The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Antinociception Effect and Mechanisms of $Campanula$ $Punctata$ Extract in the Mouse

  • Park, Soo-Hyun (Department of Pharmacology, College of Medicine, Hallym University) ;
  • Sim, Yun-Beom (Department of Pharmacology, College of Medicine, Hallym University) ;
  • Lim, Soon-Sung (Department of Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Kim, Jin-Kyu (Department of Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Lee, Jin-Koo (Department of Institute of Natural Medicine, College of Medicine, Hallym University) ;
  • Suh, Hong-Won (Department of Pharmacology, College of Medicine, Hallym University)
  • Received : 2010.08.05
  • Accepted : 2010.09.14
  • Published : 2010.10.31
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Abstract

In the present study, the antinociceptive profiles of $Campanula$ $punctata$ extract were examined in ICR mice. The $Campanula$ $punctata$ contain a large dose of saponin. $Campanula$ $punctata$ extract administered orally (200 mg/kg) showed an antinociceptive effect as measured by the tail-flick and hot-plate tests. In addition, $Campanula$ $punctata$ extract attenuated the writhing numbers in the acetic acid-induced writhing test. Furthermore, the cumulative nociceptive response time for intrathecal (i.t.) injection of substance P ($0.7{\mu}g$) was diminished by $Campanula$ $punctata$ extract. Intraperitoneal (i.p.) pretreatment with yohimbine (${\alpha}_2$-adrenergic receptor antagonist) attenuated antinociceptive effect induced by $Campanula$ $punctata$ extract in the writhing test. However, naloxone (opioid receptor antagonist) or methysergide (5-HT serotonergic receptor antagonist) did not affect antinociception induced by $Campanula$ $punctata$ extract in the writhing test. Our results suggest that $Campanula$ $punctata$ extract shows an antinociceptive property in various pain models. Furthermore, this antinociceptive effect of $Campanula$ $punctata$ extract may be mediated by ${\alpha}_2$-adrenergic receptor, but not opioidergic and serotonergic receptors.

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