Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluations of Antioxidative Activity and Whitening Effect of Extracts from Different Parts of Cosmos bipinnatus

코스모스 부위별 추출물의 항산화 활성과 미백효능평가

  • Kim, Sun-Young (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic) ;
  • Lee, Min-Hye (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic) ;
  • Park, Soo-Nam (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Science and Technology, Research Center for Development of Green Cosmetic)
  • 김선영 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터) ;
  • 이민혜 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터) ;
  • 박수남 (서울과학기술대학교 자연생명과학대학 정밀화학과, 그린코스메틱연구개발센터)
  • Received : 2010.11.10
  • Accepted : 2010.12.20
  • Published : 2010.12.31
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Abstract

In this study, the antioxidative effect, cellular protective effect and inhibitory effect on tyrosinase of Cosmos bipinnatus extracts were investigated. The ethyl acetate fraction of Cosmos bipinnatus flower extract ($11.48\;{\mu}g$/mL) showed more excellent free radical (1,1-diphenyl-2-picrylhydrazyl radical, DPPH) scavenging activity (FSC50) than those of leaf and stem extracts ($17.45\;{\mu}g$/mL). Reactive oxygen species (ROS) scavenging activity (OSC50) of Cosmos bipinnatus extracts on ROS generated in $Fe^{3+}$-EDTA/H2O2 system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction of Cosmos bipinnatus flower extract ($0.56\;{\mu}g$/mL) showed 3 times more excellent ROS scavenging activity than L-ascorbic acid ($1.50\;{\mu}g$/mL). The protective effects of the ethyl acetate fractions of extracts from different parts of Cosmos bipinnatus on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fractions of leaf and stem extract and flower extracts suppressed photohemolysis in a concentration dependent manner ($10\sim50\;{\mu}g$/mL). The inhibitory effect of ethyl acetate fraction of Cosmos bipinnatus flower extract ($62.75\;{\mu}g$/mL) on tyrosinase was investigated to assess the whitening efficacy. The ethyl acetate fraction of Cosmos bipinnatus flower extract showed 3.5 times higher tyrosinase inhibitory effect than arbutin ($226.88\;{\mu}g$/mL) known as an effective whitening agent. These results indicate that fractions of Cosmos bipinnatus extracts can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O2$ and other ROS, and protect cellular membranes against ROS. Fractions of Cosmos bipinnatus extracts can be applicable to new functional cosmetics for antioxidant and whitening.

Keywords

References

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