A Study of the Inhibitory Effect of Acer tegmentosum Max. on Fibrogenesis in Hepatic Stellate Cell Line T6

산청목(山靑木)이 간섬유화 진행 억제에 미치는 효과에 대한 연구

  • Lee, Seung-Bo (Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Woo, Hong-Jung (Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 이승보 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2010.06.30


Objectives : This study was performed in order to investigate the anti-fibrogenic effect of Acer tegmentosum Maxim. on r at hepatic stellate cell line T6. Materials and Methods : Hepatic stellate Cells (T6) were treated with various concentrations of distilled water Acer teg mentosum Maxim. extract for 24, 48, 72 hours. After the treatment, cell viability, proliferation, procollagen levels, mRNA of AS MA, MMP-2, collagen type 1a2 and IL-6 production were measured using MTT assay, BrdU assay, RT-PCR, procollagen typ e 1 C-peptide EIA kit and murine IL-6 ELISA development kit. Results : Cell viability of HSC-T6 decreased significantly in both 24 hours and 48 hours groups in a dose-dependant man ner. Proliferation of HSC also decreased in the same way. In the RT-PCR, mRNA expression of collagen type 1a2 and ASMA decreased in the groups which were treated with Acer tegmentosum Maxim. for 24 hours. The production of procollagen tended to decrease in a dose-dependant manner in the 24 hours treated group. IL-6 production increased under Acer tegmentosum trea tment in a dose-dependant manner in both 24 and 48 hours groups. Conclusion : These results show the possibility that Acer tegmentosum Maxim. can be an effective remedy for liver fibrosi s and liver cirrhosis.


  1. 통계청. 사망원인통계. 2008.
  2. Jun-Sung Lee, Jong-Hoon Kim. The Role of Activated Hepatic Stellate Cells in Liver Fibrosis, Portal Hypertension and Cancer Angiogenesis. The Korean Journal of Hepatology. 2007;13: 309-19. https://doi.org/10.3350/kjhep.2007.13.3.309
  3. 최은경, 이장훈, 김영철, 우홍정. 단삼이 간성상세포의 섬유화 억제에 미치는 영향. 대한한방내과학회지. 2008;29(2):299-310.
  4. 김성아, 우홍정, 김영철, 이장훈. 인진이 간성상세포의 섬유화 억제에 미치는 영향. 대한한방내과학회지. 2008;29(1):177-88.
  5. 김세훈, 우홍정, 이장훈, 김영철. 울금이 간성상세포의 섬유화 억제에 미치는 영향. 대한한방내과학회지. 2009;30(2):306-16.
  6. 이홍일, 김영철, 우홍정, 이장훈. 인진청간탕이 간성상세포의 섬유화 억제에 미치는 영향. 대한한방내과학회지. 2009;30(1):74-84.
  7. Bo-Kyong Hong, Seok-Hyun Eom, Chan-Ok Lee, Ji-Won Lee, Jong-Hyun Jeong, Jae-Kwang Kim, Dong-Ha Cho, Chang-Yeon Yu, Yong-Soo Kwon, Myong-Jo Kim. Biological Activities and Bioactive Compounds in the Extract of Acer tegmentosum Maxim. Stem. Korean J. Medicinal Crop Sci. 2007;15(4):296-303.
  8. In-Cheol Shin, Jae-Hoon Sa, Tae-Heum Shim, Jin-ha Lee. The Physical and Chemical Properties and Cytotoxic Effects of Acer tegmentosum Maxim. Extracts. J. Korean Soc. Appl. Biol. Chem. 2006;49(4):322-7.
  9. Sung-Hoon Kim, Hee-Juhn Park, Jong-Won Choi. Hepatoprotective Activity of Salidroside from Acer Tegmentosum Max on D-galactosamine Induced Hepatotoxicity in Rats. Korean J. Oriental Physiology & Pathology. 2008;22(6): 1525-31.
  10. Ha-Na Kown, Jyung-Rewng Park, Jeong-Ryae Jeon. Antioxidative and Hepatoprotective Effects of Acer tegmentosum M. Extracts. J Korean Soc Food Sci Nutr. 2008;37(11):1389-94. https://doi.org/10.3746/jkfn.2008.37.11.1389
  11. Friedman SL. Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. J Biol Chem. 2000;275:2247-50. https://doi.org/10.1074/jbc.275.4.2247
  12. Young-Han Paik. Fibrogenesis and antifibrotic therapy. The Korean Journal of Hepatology. 2008;14(2s):7-13.
  13. Hasegawa T, Yoneda M, Nakamura K, Makino I, Terano A. Plasma transforming growth factor -beta1 level and efficacy of alpha-tocopherol in patients with non-alcoholic steatohepatitis: a pilot study. Aliment Pharmacol Ther. 2001; 15:1667-72. https://doi.org/10.1046/j.1365-2036.2001.01083.x
  14. Lindor KD, Kowdley KV, Heathcote EJ, Harrison ME, Jorgensen R, Angulo P, et al. Ursodeoxycholic acid for treatment of nonalcoholic steatohepatitis: results of a randomized trial. Hepatology. 2004; 39:770-8. https://doi.org/10.1002/hep.20092
  15. Yokohama S, Yoneda M, Haneda M, Okamoto S, Okada M, Aso K, et al. Therapeutic efficacy of an angiotensin.
  16. 안덕균. 한국본초도감. 교학사; 1998, p. 523.
  17. 김일훈. 神藥. 인산동천; 1986. p. 78-9.
  18. Jong-Moon Hur, Eun-Ju Yang, Sun-Ha Choi, Kyung-Sik Song. Isolation of Phenolic Glucosides from the Stem of Acer tegmentosum Max. J. Korean Soc. Appl. Biol. Chem. 2006;49(2): 149-52.
  19. Jong-Moon Hur, Mira Jun, Eun-Ju Yang, Sun-Ha Choi, Jong-Cheol Park, Kyung Sik Song. Isolation of Isoprenoidal Compounds from the Stem of Acer tegmentosum Max. Kor. J. Pharmacogn. 2007;38(1):67-70.
  20. Sung-Hoon Kim, Hee-Juhn Park, Jong-Won Choi. Protective Mechanism of Salidroside Isolated from Acer tegmentosum Max on Cyclosporine -Induced Nephrotoxicity in Rats. Korean J. Oriental Physiology & Pathology. 2009;23(1): 166-173.
  21. Xiao-Dong Peng, Li-Li Dai, Chang-Quan Huang, Chun-Mei He, Li-Juan Chen. Correlation between anti-fibrotic effect of baicalin and serum cytokines in rat hepatic fibrosis. World J Gastroenterol. 2009;15(37):4720-5. https://doi.org/10.3748/wjg.15.4720
  22. Nathalie Franchimont, Sheila Rydziel, Anne M. Delany and Ernesto Canalis. Interleukin-6 and Its Soluble Receptor Cause a Marked Induction of Collagenase 3 Expression in Rat Osteoblast Cultures. J Biol Chem. 1997;272(18): 12144-50. https://doi.org/10.1074/jbc.272.18.12144
  23. Kenichiro Kusano, Chisato Miyaura, Masaki Inada, Tatsuya Tamura, Akira Ito, Hideaki Nagase, Kyuichi Kamoi and Tatsuo Suda. Regulation of Matrix Metalloproteinases (MMP-2, -3, -9, and -13) by Interleukin-1 and Interleukin -6 in Mouse Calvaria: Association of MMP Induction with Bone Resorption. Endocrinology. 1998;139(3):1338-45. https://doi.org/10.1210/en.139.3.1338
  24. Solis-Herruzo JA, Rippe RA, Schrum LW, de La Torre P, Garcia I, Jeffrey JJ, Munoz-Yague T, Brenner DA. Interleukin-6 increases rat metalloproteinase-13 gene expression through stimulation of activator protein 1 transcription factor in cultured fibroblasts. J Biol Chem. 1999;274(43):30919-26. https://doi.org/10.1074/jbc.274.43.30919
  25. J. A. Solis Herruzo, P. de la Torre, T. Diaz Sanjuan, I. Garcia Ruiz and T. Munoz Yague. IL-6 and extracellular matrix remodelling. Rev Esp Enferm Dig. 2005;97(8):575-95.