Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Evaluation of Efficacy of Bacteriophage CJø07 against Salmonella enteritidis Infection in the SPF Chicks

박테리오파아지 CJø07의 Salmonella enteritidis 감염에 대한 SPF 병아리에서의 효능 평가

  • Received : 2010.09.07
  • Accepted : 2010.09.20
  • Published : 2010.09.30
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Abstract

In the present study we report in vivo inhibitory potential of single strain of bacteriophage ($CJ{\phi}07$) in day-old SPF chicks experimentally infected with Salmonella enteritidis (SE). The bacteriophages prepared by feed additives and drinking water were given to chicks for 20 days starting prior 10 days before challenge with SE. Chicks were euthanized at 10 days after challenge for quantitative salmonella isolation from intestine and determination of environmental contamination level of salmonella. Bacteriophage therapy as additives in feed and drinking water resulted in significant inhibition of the SE replication in intestines of SPF chickens (P<0.05). In addition, environmental contamination by SE fecal shedding was decreased in bacteriophage-treated birds. Therefore, bacteriophage $CJ{\phi}07$ examined in this study may be a plausible alternative to antibiotics for the reduction of salmonella infection both in poultry.

본 연구에서는 SE에 특이적인 박테리오파아지($CJ{\phi}07$)의 사료 및 음수 투여가 어린 병아리의 SE 감염에 따른 장내 균증식과 체외 균배출에 미치는 영향을 평가하고자 실시하였다. 박테리오파아지를 투여한 SPF 병아리에 SE균을 공격 접종 한 뒤, 공격 접종 10일 후 채취한 맹장 분변에 존재하는 SE균 수를 측정한 결과, 박테리오파아지를 투여한 병아리의 맹장 분변 내 SE균 수는 양성 대조군에 비하여 유의성 있게 감소하였다(P<0.05). 또한, 공격 접종 10일 후 사육 시설 표면의 SE균 오염도를 측정한 결과, 박테리오파아지를 투여한 그룹의 사육 시설에서 채취한 환경 시료의 SE균 오염율(50%)이 박테리오파아지를 투여하지 않은 그룹의 SE균 환경 오염율(100%)에 비하여 감소되는 것을 통해 박테리오파아지 $CJ{\phi}07$에 의한 장내 균 증식과 체외 균 배출 억제 효능이 확인되었다. 따라서 박테리오파아지 $CJ{\phi}07$는 항생제 대체 물질로서 가금에서의 SE 감염을 효과적으로 방어할 수 있을 것으로 판단된다.

Keywords

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