Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Perilla frutescens Extract on Anti-allergic Reactions in a Mouse Model

소엽 추출물이 마우스모델에서 항알레르기 반응에 미치는 영향

  • Ko, Jung-A (School of Life Sciences and Biotechnology, Korea University) ;
  • Lim, Hun-Sun (Department of Bio R&D, Korea Medi Co. Ltd.) ;
  • Kim, Gun-Hee (Department of Food and Nutrition, DukSung Women's University) ;
  • Park, Ji-Yong (Department of Biotechnology, Yonsei University) ;
  • Han, Chan-Kyu (Korea Food Research Institute) ;
  • Park, Hyun-Jin (School of Life Sciences and Biotechnology, Korea University)
  • Received : 2010.03.26
  • Accepted : 2010.04.23
  • Published : 2010.08.31
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Abstract

We investigated the effect of Perilla frutescens (PF) ethanol extract powder (PF-E30) on the local allergic reaction activated by anti-DNP IgE and the mast cell-mediated immediate-type allergic reactions induced by compound 48/80 in a mouse model. One gram of PF powder extracted with 30% ethanol at $80^{\circ}C$ contained 12.3 mg of rosmarinic acid. Oral administration of PF-E30 (0.1 to 0.5 mg/kg body weight) significantly reduced plasma histamine levels and inhibited histamine release from peritoneal mast cells in mice activated by compound 48/80 or anti-DNP IgE. Moreover PF-E30 dose-dependently inhibited the production of antigen-induced IgE. These results indicate that the PF ethanol extract inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.

$80^{\circ}C$에서 30% 주정으로 추출된 소엽추출물 분말의 로즈마린산 함량은 12.3 mg/g이었으며, 소엽 30% 주정추출물분말(PF-E30)은 생쥐모델 실험에서 ant-DNP IgE으로 활성화된 local allergy 반응과 compound 48/80으로 유도된 mast cell-mediated immediatetype allergy 반응에 대한 억제 효과를 나타내었다. 더욱이, PFE30(0.1-0.5 mg/kg BW)의 투여는 혈장 히스타민 수준을 유의성있게 감소시켰으며, compound 48/80 또는 anti-DNP IgE으로 활성화된 복막 비만세포로부터 히스타민 방출을 억제하였다. 특히 PFE30는 antigen-induced IgE 의 생산을 농도 의존적으로 억제하였다. 이런 결과는 소엽 주정추출물이 in vivo 및 in vitro 실험에서 mast cell-mediated immediate-type allergy 반응을 저해한다는 것을 제시한다. 소엽 주정추출물이 알레르기 반응을 억제하는 기능성 식품 소재로서의 개발을 위해서는 mast cell mediated-type allergy 반응에 대한 보다 많은 연구가 필요할 것이다.

Keywords

References

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