Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Medicinal Plant Water Extracts on Glucose-regulating Enzyme Activities in Goto-Kakizaki Rat Liver Cytosol

약용식물 물 추출물이 Goto-Kakizaki 흰쥐의 간 세포액에서 당대사 관련효소 활성에 미치는 영향

  • Kim, Dae-Jung (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • You, Jin-Kyoun (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Seo, Dong-Joo (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Jeong-Mi (Borinara Co., Ltd.) ;
  • Choe, Myeon (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
  • 김대중 (강원대학교 식물생명공학) ;
  • 정미자 (강원대학교 BK21사업단(뉴트라슈티컬바이오)) ;
  • 유진균 (강원대학교 식물생명공학) ;
  • 서동주 (강원대학교 식물생명공학) ;
  • 김정미 (보리나라 기업부설 연구소) ;
  • 최면 (강원대학교 식물생명공학)
  • Published : 2009.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

We have studied the anti-diabetic effects of medicinal plant water extracts on hepatic glucose-regulating enzymes such as glucokinase (GK) and acetyl-CoA carboxylase (ACC). $\alpha$-Glucosidase inhibitor is usually used to prevent and treat type II diabetes; thus, anti-$\alpha$-glucosidase activity of medicinal plant water extracts was assayed. The hepatic cytosol faction of a type II diabetic animal (Goto-Kakizaki rat) was used in GK and ACC activity assays. The medicinal plants were Lycium chinense (JGP), Discorea japonica Thunb. (SY), Pyrus pyrifolia (YSB), Cornus officinalis (SSY), Paeonia suffruticosa ANDR. (MDP), Cordyceps militaris (DCH), and Acanthopanax senticosus (GSO). JGP, SY, YSB, and SSY water extracts increased the hepatic GK activity and all medicinal plant water extracts led to an increase in hepatic ACC activity. YSB, SSY, MDP, and GSO water extracts showed significantly higher anti-$\alpha$-glucosidase activity than control samples. The highest anti-$\alpha$-glucosidase activity was observed in GSO water extract and the anti-$\alpha$-glucoside activity was higher than that of Acarbose (reference $\alpha$-glucosidase inhibitor). We suggest that JGP, SY, YSB, and SSY water extracts may exert an anti-diabetic effect by enhancing the glucose metabolism and that YSB, MDP and GSO may be used as natural $\alpha$-glucosidase inhibitors in type II diabetic conditions. Increased ACC activity by plant water extracts may provide additional anti-diabetic effect.

본 연구에서는 유전적으로 제2형 당뇨를 가진 Goto-Kakizaki 흰쥐의 간 세포액에 함유된 당 이용 대사 관련 주요 효소인 glucokinase(GK)와 acetyl CoA carboxylase (ACC)에 약용 식물 물 추출물들이 어떤 영향을 미치는지 알아보았다. $\alpha$-Glucosidase 저해제는 제2형 당뇨 치료와 예방을 위해 사용되어져 오고 있다. 따라서 약용식물 물 추출물들의 $\alpha$-glucosidase 저해 활성을 알아보았다. Goto-Kakizaki 흰쥐의 간 세포액 분획물을 GK과 ACC 활성을 알아보기 위해 사용하였다. 본 실험에 사용된 약용 식물들은 Lycium chinense(JGP), Discorea japonica Thunb.(SY), Pyrus pyrifolia(YSB), Cornus officinalis(SSY), Paeonia suffruticosa ANDR.(MDP), Cordyceps militaris(DCH) 그리고 Acanthopanax senticosus(GSO)였다. 지골피(JGP), 산약(SY), 야생배(YSB) 그리고 산수유(SSY) 물 추출물들은 GK 활성을 증가시켰고, 실험에 사용된 모든 약용식물 물추출물들은 간 ACC 활성을 증가시켰다. 야생배(YSB), 산수유(SSY), 목단피(MDP) 그리고 가시오가피(GSO) 물 추출물들은 대조군보다 현저히 높은 $\alpha$-glucosidase 활성 억제를 보여주었다. $\alpha$-Glucosidase 활성을 가장 억제시킨 것은 GSO 물 추출물이었고, 그 억제율은 $\alpha$-glucosidase 치료제인 acarbose보다 높았다. 따라서 제2형 당뇨에서 지골피 (JGP), 산약(SY), 야생배(YSB) 그리고 산수유(SSY) 물 추출물들은 글루코스 대사과정을 활성화시켜 항당뇨 효과를 나타내는 천연 소재일 것으로 생각되고 야생배(YBS), 목단피(MDP) 그리고 가시오가피(GSO)는 천연 $\alpha$-glucosidase 저해제로써 사용될 수 있을 것이다. 약용식물 물 추출물에 의한 증가된 ACC 활성은 더해진 항당뇨 효과를 나타낼 것이다.

Keywords

References

  1. Chen MS, Kao CS, Chang CJ. 1991. Prevalence and risk factors of diabetic retinopathy among non-insulin-dependent diabetic subjects. Am J Ophthalmol 114: 723-730.
  2. Korean Ministry of Health and Welfare. 2006. Report on 2005 National Health and Nutrition Survey. Ministry of Health and Welfare, Seoul.
  3. Tuomi T. 2005. Type 1 and type 2 diabetes: what do they have in common? Diabetes 54: S40-S45. https://doi.org/10.2337/diabetes.54.suppl_2.S40
  4. Georg P, Ludvik B. 2000. Lipids and diabetes. J Clin Basic Cardiol 3: 159-162.
  5. Edward SH, Raffaele N. 1998. Present knowledge in nutrition. 7th ed. ILSI Press, Washington, DC, USA. p 452-462.
  6. Hess B, Brand K. 1974. Tissue and cell fractionation. In Methods of Enzymatic Analysis. 2nd ed. Hans UB, ed. Academic Press, New York and London. p 407-409.
  7. Sharma C, Manjechwar R, Weinhouse S. 1963. Effects of diet and insulin on glucose-adenosine triphosphate phosphotransferase of rat liver. J Biol Chem 238: 3840-3845.
  8. Colowick SP, Kaplan NO. 1981. Acetyl CoA carboxylase from rat liver. In Methods in Enzymology. Lowenstein JM, ed. Academic Press, New York, USA. Vol 71, p 5-15.
  9. Jung UJ, Baek NI, Chung HG, Bang MH, Yoo JS, Jeong TS, Lee KT, Kang YJ, Lee MK, Kim HJ, Yeo JY, Choi MS. 2007. The anti-diabetic effects of ethanol extract from two variants of Artemisia princeps Pampanimi in C57BL/ KsJ-db/db mice. Food Chem Toxicol 45: 2022-2029. https://doi.org/10.1016/j.fct.2007.04.021
  10. Thampy GK, Haas MJ, Mooradian AD. 2000. Troglitazone stimulate acetyl-CoA carboxylase activity through a post-translational mechanism. Life Sci 68: 699-708. https://doi.org/10.1016/S0024-3205(00)00973-5
  11. Harwood HJ Jr, Petras SF, Shelly LD, Zaccaro LM, Perry DA, Makowski MR, Hargrove DM, Martin KA, Tracey WR, Chapman JG, Magee WP, Dalvie DK, Soliman VF, Martin WH, Mularski CJ, Eisenbeis SA. 2003. Isozymenonselective N-substituted biopiperidylcarboxamide acetyl-CoA carboxylase inhibitors reduce tissue malonyl-CoA concentrations, inhibit fatty acid synthesis, and increase fatty acid oxidation in cultured cells and in experimental animals. J Biol Chem 278: 37099-37111 https://doi.org/10.1074/jbc.M304481200
  12. Clark RF, Zhang T, Wang X, Wang R, Zhang X, Camp HS, Beutel BA, Sham HL, Gu YG. 2007. Phenoxy thiazole derivatives as potent and selective acetyl-CoA carboxylase 2 inhibitors: Modulation of isozyme selectivity by incorporation of phenyl ring substituents. Bioorg Med Chem Lett 17: 1961-1965. https://doi.org/10.1016/j.bmcl.2007.01.022
  13. Shinde P, Srivastava SK, Odedara R, Tuli D, Munshi S, Patel J, Zambad SP, Sonawane R, Gupta RC, Chauthaiwale V, Dutt C. 2009. Synthesis of spiro[chroman-2,4'-piperidin]- 4-one derivatives as acetyl-CoA carboxylase inhibitors. Bioorg Med Chem Lett 19: 949-953. https://doi.org/10.1016/j.bmcl.2008.11.099
  14. Chen J, Cheng YQ, Yamaki K, Li LT. 2007. Anti-$\alpha$-glucosidase activity of Chinese traditionally fermented soybean (douchi). Food Chem 103: 1091-1096. https://doi.org/10.1016/j.foodchem.2006.10.003
  15. Ogawa S, Fujieda S, Sakata Y, Ishizaki M, Hisamatsu S, Okazaki K, Ooki Y, Mori M, Itoh M, Korenaga T. 2004. Synthesis and glycosidase inhibitory activity of some N-substituted 5a-carba-${\beta}$-fuco- and ${\beta}$-galactopyranosylamines, and selected derivatives. Bioorg Med Chem 12: 6569-6579. https://doi.org/10.1016/j.bmc.2004.09.023
  16. Gao H, Kawabata J. 2005. $\alpha$-Glucosidase inhibition of 6-hydroxyflavones. Part 3: Synthesis and evaluation of 2,3,4-trihydroxybenzoyl-containing flavonoid analogs and 6-amino flavones as $\alpha$-glucosidase inhibitors. Bioorg Med Chem 13: 1661-1671. https://doi.org/10.1016/j.bmc.2004.12.010
  17. Kim SY, Kim HI, Kim TH, Im SS. 2000. SREBP-1c mediates the insulin dependent hepatic glucokinase expression. J Biol Chem 279: 30823-30829. https://doi.org/10.1074/jbc.M313223200
  18. Palsamy P, Subramanian S. 2009. Modulatory effects of resveratrol on attenuating the enzymes activities of carbohydrate metabolism in streptozotocin-nicotinamide-induced diabetic rats. Chem Biol Interact 179: 356-362. https://doi.org/10.1016/j.cbi.2008.11.008
  19. Kim OK. 2004. Antidiabetic effect of Glechoma hederacea Linnaeus in streptozotocin-induced diabetic rats. Kor J Pharmacogn 35: 300-308.
  20. Iynedjian PB, Gjinovei A, Renold AC. 1988. Stimulation by insulin of glucokinase gene transcription in liver of diabetic rats. J Biol Chem 263: 740-744.
  21. Joo CN, Yoon SH, Lee HS, Kim YK, Koo JH, Lee HB. 1992. Study on the hypoglycemic action of ginseng saponin on streptozotocin induced diabetic rats (Ⅱ). Kor J Ginseng Sci 16: 198-209.
  22. Joo CN, Kim SJ. 1993. Hypoglycemic action of fat soluble fraction of Panax ginseng C.A. meyer in streptozotocin induced diabetic rats. Kor J Ginseng Sci 17: 101-108.
  23. Lee HA, Sim HS, Choi KJ, Lee HB. 1998. Hypoglycemic action of red ginseng components (II): investigation of the effect of fat soluble fraction from red ginseng on enzymes related to glucose metabolism in cultured rat hepatocytes. Kor J Ginseng Sci 22: 51-59.
  24. Mertes G. 1998. Efficacy and safety of acarbose in the treatment of type 2 diabetes: data from a 2-year surveillance study. Diabetes Res Clin Pract 40: 63-70. https://doi.org/10.1016/S0168-8227(98)00045-X
  25. Stand E, Baumagarsi HJ, Fchtenbusch M, Stemplinger J. 1999. Effect of acarbose on additional insulin therapy in type 2 diabetic patients with late failure of sulphonylurea therapy. Diabetes Obes Metab 1: 215-220. https://doi.org/10.1046/j.1463-1326.1999.00021.x
  26. Hanefeld M. 1998. The role of acarbose in the treatment of non-insulin-dependent diabetes melilite. J Diabetes Complications 12: 228-237. https://doi.org/10.1016/S1056-8727(97)00123-2
  27. Kwon YI, Apostolidis E, Shetty K. 2008. In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresource Technol 99: 2981-2988. https://doi.org/10.1016/j.biortech.2007.06.035
  28. Oku T, Yamada M, Nakamura M, Sadamori N, Nakamura S. 2006. Inhibitory effects of extractive from leaves of Morusalba on human and rat small intestinal disaccharidase activity. Br J Nutr 95: 933-938. https://doi.org/10.1079/BJN20061746

Cited by

  1. Effects of Mixed Extract from Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus on Glucose-Regulating Enzymes of HepG2 in Hyperglycemic Conditions vol.39, pp.9, 2010, https://doi.org/10.3746/jkfn.2010.39.9.1257
  2. Effects of medicinal herb water extracts on expression of hepatic glucokinase, pyruvate dehydrogenase and acetyl-CoA carboxylase mRNA vol.46, pp.2, 2013, https://doi.org/10.4163/kjn.2013.46.2.119
  3. Antioxidant Activities and α-Glucosidase Inhibitory Effect of Water Extracts from Medicinal Plants vol.21, pp.3, 2013, https://doi.org/10.7783/KJMCS.2013.21.3.197