The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory effect and contents from the aerial part and root of the various Taraxacum spp. distributed in Korea

국산 5종 포공영(蒲公英)의 항염 효과 및 성분 함량 비교 연구

  • Lee, Mi-Hwa (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Song, Sun-Ho (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Ham, In-Hye (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Bu, Young-Min (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ho-Cheol (Department of Herbology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, Kyung Hee University)
  • 이미화 (경희대학교 한의과대학 본초학교실) ;
  • 송선호 (경희대학교 한의과대학 본초학교실) ;
  • 함인혜 (경희대학교 한의과대학 본초학교실) ;
  • 부영민 (경희대학교 한의과대학 본초학교실) ;
  • 김호철 (경희대학교 한의과대학 본초학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실)
  • Received : 2010.11.07
  • Accepted : 2010.12.15
  • Published : 2010.12.30
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Abstract

Objectives : Taraxaci Herba et Radix (THR) is widely used as a food and medicinal herb in Korea. It has been used for treatment of virus inflammatory disease, liver diseases and gastritis. So far, anti-inflammatory effects and constituents of various species in THR has not been studied for comparison. The aim of this study is to compare the anti-inflammatory effects of the aerial part and root from various THR. Also, we have compared the contents of its known constituents with each. Methods : In this study, we estimated anti-inflammatory effect and compared their constituent by HPLC. For the determination of anti-inflammatory effects, we investigated NO and $PGE_2$ production by ELISA. The expressions of iNOS was determined by western blotting in LPS-induced RAW 264.7 macrophage cells. And, standard compounds which are methyl gallate, gallic acid, syringic acid and esculetin of THR were analyzed by HPLC using a $C_{18}$ column. Results : Methanol extracts of THR decreased NO and $PGE_2$ production. The expressions of iNOS protein were also decreased in methanol extracts of THR. As a result, HPLC analysis showed that they showed similar patterns. Methyl gallate and esculetin showed the highest content. Methyl gallate was included over 10% content in each aerial part and root of THR. Conclusions : These results indicate that most of THR distributed in Korea might represent therapeutic agent for treatment of inflammatory diseases.

Keywords

References

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