Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effect of Acanthopanax extract on the DNA and erythrocyte damage induced by herbicides

제초제로 인한 DNA와 적혈구 손상에 미치는 오가피 추출물의 효과

  • Seo, Yoo-Na (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Kim, Jum-Ji (Department of Medical Biotechnology, Soonchunhyang University) ;
  • Sung, Kwang-Soo (Sushin Ogapy Co., Ltd) ;
  • Lee, Mi-Young (Department of Medical Biotechnology, Soonchunhyang University)
  • 서유나 (순천향대학교 의료생명공학과) ;
  • 김점지 (순천향대학교 의료생명공학과) ;
  • 성광수 ((주) 수신오가피) ;
  • 이미영 (순천향대학교 의료생명공학과)
  • Received : 2001.11.05
  • Accepted : 2010.12.17
  • Published : 2010.12.31
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Abstract

In order to investigate whether the ethanol extract of Acanthopanax sp. might inhibit herbicide-induced DNA damage and erythrocyte damage, the suppression of the oxidative DNA damage of lymphocyte and erythrocyte damage in the presence of the extract were evaluated by comet assay and hemolysis assay, respectively. Phenoxy herbicides, named 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) and bipyridyl herbicide paraquat induced oxidative DNA damages of lymphocytes. However, the oxidative DNA damage by 2,4-D, 2,4,5-T or paraquat was inhibited in vitro upon treating Acanthopanax extract. Moreover, the erythrocyte damage was also suppressed in vitro by Acanthopanax extract treatment.

오가피 에탄올 추출물이 제초제로 인한 DNA와 적혈구 손상을 억제할 수 있는지 알아보기 위해 코멧 어세이와 용혈반응을 사용하여 오가피 추출물 존재하에서 DNA 산화손상 억제와 적혈구 손상 억제 정도를 측정하였다. 페녹시계 제초제인 2,4-D (2,4-dichlorophenoxyacetic acid), 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) 그리고 바이피리딜계 제초제인 paraquat은 임파구 DNA에 산화적 손상을 유발하였다. 그러나 2,4-D, 2,4,5-T, 혹은 paraquat으로 인한 DNA 산화손상은 오가피 추출물 처리에 의해 시험관에서 억제되었다. 또한 적혈구 손상도 오가피 추출물 처리에 의해 시험관에서 억제되었다.

Keywords

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