Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Antioxidant Activity and Protective Effects of 9-hydroxy-$\alpha$-tocopherone from Viola mandshurica Extracts

제비꽃(Viola mandshurica) 추출물로부터 분리된 9-hydroxy-$\alpha$-tocopherone의 항산화 활성 및 세포 보호효과

  • Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Ji-Hwan (National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Park, Jae-Hee (Department of Food and Nutrition, Kyungnam University) ;
  • Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Eun-Ju (Department of Food and Nutrition, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 이미라 (경남대학교 식품생명학과) ;
  • 황지환 (일본 산업기술종합연구소) ;
  • 박재희 (경남대학교 식품영양학과) ;
  • 김현정 (경남대학교 식품생명학과) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Received : 2010.07.15
  • Accepted : 2010.09.08
  • Published : 2010.09.30
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Abstract

Oxidative stress to proteins, lipids, or DNA is higher in human autopsy tissue and in rodent models of a number of neurodegenerative conditions, including Alzheimer's and Parkinson's disease. On the basis of this information, we established a screening system using N18-RE-105 cells to identify therapeutic agents that can protect cells from glutamate toxicity. During the course of our screening program, we recently isolated the active compound 9-hydroxy-$\alpha$-tocopherone ($\alpha$-TP), which prevents glutamate-induced cell death, from Viola mandshurica. The chemical structure of $\alpha$-TP was identified using spectroscopic methods and by comparison with literature values. Antioxidant activity and protective effects of $\alpha$-TP were evaluated by DPPH radical-scavenging assay, morphological assay, MTT reduction assay, and lactate dehydrogenase (LDH) release assay. These results suggest that $\alpha$-TP could be a new potential chemotherapeutic agent against neuronal diseases.

Keywords

References

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