동의신경정신과학회지 (Journal of Oriental Neuropsychiatry)

  • 제20권1호
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  • Pages.21-42
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  • 2009
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  • 1226-6396(pISSN)
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  • 2234-4942(eISSN)
대한한방신경정신과학회 (The Korean Society of Oriental Neuropsychiatry)
권호 표지

청혈단(淸血丹)의 6-hydroxydopamine에 의해 유발된 독성에 대한 신경세포보호효과

The Protective Effect of Chunghyul-dan(Qingxuedan) Against 6-hydroxydopamine Induced Neurotoxicity.

  • 김광호 (경희대학교 한의과대학 한방신경정신과) ;
  • 김종우 (경희대학교 한의과대학 한방신경정신과) ;
  • 정선용 (경희대학교 한의과대학 한방신경정신과) ;
  • 조성훈 (경희대학교 한의과대학 한방신경정신과) ;
  • 오명숙 (경희대학교 약학대학 한약학과) ;
  • 황의완 (경희대학교 한의과대학 한방신경정신과)
  • Kim, Gwang-Ho (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Jong-Woo (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Chung, Sun-Yong (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Cho, Sung-Hoon (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Oh, Myun-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung-Hee University) ;
  • Hwang, Wei-wan (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University)
  • 발행 : 2009.03.30
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초록

Objectives : This Study was performed to assess the antioxidant and neuroprotective effect of Chunghyul-dan(Qingxuedan) in PC12 cells and primary rat mesencephalic dopaminergic neurons. Methods : The anioxidant effect was investigated using the DPPH radical and ABTS cation scavenging assays and total polyphenol amout of Chunghyul-dan(Qingxuedan). The neuroprotective effect of Chunghyul-dan(Qingxuedan) in PC12 cells was evaluated using MTT assay. The scavenging activity of Chunghyul-dan(Qingxuedan) on ROS production induced by 6-OHDA(6-hydroxydopamine) in PC12 cells was evaluated, as well as the attenuating effect on GSH reduction. Finally, we examined the neuroprotective effect of Chunghyul-dan(Qingxuedan) against 6-0HDA-induced toxicity in the primary culture of rat mesencephalic doperminergic neurons. Results : Chunghyul-dan(Qingxuedan) showed concentration-dependent scavenging activities in DPPH radical and ABTS cation scavenging assays and it was not cytotoxic to PC12 cells. In postand co-treatment, Chunghyul-dan(Qingxuedan) protected PC12 cells from the 6-OHDA induced toxicity at 50 and 100 ${\mu}$g/mL significantly. And Chunghyu!-dan(Qingxuedan) decreased the 6-OHDA induced ROS production at a dose dependent manner, while increaing the 6-OHDA induced GSH reduction at 50 and 100 ${\mu}$g/mL significantly. Finally, Chunghyul-dan(Qingxuedan) showed signicant protection of rat mescencephalic dopaminergic neurons from 6-OHDA at 1 ${\mu}$g/mL. Conclusions : These results demonstrate that Chunghyul-dan(Qingxuedan) has the antioxidant and neuroprotective effect against 6-0HDA induced cytotoxicity through decreasing ROS production and increasing GSH reduction.

키워드

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