Molecular & Cellular Toxicology

  • 제5권2호
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  • Pages.99-107
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  • 2009
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  • 1738-642X(pISSN)
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  • 2092-8467(eISSN)
대한독성유전 단백체학회 (The Korean Society of Toxicogenomics and Toxicoproteomics)
권호 표지

Differential Gene Expression Induced by Naphthalene in Two Human Cell Line, HepG2 and HL-60

  • Kim, Youn-Jung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Song, Mee (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Song, Mi-Kyung (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Youk, Da-Young (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Choi, Han-Saem (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Sarma, Sailendra Nath (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology)
  • 발행 : 2009.06.30
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초록

Naphthalene is bicyclic aromatic compound that is widely used in various domestic and commercial applications including lavatory scent disks, soil fumigants and moth balls. Exposure to naphthalene results in the development of bronchiolar damage, cataracts and hemolytic anemia in humans and laboratory animals. However, little information is available regarding the mechanism of naphthalene toxicity. We investigated gene expression profiles and potential signature genes in human hepatocellular carcinoma HepG2 cells and human promyelocytic leukemia HL-60 cells after 3 h and 48 h incubation with the IC$_{20}$ and IC$_{50}$ of naphthalene by using 44 k agilent whole human genome oligomicroarray and operon human whole 35 k oligomicroarray, respectively. We identified 616 up-regulated genes and 2,088 down-regulated genes changed by more than 2-fold by naphthalene in HepG2 cells. And in HL-60, we identified 138 up-regulated genes and 182 down-regulated genes changed by more than 2-fold. This study identified several interesting targets and functions in relation to naphthalene-induced toxicity through a gene ontology analysis method. Apoptosis and cell cycle related genes are more commonly expressed than other functional genes in both cell lines. In summary, the use of in vitro models with global expression profiling emerges as a relevant approach toward the identification of biomarkers associated with toxicity after exposure to a variety of environmental toxicants.

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참고문헌

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