Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Enhancement of Skin Immune Activities of Spirulina maxima by High Pressure Extraction Process

Spirulina maxima 초고압 추출물의 피부 면역 활성 증진

  • Oh, Sung-Ho (Department of Biomaterials Engineering, College of Bioscience and Biotechnology Kangwon National University) ;
  • Kang, Do-Hyung (Marine Living Resources Research Department, KORDI) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology Kangwon National University) ;
  • Seo, Yong-Chang (Department of Biomaterials Engineering, College of Bioscience and Biotechnology Kangwon National University) ;
  • Heo, Soo-Jin (Marine Living Resources Research Department, KORDI) ;
  • Abu, Affan Md. (Marine Living Resources Research Department, KORDI) ;
  • Jeong, Kyung-Hwan (Department of Food Science & Technology, College of Health and Life Sciences Chungju University) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology Kangwon National University)
  • 오성호 (강원대학교 바이오산업공학부) ;
  • 강도형 (한국해양연구원 해양생물자원 연구부) ;
  • 최운용 (강원대학교 바이오산업공학부) ;
  • 서용창 (강원대학교 바이오산업공학부) ;
  • 허수진 (한국해양연구원 해양생물자원 연구부) ;
  • ;
  • 정경환 (충주대학교 보건생명대학 식품생명공학부) ;
  • 이현용 (강원대학교 바이오산업공학부)
  • Received : 2010.04.26
  • Accepted : 2010.06.15
  • Published : 2010.06.30
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Abstract

A marine alga, Spirulina maxima, was extracted under high pressure and low temperature conditions at 500 MPa and $60^{\circ}C$ for 5 and 10 min. A high pressure of 500 MPa was applied to improve process yields because of low temperature extraction. This method resulted in highest higher extraction yield of 26.1% (w/w) in comparison to those results obtained from conventional extraction methods which produced a yield of 17.6% (w/w) from water. The extracts from this process also showed 19% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. The crude extract significantly reduced the production of Prostaglandin $E_2$ ($PGE_2$) from CCD-986sk cells and increased nitric oxide production by macrophages. These higher activities of enhancing skin immune functions were found to have high antioxidant extract properties, like a 98% increase in DPPH radical scavenging activity. The extracts from the high pressure process showed a higher elution of active components than other processes and generated new compounds based on HPLC analysis. This clearly indicates that the extracts from high pressure and low temperature conditions have higher skin immune activation properties that have not been previously reported.

Keywords

References

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