DOI QR코드

DOI QR Code

Proteomics-Driven Identification of SCO4677-Dependent Proteins in Streptomyces lividans and Streptomyces coelicolor

  • Choi, Si-Sun (Department of Biological Engineering, Inha University) ;
  • Kim, Seon-Hye (Department of Biological Engineering, Inha University) ;
  • Kim, Eung-Soo (Department of Biological Engineering, Inha University)
  • Received : 2009.10.05
  • Accepted : 2009.11.06
  • Published : 2010.03.31

Abstract

AfsR2 is a global regulatory protein that stimulates antibiotic biosynthesis in both Streptomyces lividans and S. coelicolor. Previously, various afsR2-dependent genes including a putative abaA-like regulatory gene, SCO4677, were identified through comparative DNA microarray analysis. To further identify the putative SCO4677-dependent proteins, the comparative proteomics-driven approach was applied to the SCO4677-overexpressing strains of S. lividans and S. coelicolor along with the wild-type strains. The 2D gel electrophoresis gave approximately 277 protein spots for S. lividans and 207 protein spots for S. coelicolor, showing different protein expression patterns between the SCO4677-overexpressing strains and the wild-type strains. Further MALDI-TOF analysis revealed that only 18 proteins exhibited similar expression patterns in both S. lividans and S. coelicolor, suggesting that the SCO4677 could encode an abaA-like regulator that controls a few cross-species common proteins as well as many species-specific proteins in Streptomyces species.

Keywords

References

  1. Chater, K. F. 1990. Multilevel regulation of Streptomyces differentiation. Trends Genet. 5: 372-377.
  2. Champness, W. C., P. Riggle, T. Adamidis, B. Kenney, and D. Aceti. 1993. Genetic elements involved in global antibiotic regulation in Streptomyces coelicolor, pp. 227-233. In R. Baltz et al. (ed.). Industrial Microorganisms: Basic and Applied Molecular Genetics. American Society for Microbiology, Washington DC, U.S.A.
  3. Champness, W. C. and K. F. Chater. 1994. Regulation and integration of antibiotic production and morphological differentiation in Streptomyces spp., pp. 61-93. In P. J. Piggot, C. P. Moran Jr., and P. Youngman (eds.). Regulation of Bacterial Differentiation. American Society for Microbiology, Washington DC, U.S.A.
  4. Fernandez-Moreno, M. A., A. J. Martin-Triana, E. Martinez, J. Niemi, H. M. Kieser, D. A. Hopwood, and F. Malpartida. 1992. abaA, a new pleiotropic regulatory locus for antibiotic production in Streptomyces coelicolor. J. Bacteriol. 174: 2958-2967.
  5. Hara, O., S. Horinouchi, T. Uozumi, and T. Beppu. 1983. Genetic analysis of A-factor synthesis in Streptomyces coelicolor A3(2) and Streptomyces griseus. J. Gen. Microbiol. 129: 2939-2944.
  6. Hopwood, D. A. 1988. Towards an understanding of gene switching in Streptomyces, the basis of sporulation and antibiotic production. Proc. R. Soc. Lond. Series B 235: 121-138.
  7. Horinouchi, S., O. Hara, and T. Beppu. 1983. Cloning of a pleiotropic gene that positively controls biosynthesis of Afactor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans. J. Bacteriol. 155: 1238-1248.
  8. Horinouchi, S., M. Kito, M. Nishiyama, K. Furuya, S. K. Hong, K. Miyake, and T. Beppu. 1992. Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2). Gene 95: 49-56.
  9. Kim, C. Y., J. H. Noh, H. N. Lee, and E.-S. Kim. 2009. Functional analysis of an antibiotic regulatory gene, afsR2 in S. lividans, through DNA microarray system. Korean J. Biotechnol. Bioeng. 24: 259-266.
  10. Kim, C. Y., H. J. Park, and E.-S. Kim. 2005. Proteomics-driven identification of putative AfsR2-target proteins stimulating antibiotic biosynthesis in Streptomyces lividans. Biotechnol. Bioproc. Eng. 10: 248-253. https://doi.org/10.1007/BF02932021
  11. Kim, C. Y., H. J. Park, and E.-S. Kim. 2006. Functional dissection of sigma-like domain in antibiotic regulatory gene, afsR2 in Streptomyces lividans. J. Microbiol. Biotechnol. 16: 1447-1480.
  12. Kim, C. Y., H. J. Park, Y. J. Yoon, H. Kang, and E.-S. Kim. 2004. Stimulation of actinorhodin production by Streptomyces lividans with a chromosomally-integrated antibiotic regulatory gene afsR2. J. Microbiol. Biotechnol. 14: 1089-1092.
  13. Kim, J. H., J. S. Lim, and S. W. Kim. 2004. The improvement of cephalosporin C production by fed-batch culture of Cephalosporium acremonium M25 using rice oil. Biotechnol. Bioproc. Eng. 9: 459-464. https://doi.org/10.1007/BF02933486
  14. Kim, E.-S., H.-J. Hong, C.-Y. Choi, and S. N. Cohen. 2001. Modulation of actinorhodin biosynthesis in Streptomyces lividans by glucose repression of afsR2 gene transcription. J. Bacteriol. 183: 2198-2203. https://doi.org/10.1128/JB.183.7.2198-2203.2001
  15. Kormanec, J., A. Lempel'ova, R. Novakova, B. Rezuchova, and D. Homerova. 1997. Expression of the Streptomyces aureofaciens glyceraldehyde-3-phosphate dehydrogenase gene (gap) is developmentally regulated and induced by glucose. Microbiology 143: 3555-3561. https://doi.org/10.1099/00221287-143-11-3555
  16. Lee, K. J. and J. Y. Lim. 2004. Optimized conditions for high erythritol production by Penicillium sp. KJ-UV29, mutant of Penicillium sp. KJ81. Biotechnol. Bioproc. Eng. 8: 173-178.
  17. Matsumoto, A., H. Ishizukz, T. Beppu, and S. Horinouchi. 1995. Involvement of a small ORF downstream of the afsR gene in the regulation of secondary metabolism in Streptomyces coelicolor A3(2). Actinomycetologica 9: 37-43. https://doi.org/10.3209/saj.9_37
  18. Nakamura, Y., C. Asada, and T. Sawada. 2004. Production of antibacterial violet pigment by psychrotropic bacterium RT102 strain. Biotechnol. Bioproc. Eng. 8: 37-40.
  19. Oakley, B. R., D. R. Kirsch, and N. R. Morris. 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal. Biochem. 105: 361-363. https://doi.org/10.1016/0003-2697(80)90470-4
  20. Shevchenko, A., M. Wilm, O. Vorm, and M. Mann. 1996. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 68: 850-858. https://doi.org/10.1021/ac950914h
  21. Sohlberg, B., J. Huang, and S. N. Cohen. 2003. The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA. J. Bacteriol. 185: 7273-7278. https://doi.org/10.1128/JB.185.24.7273-7278.2003
  22. Sprusansky, O., B. Rezuchova, D. Homerova, and J. Kormanec. 2001. Expression of the gap gene encoding glyceraldehyde-3-phosphate dehydrogenase of Streptomyces aureofaciens requires GapR, a member of the AraC/XylS family of transcriptional activators. Microbiology 147: 1291-1301.
  23. Stein, D. and S. N. Cohen. 1989. A cloned regulatory gene of Streptomyces lividans can suppress the pigment deficiency phenotype of different development mutants. J. Bacteriol. 171: 2258-2261.
  24. Vogtli, M., P. C. Chang, and S. N. Cohen. 1994. afsR2: A previously undeleted gene encoding a 63-amino-acid protein that stimulates antibiotic production in Streptomyces lividans. Mol. Microbiol. 14: 643-653. https://doi.org/10.1111/j.1365-2958.1994.tb01303.x