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곤충병원성진균의 총 페놀 함량 및 DPPH 라디칼 소거능

Total Phenol Contents and DPPH Radical Scavenging Activity of Entomopathogenic Fungi

  • 이기만 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 남성희 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 송하석 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 여주홍 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이광길 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 배윤환 (대진대학교 자연과학대학 생명과학과)
  • Lee, Ki-Man (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Song, Ha-Suk (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yeo, Joo-Hong (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Bae, Yoon-Hwan (Department of Life Science, College of Natural Sciences, Daejin University)
  • 발행 : 2009.09.30

초록

곤충병원성진균 20종의 총 페놀 함량, 항산화활성 및 항균활성간의 관계를 조사하기 위해 본 실험을 수행하였다. 총 페놀 함량은 Aspergillus flavus에서 $553.0{\pm}52.15{\mu}g/g$, A. parasiticus에서 $529.9{\pm}60.10{\mu}g/g$으로 가장 높게 나타났으며, 다른 균주는 $26.6{\sim}121.9{\mu}g/g$으로 나타났다. DPPH(1, 1-diphenyl-2-picrylhydrazyl) radical 소거능은 대부분의 균주에서 나타났으며 다른 균주에 비하여 A. flavus와 A. parasiticus에서는 각각 $90.9{\pm}2.90%$$77.9{\pm}4.13%$로 높게 나타나 항산화활성과 총 페놀 함량 간 높은 연관성이 나타났다. 항균활성은 모든 식중독균에 대하여 나타났으며 Listeria monocytogenes와 Escherchia coli에 대하여 특히 강하였다.

This study was conducted to investigate the total phenol contents, antioxidative activities and antibacterial activities of twenty species of entomopathogenic fungi. The total phenol content was highest in Aspergillus flavus ($553.0{\pm}52.15{\mu}g/g$) and A. parasiticus ($529.9{\pm}60.10{\mu}g/g$). On the other hand those in other strains were within the range of $26.6{\sim}121.9{\mu}g/g$. The antioxdative activity was shown in the most of strains and the highest DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging activity was observed in A. flavus ($90.9{\pm}2.90%$) and A. parasiticus ($77.9{\pm}4.13%$). This result indicated that the antioxidative activities were very correlated with the total phenol contents. The antibacterial activitiy was found in the every tested pathogenic bacteria. Especially, the antibacterial activity was strongest against Listeria monocytogenes and Escherchia coli.

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