Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Anti-stress Effect of Lactic Acid Bacteria (Lactobacillus acidophilus, Pediococcus pentosaseus, Bifidobacterium longum SPM1205)

유산균 (Lactobacillus acidophilus, Pediococcus pentosaseus, Bifidobacterium longum SPM1205)의 항스트레스 효과

  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

이 연구의 목적은 유산균(LAB)의 항스트레스 효과를 알아보기 위함이다. 본 실험에서는 ICR 마우스에게 구속 스트레스를 가하면서 혼합유산균과 홍삼추출물을 경구투여 하였다. 5일간 Normal 그룹을 제외한 saline (control), LAB100, LAB200, RGE200에게 구속 스트레스를 주었다. 구속 스트레스를 가한 후에 ICR 마우스에게 kg당 100 mg ($3.0\times10^{11}$ CFU/g) 또는 200 mg $3.0\times10^{11}$ CFU/g)의 혼합유산균(Lactobacillus acidophilus, Pediococcus pentosaseus, Bifidobacterium longum SPM1205)을 투여하였으며 대조물질로는 홍삼 추출물(Red ginseng extract) 200 mg (홍삼추출물)/kg (마우스)을 투여하였다. 마지막 실험일에는 locomotor와 elevated plus-maze 실험을 통하여 마우스의 행동변화를 측정하였다. 그 결과, 혼합유산균을 투여한 그룹에서는 스트레스를 일부 억제하는 효과를 보였다. 특히 Elevated plus-maze 실험에서, 스트레스를 받은 마우스는 open zone에서보다 closed zone에서 더욱 많은 시간을 보냈다. 그렇지만 혼합유산균을 투여한 그룹에서는 open zone에서 더욱 오래 시간을 보냈으며 그 시간은 saline과 RGE200 그룹에 비해서도 길었다. 그것은 또한 아무것도 처리하지 않은 Normal 그룹과도 비슷한 결과이다. 그리고 마우스의 분변에서 유산균수를 측정하였는데 스트레스를 가하고 혼합유산균은 투여하지 않은 saline 그룹에서는 유산균수가 감소했지만 혼합유산균을 투여한 LAB100, LAB200 그룹에서는 유산균수가 증가하였다.

Keywords

References

  1. Araya M, Morelli L, Reid G, Sanders ME, Stanton C, Pineiro M and Ben Embarek P. Guidelines for the evaluation of probiotics in food, Joint FAO/WHO working group report on drafting guidelines for the evaluation of probiotics in food, London, On, Canada, 2002.
  2. Breier A, Albus M, Picker D, Zahn TP, Wolkowitz OM and Paul SM. Controllable and uncontrollable stress in humans: Alterations in mood and neuroendocrine and psychophysiological function, AM J Psychiatry 1987; 144: 11-19.
  3. Choi SS, Lee JK and Suh HW. Effect of Ginsenosides administered intrathecally on the antinocieption induced by cold water swimming stress in the mouse, Boil Pharm Bull 2003; 26: 858-861. https://doi.org/10.1248/bpb.26.858
  4. Chrousos GP and Gold PW. The concepts of stress and stress system disorders, Overview of physical and behavioral homeostasis, JAMA 1992; 267: 1244-1252. https://doi.org/10.1001/jama.267.9.1244
  5. Dimsdale JE, Keefe HJ and Stein MB. Stress and psychiatry, Comprehensive textbook of psychiatry, Sadock BJ, Sadock VA (eds), Philadelphia, PA, USA 2000; 2: 1835-1846.
  6. Djordjevic J, Cvijic G and Davidovic V. Different activation of ACTH and corticosterone release in response to various Stressors in rat, Physiol Res 2003; 52: 67-72.
  7. Fanselow MS. Conditional and unconditional components of post-shock freezing, Pavlov J Viol Sci 1980; 15: 177-182.
  8. Glass DC. Stress, behavior patterns, and coronary disease, Am Scientist 1977; 65: 177-187.
  9. Hogg S. A review of the validity and variability of the elevated plus-maze as an animal model of anxiety, Pharmacol Biochem Behav 1996; 54: 21-30. https://doi.org/10.1016/0091-3057(95)02126-4
  10. Hurst MW, Jenkins CD and Rose RM. The relation of psychological stress to onset of medical illness, Ann Rev Med 1976; 27: 301-312. https://doi.org/10.1146/annurev.me.27.020176.001505
  11. Kaneko H and Nakanishi K. Proof of the mysterious efficacy of Ginseng: basic and clinical trials: clinical effects of medical Ginseng, Korean Red Ginseng: specifically, its anti stress action for prevention of disease, J Pharmacol Sci 2004; 95: 158-162. https://doi.org/10.1254/jphs.FMJ04001X5
  12. Kaneko H, Nakanishi K, Murakami A, Kaidoh H and Kuwashima K. The acute effects of massive dose of red ginseng on healthy adults under the condition of cold stress, Ginseng Rev 1996; 22: 20-24.
  13. Kim C, Kim CC, Kim MS, Hu CY and Rhe JS. Influence of ginseng on the stress mechanism, Lioydia 1970; 33: 43-48.
  14. Kim DH, Jung JS, Moon YS, Sung JH, Suh HW, Kim YH and Song DK. Inhibition of intracerbroventricular injection stress-induced plasma corticosterone levels by intracerebroventricularly administered compound K, a Ginseng saponin metabolite, in mice, Biol Pharm Bull 2003a; 26: 1035-1038. https://doi.org/10.1248/bpb.26.1035
  15. Kim DH, Moon YS, Jung JS, Min SK, Son BK, Suh HW and Song DK. Effects of Ginseng saponin administered intraperitoneally on the hypothalamo-pituitary-adrenal axis in mice, Neurosci Lett 2003b; 343: 62-66. https://doi.org/10.1016/S0304-3940(03)00300-8
  16. Lee J, Amentani A, Enomoto A, Sato Y, Motoshima H, Ike F and Kaminogawa S. Screening for the immunopotentiating activity of food microorganism and enhancement of the immune response by Bifidobacterium adolescentis M1014, Siosci Biotech Biochem 1993; 57: 2127. https://doi.org/10.1271/bbb.57.2127
  17. Morimoto A, Nakamori T, Morimoto K, Tan N and Murakami N. The central role of corticotrophin-releasing factor (CRF-41) in psychological stress in rats, J Physiol 1993; 460: 221-229. https://doi.org/10.1113/jphysiol.1993.sp019468
  18. Noldus LPJJ, Spink AJ and Tegelenbosch AJ. EthoVision: A versatile video tracking system for automation of behavioral experiments, Psychonomic Society 2001; 33: 398-414.
  19. Oh CH, Kang PS, Vim JW, Kwon J and Oh SH. Water extracts of cultured mountain ginseng stimulate immune cells and inhibit cancer cell proliferation, Food Science and Biotechnology 2006; 15: 369-373.
  20. Perdigon G, de Macias ME, Alvarez S, Oliver G and de Ruiz Holgado AA. Effect of perorally administered lactobacilli on macrophage activation in mice, Infect Immun 1986; 53: 404.
  21. Selye H. History of the stress concept, In: Handbook of Stress, Ed by Goldberg L. Brenitz S. New York, USA The Free Press 1993; 7-36.
  22. Simsek N, Karadeniz A and Karaca T. Effects of the Spirulina platensis and Panax ginseng oral supplementation on peripheral blood cell in rats, Rev Med Vet 2007; 158: 483-488.
  23. Takahashi M, Tokuyama S and Kaneto H. Anti-stress effect of ginseng on the inhibition of the development of morphine tolerance in stressed mice, Jpn J Pharmacol 1992; 59: 399-404. https://doi.org/10.1254/jjp.59.399
  24. Takeuchi T, Iwanaga M and Harada E. Possible regulatory mechanism of DHA-induced anti-stress reaction in rats, Brain Res 2003; 964: 136-143. https://doi.org/10.1016/S0006-8993(02)04113-6
  25. Tan-Lee BS, Yu GY, Kim K, Han H, Han J, Lee GS, Kim ES, Lee CJ, Ryu JH and Cheong JH. Anti-stress effect of Artichoke juice in SD rats and ICR mice, Food Sci Biotech 2004; 13: 302-308.
  26. Ward CP, Rsdd K, Williams BM, Caler JR, Luo Y and Mc- Coy JG. Ginkgo bioloba extract: Cognitive enhancer or anti-stress buffer, Pharmacol Biochem Behav 2002; 72: 913-922. https://doi.org/10.1016/S0091-3057(02)00768-2
  27. Yuan WX, Wu XJ, Yang FX, Shang XH and Zhang LL. Effects of Ginseng root saponins on brain monoamines and serum corticosterone in heat-stressed mice, Zhongguo Yao Li Hsueh Pao Mar 1989; 10: 492-496.