Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

MANDIBULAR BONE REGENERATION USING AUTOGENOUS SKIN-DERIVED PRECURSOR CELLS WITH A MIXED DEMINERALIZED BONE AND FIBRIN GLUE SCAFFOLD IN MINIATURE PIGS

미니돼지에서 자가 피부유래 전구세포와 탈회골 및 피브린 스케폴드를 이용한 하악골 골결손부의 골재생에 대한 연구

  • Byun, June-Ho (Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Choi, Mun-Jeong (Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Choi, Young-Jin (Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Shim, Kyoung-Mok (Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Kim, Uk-Kyu (Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Jong-Ryoul (Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Park, Bong-Wook (Department Oral & Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University)
  • 변준호 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 최문정 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 최영진 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 심경목 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김종렬 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 박봉욱 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원)
  • Received : 2009.04.13
  • Accepted : 2009.05.13
  • Published : 2009.05.30
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Abstract

Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal's mandibular defects. Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4 weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. $1{\times}10^7\;cells/100\;{\mu}l$ of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold. In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4 weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the SKP-grafted group compared to the control group. Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold can serve as a useful alternative to bone grafting technique.

Keywords

References

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