Transactions of the Korean hydrogen and new energy society (한국수소및신에너지학회논문집)

The Korean Hydrogen and New Energy Society (한국수소및신에너지학회)
권호 표지

Effect of Light Intensity and Nitrogen Source on Hydrogen Production Using Rhodobacter sphaeroldes KD131

홍색 비유황 광합성 세균 Rhodobacter sphaeroldes KD131의 수소생산에 미치는 빛 세기 및 질소원의 영향

  • Jeon, Hyo-Jin (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Kim, Mi-Sun (Bioenergy Research Center, Korea Institute of Energy Research)
  • 전효진 (한국에너지기술연구원 바이오에너지연구센터) ;
  • 김미선 (한국에너지기술연구원 바이오에너지연구센터)
  • Published : 2010.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Photobiological hydrogen production using Rhodobacter sphaeroides KD131 was studied on the effect of light intensities and nitrogen sources. Media containing malate and glutamate were shown higher hydrogen production rate than that containing succinate and $(NH_4)_2SO_4$ at the $110\;W/m^2$ illumination by halogen lamp at $30^{\circ}C$. Media lacking glutamate as the nitrogen source exhibited higher hydrogen production than that containing glutamate. Initial cell concentration was optimized to 1.0 at the absorbance of 660 nm. Hydrogen production was increased by increasing the light intensity from 0 to $216\;W/m^2$ but the increasing rate declined over $108\;W/m^2$.

Keywords

References

  1. Harun Koku, Inci Eroglu, Ufuk Gunduz, Meral Yucel, Lemi Turker : "Aspect of the metabolism of hydrogen proouction by Rhoddaaer sphaeroideS', International Journal of Hydrogen Energy, Vol. 27, 2002, pp. 1315-1329. https://doi.org/10.1016/S0360-3199(02)00127-1
  2. Eui-Jin Kim, Ju-Sim Kim, Mi-Sun Kim, Jeong K. Lee : "Effect of changes in the level of light harvesting complexes of Rhodobacter sphaeroides on the photoheterotrophic production of hydrogen", International Journal of Hydrogen Energy, Vol. 31, 2006, pp. 531-538. https://doi.org/10.1016/j.ijhydene.2005.04.053
  3. Benemann J. : "Hydrogen biotechnology ; Progress and prospects", Natural publishing group(UK), New York, 1996, pp. 1101-1103.
  4. Nan-Qi Ren, Bing-Reng Liu, Guo-Xiang Zheng, De-Reng Xing, Xin Qhao, Wan-Qian Guo, Jie Ding : "Strategy for enhancing photo-hydrogen production yield by repeated fed-batch cultures", International Journal of hydrogen energy, Vol. 34, 2009, pp. 7579-7584. https://doi.org/10.1016/j.ijhydene.2009.07.030
  5. Basar Uyar, Inci Eroglu, Meral Yucel, Ufuk Gunduz, Lemi Turker : "Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors", International Journal of Hydrogen Energy, Vol. 32, 2007, pp. 4670-4677. https://doi.org/10.1016/j.ijhydene.2007.07.002
  6. 김미선, 문광웅, 이상근, 김선창: "Rhodopseudomonas sphaeroides에 의한 수소 생산", Kor. J. Appl. Microbiol. Biotechnol., Vol. 26, No.2, 1998, pp. 89-95.
  7. Matthew R Melnicki, Lucia Bianchi, Roberto De Philippis, Anastasios Ivlelis : "Hydrogen production during stationary phase in purple photosynthetic bacteria", International Journal of Hydrogen Energy, Vol. 33, 2008, pp. 6525-6534. https://doi.org/10.1016/j.ijhydene.2008.08.041
  8. 신종환, 박태현 : "생물학적 수소생산 공정", Korean Chem. Eng. Res., Vol. 44, No. 1, 2006, pp. 16-22.
  9. Mi-Sun Kim, Jin-Sook Baek, Jeong K. Lee: "Comparison of $H_{2}$ accumulation by Rhodobacter sphaeroides KD131 and its uptake hydrogenase and PHB synthase deficient mutant", International Journal of Hydrogen Energy, Vol. 31, 2009, pp. 121-127.