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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Transduced PEP-1-AMPK inhibits the LPS-induced expression of COX-2 and iNOS in Raw264.7 cells

  • Shin, Min-Jea (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • Lee, Yeom-Pyo (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • Kim, Dae-Won (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • An, Jae-Jin (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • Jang, Sang-Ho (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • Cho, Sung-Min (Department of Neurosurgery, Hallym University Medical Center) ;
  • Sheen, Seung-Hoon (Department of Neurosurgery, Hallym University Medical Center) ;
  • Lee, Hae-Ran (Department of Pediatrics, Hallym University Medical Center) ;
  • Kweon, Hae-Yong (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural & Agicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • Eum, Won-Sik (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center) ;
  • Cho, Yong-Jun (Department of Neurosurgery, Hallym University Medical Center) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University Medical Center)
  • Published : 2010.01.31
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Abstract

AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases.

Keywords

References

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