Transactions of the Korean Society of Mechanical Engineers A (대한기계학회논문집A)

The Korean Society of Mechanical Engineers (대한기계학회)
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Development of Bioreactor for Regenerative Medicine and Effect of Mechanical Stimuli on Mesenchymal Stem Cells in Polyurethane Scaffolds

바이오리액터 개발과 기계적 자극에 의한 중간엽 줄기세포의 영향에 관한 연구

  • 주민진 (연세대학교 기계공학부) ;
  • 전흥재 (연세대학교 기계공학부) ;
  • 정형진 (연세대학교 기계공학부) ;
  • 이창근 (연세대학교 의과대학 의과학과) ;
  • 허동녕 (경희대학교 치의학대학원 구강생물학과) ;
  • 권일근 (경희대학교 치의학대학원 구강생물학과) ;
  • 문성환 (연세대학교 의과대학 의과학과)
  • Received : 2009.02.10
  • Accepted : 2010.03.23
  • Published : 2010.06.01
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Abstract

It is well known that mesenchymal stem cell(MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

중간엽 줄기세포(MSCs)는 미분화 상태의 세포로써, 섬유아세포, 연골아세포, 골아세포 등으로 분화하여 인체의 근골격계를 구성하며, 기계적 자극은 중간엽 줄기세포 분화를 결정하는 중요한 인자로 알려져 이다. 본 연구에서는, 세포가 생존하기 위한 환경을 제공하고, 세포가 기계적 자극조건에 따라 분화할 수 있도록 하는 바이오리엑터를 제안하엿다. 또한, 중간엽 줄기세포를 배양하기 위한 세포 지지체로써 PU(polyurethane)로 제작된 지지체를 제안하였다. 세포 분화를 확인하기 위하여, 중간엽 줄기세포를 PU 지지체에 seeding한 후, 바이오리엑터를 이용하여 기계적 자극에 의한 세포의 분화를 확인하였다.

Keywords

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