 전염성 F낭병 바이러스(IBDV)가 원인 바이러스인 전염성 F낭병은 전 세계 양계산업에 경제적으로 피해가 큰 중요한 질병이다. 이 연구의 목적은 닭에서 IBDV에 대한 방어면역을 유도하기 위한 in ovo 초회항원자극(priming)과 불활화백신에 의한 보강접종 방법에 항원보강제(adjuvant)로 chicken interleukin 6 (pcDNA-ChIL-6;plasmid encoding chicken interleukin-6)와 levamisole (LMS)의 효과를 조사하는 것이다. IBDV의 VP2, VP, VP3 protein을 암호화하는 유전자백신인 plasmid DNA vaccine (pcDNA-VP243) 단독 또는 pcDNA-ChIL-6 또는 LMS와 함께 18일령 부화란의 양막낭(amniotic sac)에 접종하고 부화한 1주령의 병아리에 불활화 IBD 백신을 근육 접종한 다음 3주령에 고병원성 IBDV인 SH/92 주로 공격 접종하고 10일 동안 관찰하였다. 백신하지 않은 공격접종 대조군이 100%의 폐사율을 보인 반면 pcDNA-VP243 단독 접종군과 pcDNA-VP243에 pcDNA-ChIL-6 또는 LMS를 첨가한 실험군은 모두 100%의 생존율을 나타내었다. 그러나 공격접종 후 F낭의 손상을 평가하기 위한 IBDV RNA의 검출, B/B ratio와 F낭의 병변지수(lesion score) 등을 분석한 결과 pcDNA-VP243에 pcDNA-ChIL-6 또는 LMS를 첨가한 실험군은 pcDNA-VP243 단독 접종군보다 향상된 방어효과를 나타내지 않았다. 이 실험결과는 유전자백신에 의한 in ovo 초회항원자극-불활화백신에 의한 보강접종법이 고병원성 IBDV로부터 닭을 보호하기 위한 효과적인 방법이었으나 pcDNA-ChIL-6 또는 LMS의 첨가로 인한 방어효과의 향상은 나타나지 않았다.

Keywords

References

  1. Asif, M., J.W. Lowenthal, M.E. Ford, K.A. Schat, W.G. Kimpton, and A.G. Bean. 2007. Interleukin-6 expression after infectious bronchitis virus infection in chickens. Viral Immunol. 20, 479-486 https://doi.org/10.1089/vim.2006.0109
  2. Bozi, F., G. Lackovi, A. Kovsca-Janjatovi, O. Smolec, and I. Valpoti. 2006. Levamisole synergizes experimental F4ac+ Escherichia coli oral vaccine in stimulating ileal Peyer's patch T cells in weaned pigs. J. Vet. Pharmacol. Ther. 29, 199-204 https://doi.org/10.1111/j.1365-2885.2006.00731.x
  3. Chang, H.C., T.L. Lin, and C.C. Wu. 2001. DNA-mediated vaccination against infectious bursal disease in chickens. Vaccine 20, 328-335 https://doi.org/10.1016/S0264-410X(01)00376-0
  4. Chettle, N., J.C. Stuart, and P.J. Wyeth. 1989. Outbreak of virulent infectious bursal disease in East Anglia. Vet. Rec. 125, 271-272 https://doi.org/10.1136/vr.125.10.271
  5. Cho, S.H., P.C. Loewen, and R.R. Marquardt. 2004. A plasmid DNA encoding chicken interleukin-6 and Escherichia coli K88 fimbrial protein FaeG stimulates the production of anti-K88 fimbrial antibodies in chickens. Poult. Sci. 83, 1973-1978 https://doi.org/10.1093/ps/83.12.1973
  6. Fodor, I., E. Horvath, N. Fodor, E. Nagy, A. Rencendorsh, V.N. Vakharia, and S.K. Dube. 1999. Induction of protective immunity in chickens immunised with plasmid and encoding infectious bursal disease virus antigens. Acta. Vet. Hung. 47, 481-492 https://doi.org/10.1556/AVet.47.1999.4.8
  7. Foon, K.A., J. Yannelli, and M. Bhattacharya-Chatterjee. 1999. Colorectal cancer as a model for immunotherapy. Clin. Cancer Res. 5, 225-236
  8. Francois, A., C. Chevalier, B. Delmas, N. Eterradossi, D. Toquin, G. Rivallan, and P. Langlois. 2004. Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens. Vaccine 22, 2351-2360 https://doi.org/10.1016/j.vaccine.2003.10.039
  9. Haygreen, E.A., P. Kaiser, S.C. Burgess, and T.F. Davison. 2006. In ovo DNA immunisation followed by a recombinant fowlpox boost is fully protective to challenge with virulent IBDV. Vaccine 24, 4951-4961 https://doi.org/10.1016/j.vaccine.2006.03.060
  10. Hicks, C.R. and K.V. Turner. 1996. Fundamental concept in the design of experiments. Oxford University Press, New York, N.Y., USA.
  11. Hsieh, M.K., C.C. Wu, and T.L. Lin. 2007. Priming with DNA vaccine and boosting with killed vaccine conferring protection of chickens against infectious bursal disease. Vaccine 25, 3417-3427 https://doi.org/10.1016/j.vaccine.2007.04.087
  12. Hulse, D.J. and C.H. Romero. 2004. Partial protection against infectious bursal disease virus through DNA-mediated vaccination with the VP2 capsid protein and chicken IL-2 genes. Vaccine 22, 1249-1259 https://doi.org/10.1016/j.vaccine.2003.09.021
  13. Jin, H., Y. Li, Z. Ma, F. Zhang, Q. Xie, D. Gu, and B. Wang. 2004. Effect of chemical adjuvants on DNA vaccination. Vaccine 22, 2925-2935 https://doi.org/10.1016/j.vaccine.2003.12.026
  14. Kang, Y., H. Jin, G. Zheng, Q. Xie, J. Yin, Y. Yu, C. Xiao, X. Zhang, A. Chen, and B. Wang. 2005. The adjuvant effect of levamisole on killed viral vaccines. Vaccine 23, 5543-5550 https://doi.org/10.1016/j.vaccine.2005.07.017
  15. Kibenge, F.S.B., A.S. Dhillon, and R.G. Russell. 1988. Biochemistry and immunogenicity of infectious bursal disease virus. J. Gen. Virol. 69, 1757-1775 https://doi.org/10.1099/0022-1317-69-8-1757
  16. Kim, S.J., H.W. Sung, J.H. Han, D. Jackwood, and H.M. Kwon. 2004. Protection against very virulent infectious bursal disease virus in chickens immunized with DNA vaccines. Vet. Microbiol. 101, 39-51 https://doi.org/10.1016/j.vetmic.2004.03.009
  17. Kimball, E.S. and M.C. Fisher. 1996. Levamisole effects on major histocompatibility complex and adhesion molecule expression and on myeloid cell adhesion to human colon tumor cell lines. J. Natl. Cancer Inst. 88, 109-116 https://doi.org/10.1093/jnci/88.2.109
  18. Kwon, Y.K., I.P. Mo, H.W. Seong, M.I. Kang, H.B. Koh, J.G. Lee, and C.K. Yang. 1995. Studies on the pathogenicity of infectious bursal disease virus (SH/92) isolated in Korea. RDA. J. Agric. Sci. 37, 637-647
  19. Larsen, D.L., N. Dybdahl-Sissoko, M.W. McGregor, R. Drape, V. Neumann, W.F. Swain, D.P. Lunn, and C.W. Olsen. 1998. Coadministration of DNA encoding interleukin-6 and hemagglutinin confers protection from influenza virus challenge in mice. J. Virol. 72, 1704-1708
  20. Lewis, P.J. and L.A. Babiuk. 1999. DNA vaccines: a review. Adv. Virus Res. 54, 129-188 https://doi.org/10.1016/S0065-3527(08)60367-X
  21. Lin, A., A. Kato, Y. Otaki, T. Nakamura, E. Sasmaz, and S. Ueda. 1993. Sequence comparisons of a highly virulent infectious bursal disease virus prevalent in Japan. Avian. Dis. 37, 315-323 https://doi.org/10.2307/1591655
  22. Lukert, P.D. and Y.M. Saif. 2003. Infectious bursal disease, pp. 161-179 In Y.M. Saif, H.J. Barnes, A.M. Fadly, J.R. Glisson, L.R. McDougald, and D.E. Swayne (eds.), Disease of poultry. Iowa State University Press, Ames, IA, USA.
  23. Lynagh, G.R., M. Bailey, and P. Kaiser. 2000. Interleukin-6 is produced during both murine and avian Eimeria infections. Vet. Immunol. Immunopathol. 76, 89-102 https://doi.org/10.1016/S0165-2427(00)00203-8
  24. Min, W., H.S. Lillehoj, J. Burnside, K.C. Weining, P. Staeheli, and J.J. Zhu. 2001. Adjuvant effects of IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta4 and lymphotactin on DNA vaccination against Eimeria acervulina. Vaccine 20, 267-274 https://doi.org/10.1016/S0264-410X(01)00270-5
  25. Mller, H., M.R. Islam, and R. Raue. 2003. Research on infectious bursal disease--the past, the present and the future. Vet. Microbiol. 97, 153-165 https://doi.org/10.1016/j.vetmic.2003.08.005
  26. Mundt, E., J. Beyer, and H. Muller. 1995. Identification of a novel viral protein in infectious disease virus-infected cells. J. Gen. Virol. 76, 437-443 https://doi.org/10.1099/0022-1317-76-2-437
  27. Nishimichi, N., M. Aosasa, T. Kawashima, H. Horiuchi, S. Furusawa, and H. Matsuda. 2005. Biological activity of recombinant chicken interleukin-6 in chicken hybridoma cells. Vet. Immunol. Immunopathol. 106, 97-105 https://doi.org/10.1016/j.vetimm.2005.01.006
  28. Park, J.H., H.W. Sung, and H.M. Kwon. 2008. Evaluation of priming with inovo DNA vaccine and boosting with killed vaccine strategies for protective immunity against IBDV and effects of plasmid-encoded chicken interleukin-2 and chicken interferon-$\gamma$. In Proceedings of AVMA/AAAP 77, New Orleans, LA, USA.
  29. Renoux, G. 1980. The general immunopharmacology of levamisole. Drugs 20, 89-99 https://doi.org/10.2165/00003495-198020020-00001
  30. Roh, H.J., H.W. Sung, and H.M. Kwon. 2006. Effects of DDA, CpG-ODN, and plasmid-encoded chicken INF-$\gamma$ on protective immunity by a DNA vaccine against IBDV in chickens. J. Vet. Sci. 7, 361-368 https://doi.org/10.4142/jvs.2006.7.4.361
  31. Schneider, K., R. Klaas, B. Kaspers, and P. Staeheli. 2001. Chicken interleukin-6. cDNA structure and biological properties. Eur. J. Biochem. 268, 4200-4206 https://doi.org/10.1046/j.1432-1327.2001.02334.x
  32. Snyder, D.B., V.N. Vakharia, and P.K. Savage. 1992. Naturally occurring-neutralizing monoclonal antibody escape variants define the epidemiology of infectious bursal disease viruses in the United States. Arch. Virol. 127, 89-101 https://doi.org/10.1007/BF01309577
  33. Sun, J.H., Y.X. Yan, J. Jiang, and P. Lu. 2005. DNA immunization against very virulent infectious bursal disease virus with VP2-4-3 gene and chicken IL-6 gene. J. Vet. Med. B. Infect. Dis. Vet. Public Health 52, 1-7 https://doi.org/10.1111/j.1439-0450.2004.00813.x
  34. Tsukamoto, K., N. Tanimura, S. Kakita, K. Ota, M. Mase, K. Imai, and H. Hihara. 1995. Efficacy of three live vaccines against highly virulent infectious bursal disease virus in chickens with or without maternal antibodies. Avian. Dis. 39, 218-229 https://doi.org/10.2307/1591863
  35. Van den Berg, T.P., M. Gonze, and G. Meulemans. 1991. Acute infectious bursal disease in poultry: isolation and characterization of a highly virulent strain. Avian. Pathol. 20, 133-143 https://doi.org/10.1080/03079459108418748
  36. Wang, B., K.E. Ugen, V. Srikantan, M.G. Agadjanyan, K. Dang, Y. Refaeli, A.I. Sato, J. Boyer, W.V. Williams, and D.B. Weiner. 1993. Gene inoculation generates immune responses against human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 90, 4156-4160 https://doi.org/10.1073/pnas.90.9.4156
  37. Yin, J., H. Jin, F. Yang, Z. Ding, C. Huang, Q. Zhu, and B. Wang. 2007. Synergistic effects of adjuvants interferon-gamma and levamisole on DNA vaccination against infection with Newcastle disease virus. Viral. Immunol. 20, 288-299 https://doi.org/10.1089/vim.2006.0108