Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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ITS-PCR Analysis for the Discrimination of Moutan Cortex

목단피의 감별을 위한 ITS-PCR 분석

  • 이재웅 (충남대학교 농업생명과학대학 농학과) ;
  • 김영화 (한국한의학연구원) ;
  • 고병섭 (한국한의학연구원) ;
  • 육진아 (한국한의학연구원) ;
  • 오승은 (건국대학교 이과대학 생명과학과) ;
  • 박상언 (충남대학교 농업생명과학대학 농학과) ;
  • 이미영 (한국한의학연구원)
  • Received : 2010.01.08
  • Accepted : 2010.02.11
  • Published : 2010.02.28
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Abstract

The application of PCR analysis on the herbal medicine Moutan Cortex (Paeonia suffruticosa Andrews) was evaluated by the comparison of the genetic relationship based on the DNA sequence with Paeoniae Radix (Paeonia lactiflora Pallas) following development of specific primers. Moutan Cortex and Paeoniae Radix were distinguished through the PCR analysis based on the internal transcribed spacer (ITS-PCR) from nuclear ribosomal DNA region. The 294 bp PCR products both of Moutan Cortex and Paeoniae Radix was amplified by MIF1 and MIR1. And a Moutan Cortex specific 225 bp PCR amplification product was amplified by MIF2 and MIR1 primers. The 225 bp sequence could be successfully amplified from Mortan Cortex of dried herbal preparations. PCR analysis based on ITS (ITS-PCR) may be an efficient tool for the discrimination of Moutan Cortex.

Keywords

References

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