Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antiradical Capacities of Perilla, Sesame and Sunflower Oil

  • Hong, Sun-Hee (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Kim, Mi-Jin (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Oh, Chan-Ho (Department of Food & Biotechnology, Woosuk University) ;
  • Yoon, Suk-Hoo (Korea Food Research Institute) ;
  • Song, Yeong-Ok (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University)
  • Published : 2010.03.31
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Abstract

The aim of this study is to examine the radical scavenging activity of perilla and sesame oil that Koreans traditionally consume. For DPPH radical scavenging activity, oil and its hexane/70% methanol extracts (ME) are used and for superoxide and hydroxyl radical scavenging activities, ME are used. Unrefined perilla oil, sesame oil, and refined sunflower oil are used. The yields for ME of perilla, sesame and sunflower oil are 0.57, 0.61, and 0.30%, respectively, and the amounts of phenolic compounds in ME of corresponding oil are 18.77, 88.64 and $0.05\;{\mu}g$ tannic acid/mg, respectively. $IC_{50}$ for DPPH scavenging activity of perilla, sesame and sunflower oil are 2.12, 1.91, and 3.35 mg/mL, respectively and those for ME of corresponding oils are 0.42, 0.07, and 43.11 mg/mL, respectively. In DPPH assay, the solvent used for oil sample is iso-octane and that for ME is methanol. Superoxide anion scavenging activity of ME of perilla, sesame and sunflower oil tested at 1 mg/mL concentration are 21.10, 13.25, and 3.14%, respectively. Hydroxyl radical scavenging activities of those samples tested at 1 mg/mL concentration are 86.08, 93.30, and 93.17%, respectively. In summary, the refining process seems to remove the phenolic compound during oil processing. Antiradical substances in perilla and sesame oils responsible for scavenging DPPH radicals are present in the methanol fraction, while the antiradical substances in the sunflower oil are in the lipid fraction. DPPH scavenging activity of ME of sesame oil is significantly higher than that of perilla oil (p<0.05). However, superoxide anion scavenging capacity of ME of perilla oils was found to be greater than that of both sesame and sunflower oils (p<0.05).

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References

  1. Assmann G, de Backer G, Bagnara S, Betteridge J, Crepaldi G, Fernandez-Cruz A, Godtfredsen J, Bacotot B, Paoletti R, Renaud S, Ricci G, Rocha E, Trautwein E, Urbianti G, Vaerla G, Williams C. 1997. International consensus statement on olive oil and the Mediterranean diet: implications for health in Europe. Eur J Cancer Prev 6: 418-421. https://doi.org/10.1097/00008469-199710000-00002
  2. Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, Brighenti F. 2003. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in virto assays. J Nutr 133: 2812-2819. https://doi.org/10.1093/jn/133.9.2812
  3. Owen RW, Mier W, Giacosa A, Hull WE, Spiegelhader B, Bartsch H. 2000. Phenolic compounds and squalene in olive oils; the concentration and antioxidant potential of total phenols simple phenols, secoiridoids, lignin and squalene. Food Chem Toxicol 38: 647-658. https://doi.org/10.1016/S0278-6915(00)00061-2
  4. Lee OH, Lee BY, Kim YC, Shetty K, Kim YC. 2008. Radical scavenging-linked antioxidant activity of ethanolic extracts of diverse types of extra virgin olive oils. J Food Sci 73: 519-525. https://doi.org/10.1111/j.1750-3841.2008.00865.x
  5. Ikeda A, Inui K, Fukuta Y, Sugano M. 1995. Effects of interavenous perilla oil imulsion on nutritional status, polyunsaturated fatty acid composition of tissue phospholipids and thromboxane A2 production in streptozotocin-induced diabetic rats. Nutrition 11: 450-455.
  6. Kehrer JP. 1993. Free radicals a mediators of tissue injury and disease. Crit Rev Toxicol 23: 21-48. https://doi.org/10.3109/10408449309104073
  7. Fang YZ, Yang S, Wu G. 2002. Free radicals, antioxidants, and nutrition. Nutrition 18: 872-879. https://doi.org/10.1016/S0899-9007(02)00916-4
  8. Huang D, Ou B, Priop RL. 2005. The chemistry behind antioxidant capacity assay. J Agric Food Chem 53: 1841- 1856. https://doi.org/10.1021/jf030723c
  9. Gutfinger T. 1981. Polyphenols in olive oils. JAOCS 58: 966-968. https://doi.org/10.1007/BF02659771
  10. Tarko T, Duda-Chodak A, Sroka P, Satora P, Jurasz E. 2008. Physicochemical and antioxidant properties of selected polish grape and fruit wines. Acta Sci Pol Technol Aliment 7: 35-45
  11. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T. 1989. Effects of the interaction of tannins with co-existing substances, VI. Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 37: 2016-2021. https://doi.org/10.1248/cpb.37.2016
  12. Lee JM, Chung H, Chang PS, Lee JH. 2007. Development of a method predicting the oxidative stability of edible oils using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Food Chem 103: 662-669. https://doi.org/10.1016/j.foodchem.2006.07.052
  13. Robak J, Gryglewski RJ. 1988. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 37: 837-841. https://doi.org/10.1016/0006-2952(88)90169-4
  14. Chung SK, Osawa T, Kawakish S. 1997. Hydroxyl radical- scavenging effects of apices and scavengers from brown mustar (Brassica nigra). Biosci Biotech Biochem 61: 118-123. https://doi.org/10.1271/bbb.61.118
  15. Kim EJ, Hwang SY, Son JY. 2009. Physiological activities of sesame, black sesame, perilla and olive oil extracts. J Korean Soc Food Sci Nutr 38: 280-286. https://doi.org/10.3746/jkfn.2009.38.3.280
  16. Espin J, Soler-Rivas C, Wichers H. 2000. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J Agric Food Chem 48: 648-656. https://doi.org/10.1021/jf9908188
  17. Shahidi F, Wanasundara PKJPD. 1992. Phenolic antioxidants. Crit Rev Food Sci Nutr 32: 67-103. https://doi.org/10.1080/10408399209527581
  18. Schwarz K, Bertelsen G, Nissen L, Gardner P, Heinone M, Hopia A, Huynh-Ba T, Lambelet P, McPail D, Skibsted L, Tijburg L. 2001. Investigation of plant extracts for the protection of processed foods against lipid oxidation. Comparison of antioxidant assays based on radical scavenging, lipid oxidation and analysis of the principal antioxidant compounds. Eur Food Res Technol 212: 319-328. https://doi.org/10.1007/s002170000256
  19. Brenes M, Gardia A, Gardia P, Rios J, Garrido A. 1999. Phenolic compounds in Spanish olive oils. J Agric Food Chem 47: 3535-3540. https://doi.org/10.1021/jf990009o
  20. Prior R, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53: 4290-4302. https://doi.org/10.1021/jf0502698
  21. Ho CT. 1992. Phenolic compounds in food and their effects on health I. ACS Symposium Series. Ho CT, Lee CY, Huang MT, eds. American Chemical Society, Washington, DC, USA. Vol 506, p 2-7. https://doi.org/10.1021/bk-1992-0506.ch001
  22. Van Sumere CF. 1989. Plant phenolics. In Methods in Plant Biochemistry. Harborne JB, ed. Academic Press, London, UK. Vol 1, p 29-73.
  23. Ezaki O, Takahashi M, Shigematsu T, Shimamura K, Kimura J, Ezaki H, Gotoh T. 1999. Long-term effects of dietary alpha-linolenic acid from perilla oil on serum fatty acid composition and on the risk factors of coronary heart disease in Japanese elderly subjects. J Nutr Sci Vitaminol 45: 759-772. https://doi.org/10.3177/jnsv.45.759
  24. Narisawa T, Fukaura Y, Yazawa K, Ishikawa C, Isoda Y, Nishizawa Y. 1994. Colon cancer prevention with a small amount of dietary perilla oil high in alpha linolenic acid in an animal model. Cancer 73: 2069-2075. https://doi.org/10.1002/1097-0142(19940415)73:8<2069::AID-CNCR2820730810>3.0.CO;2-1
  25. Okamoto M, Mitsunobu F, Ashida K, Mifune T, Hosaki Y, Tsugeno H, Harada S, Tanizaki Y, Kataoka M, Niiya K, Harada M. 2000. Effects of perilla seed oil supplementation on leukotriene generation by leucocytes in patients with asthma associated with lipometabolism. Int Arch Allergy Immunol 122: 137-142. https://doi.org/10.1159/000024369

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