한국응용곤충학회지 (Korean journal of applied entomology)

  • 제48권3호
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  • Pages.369-375
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  • 2009
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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알기생봉(Trichogramma sp. Nabis101)의 저온 발육 휴지와 단기 저장 기술

Effect of a Low Temperature-Induced Quiescence on Short Term Storage of an Egg Parasitoid, Trichogramma sp. Nabis 101

  • 김용균 (안동대학교 생명자원과학과) ;
  • 허혜정 (안동대학교 생명자원과학과) ;
  • 김근섭 (안동대학교 생명자원과학과) ;
  • 함은혜 ((주) 나비스 중앙연구소) ;
  • 김지원 ((주) 나비스 중앙연구소) ;
  • 강성영 ((주) 나비스 중앙연구소) ;
  • 권기면 ((주) 나비스 중앙연구소)
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University) ;
  • Heo, Hye-Jung (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Geun-Seob (Department of Bioresource Sciences, Andong National University) ;
  • Hahm, Eun-Hye (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Kim, Ji-Won (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Kang, Sung-Young (Agricultural Environment Research Center, NABIS Co., Ltd.) ;
  • Kwon, Ki-Myeon (Agricultural Environment Research Center, NABIS Co., Ltd.)
  • 발행 : 2009.09.30
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초록

상용화된 Trichogramma sp. Nabis101은 알 기생봉으로 대상 기주 범위가 넓어 해충 방제용으로 농생태계에 방사되고 있다. 더욱이 최근에 미생물농약과 혼용 기술이 개발되어 이들의 적용 확대가 기대되고 있다. 그러나 아직 이 기생봉의 정확한 종 동정이 되어 있지 않다. 또한 비교적 불규칙적 수요에 대응하여 생존력과 기생 능력 차이 없이 기생봉을 저장할 수 있는 기술이 요구되고 있다. 본 연구는 이 기생봉의 internal transcribed spacer (ITS)의 염기서열을 결정하였다. 이를 토대로 이 기생봉의 종 동정을 실시한 결과 T. brasiliensis와 가장 높은 유사성을 나타냈다. 이 기생봉의 저장은 기생 초기의 기주 알을 $10^{\circ}C$에서 보관하는 기술이 결정되었다. 이 조건에서 기생봉은 5주 동안 생존력, 성비, 수명 및 기생능력의 큰 저하 없이 유지될 수 있었다.

A commercial egg parasitoid, Trichogramma sp. Nabis101, was released into agricultural cultivating areas in Korea due to its wide host spectrum against insect pests. Moreover, an application technique has been recently developed to enhance its control efficacy by mixture treatment with a microbial control agent. Despite its expansion of commercial availability, any genetic identification on this commercial strain was not determined. Also, to meet inconsistent demands from consumers, the live parasitoids need to be stored without significant loss of their survival and parasitic activity. This study determined nucleotide sequence of internal transcribed spacer (ITS) of the wasp species. The identified ITS sequences indicate that this wasp species is most similar to T brasiliensis. Optimal storage condition of this wasp required young parasitized stage at $10^{\circ}C$. Under these conditions, survival, sex ratio, longevity, and parasitic behavior were not much impaired for 5 weeks.

키워드

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피인용 문헌

  1. Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis vol.53, pp.3, 2014, https://doi.org/10.5656/KSAE.2014.06.0.012