Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison of Biological Activities of Extracts from Different Parts of Seabuckthorn (Hippophae rhamnoides L.)

비타민나무(Seabuckthorn, Hippophae rhamnoides L.) 부위별 추출물의 생리활성 비교

  • Park, Yu-Hwa (Gangwon Provincial Agricultural Research & Extention Services) ;
  • Lim, Sang-Hyun (Gangwon Provincial Agricultural Research & Extention Services) ;
  • Ham, Hun-Ju (Gangwon Provincial Agricultural Research & Extention Services) ;
  • Jeong, Haet-Nim (Ginseng & Medicinal Plants Experiment Station) ;
  • Lee, Kwang-Jae (Gangwon Provincial Agricultural Research & Extention Services) ;
  • Kim, Kyung-Hee (Gangwon Provincial Agricultural Research & Extention Services) ;
  • Kim, Song-Mun (Dept. of Biological Environment, Kangwon National University)
  • 박유화 (강원도 농업기술원 농산물이용시험장) ;
  • 임상현 (강원도 농업기술원 농산물이용시험장) ;
  • 함헌주 (강원도 농업기술원 농산물이용시험장) ;
  • 정햇님 (강원도 농업기술원 인삼약초시험장) ;
  • 이광재 (강원도 농업기술원 농산물이용시험장) ;
  • 김경희 (강원도 농업기술원 농산물이용시험장) ;
  • 김성문 (강원대학교 바이오자원환경학과)
  • Received : 2010.03.30
  • Accepted : 2010.05.07
  • Published : 2010.07.31
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Abstract

Biological activities of different parts (stems, leaves, roots, fruits) and solvents (water, ethanol) of seabuckthorn (Hippophae rhamnoides L.) grown in Korea were tested as follows. In the experiment of inhibiting $\alpha$-glucosidase activity, ethanol extract of Hippophae rhamnoides L. stem showed the highest inhibitory activity by 93% and the next highest was the ethanol extract of its leaf by 88.7%. In the case of these two extracts, the effect of inhibiting $\alpha$-glucosidase activity was extraordinarily great when comparing with control group, acarbose. In the experiment of inhibiting $\alpha$-amylase activity, water extract of leaf showed the highest result by 54.7%, among all extracts. Regarding anticancer effect for HT-29 cell and DU-145 cell, water extract of root showed 47.1% and 32.3% activities, respectively. The experiment on antibacterial activity showed that the ethanol extract from the leaf inhibitory activity of Clostridium butyricum, Proteus mirabilis, and Shigella flexneri which are the several food borne pathogenic strains. In future research, materials for biological activity appear isolated and purified and research should continue.

국내에서 재배된 비타민나무(Hippophae rhamnoides L.)의 생리활성을 부위별(줄기, 잎, 뿌리, 열매), 용매별(물, 에탄올)로 시험한 결과는 다음과 같다. $\alpha$-Glucosidase 활성 억제 실험에서, 비타민나무의 저해활성은 줄기의 에탄올 추출물이 93%로 가장 높았고, 다음이 잎의 에탄올 추출물이 88.7% 순으로서 대조구 acarbose의 20.2%보다 훨씬 높았다. Pancreatin 기원의 $\alpha$-amylase 억제 활성 측정 실험에서는 잎의 물 추출물이 54.7%로 다른 추출물에 비해 가장 높았다. HT-29 cell과 DU-145 cell의 암세포에 대한 항암효과 시험 결과 뿌리의 물 추출물에서 각각 47.1%, 32.3%의 항암효과가 나타났다. 또한 항균활성 실험 결과 잎의 에탄올 추출물에서 위해균주인 Clostridium butyricum, Proteus mirabilis, Shigella flexneri에 대한 저해활성이 나타났다. 향후 연구에서는 생리활성 효과가 나타난 물질에 대한 분리, 정제 등의 연구가 계속 수행되어져야할 것으로 생각된다.

Keywords

References

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