Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Acrylic Acid from Acrylonitrile by Immobilization of Arthrobacter nitroguajacolicus ZJUTB06-99

  • Shen, Mei (Institute of Bioengineering, Zhejiang University of Technology) ;
  • Zheng, Yu-Guo (Institute of Bioengineering, Zhejiang University of Technology) ;
  • Liu, Zhi-Qiang (Institute of Bioengineering, Zhejiang University of Technology) ;
  • Shen, Yin-Chu (Institute of Bioengineering, Zhejiang University of Technology)
  • Published : 2009.06.30
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Abstract

Immobilized cells of Arthrohacter nitroguajacolicus ZJUTB06-99 capable of producing nitrilase were used for biotransformation of acrylonitrile to acrylic acid. Six different entrapment matrixes were chosen to search for a suitable support in terms of nitrilase activity. Ca-alginate proved to be more advantageous over other counterparts in improvement of the biocatalyst activity and bead mechanical strength. The effects of sodium alginate concentration, $CaCl_2$ concentration, bead diameter, and ratio by weight of cells to alginate, on biosynthesis of acrylic acid by immobilized cells were investigated. Maximum activity was obtained under the conditions of 1.5% sodium alginate concentration, 3.0% $CaCl_2$ concentration, and 2-mm bead size. The beads coated with 0.10% polyethylenimine (PEI) and 0.75% glutaraldehyde (GA) could tolerate more phosphate and decrease leakage amounts of cells from the gel. The beads treated with PEI/GA could be reused up to 20 batches without obvious decrease in activities, which increased about 100% compared with the untreated beads with a longevity of 11 batches.

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References

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