Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Screening and Identification of a Streptomyces platensis YK-2, a New Transglutaminase Producer

  • Yeo, Soo-Hwan (Department of Korean Food Research for Globalization, NAAS, RDA) ;
  • Yoon, Jung-Hoon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Dong-Gun (School of Life Sciences and Biotechnology, College of National Sciences, Kyungpook National University) ;
  • Kim, Hyun-Soo (Department of Microbiology, Keimyung University)
  • Published : 2009.06.30
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Abstract

A bacterial strain, YK-2, was isolated as a producer of trans glutaminase from a forest soil sample of Daegu, Korea. The isolate showed a G+C content of 72.7 mol%, contained meso-$A_2pm$ as the cell-wall amino acid, and possessed menaquinone MK-9 ($H_6$) and menaquinone MK-9 ($H_8$) at a ratio of 6:4. The chemotaxonomic analysis, as well as phylogenetic analysis based on the 16S rDNA sequence, identified the isolate as a member of Streptomyces platensis. For transglutaminase production, the optimum medium composition was determined to be 2% glucose, 1% polypeptone, 1% soy tone, and 0.1% $MnCl_2$. The transglutaminase was stable within the pH range of 5.0-9.0 and $30-45^{\circ}C$, and the optimum pH and temperature were pH 8.0 and $45^{\circ}C$, respectively, without any requirement for $Ca^{2+}$.

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References

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