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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Characterization of a novel methionine sulfoxide reductase A from tomato (Solanum lycopersicum), and its protecting role in Escherichia coli

  • Dai, Changbo (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Singh, Naresh Kumar (Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University) ;
  • Park, Myung-Ho (Department of Mechanical Engineering, Kangwon National University)
  • Received : 2011.06.08
  • Accepted : 2011.09.03
  • Published : 2011.12.31
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Abstract

Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and $K_m$ and $K_{cat}$ values for MSRA2 were observed as 8.5, $25^{\circ}C$, $352{\pm}25\;{\mu}M$, and $0.066{\pm}0.009\;S^{-1}$, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage.

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References

  1. Brot, N. and Weissbach, H. (1983) Biochemistry and physiological role of methionine sulfoxide residues in proteins. Arch. Biochem. Biophys. 223, 271-281. https://doi.org/10.1016/0003-9861(83)90592-1
  2. Moskovitz, J., Bar-Noy, S., Williams, W. M., Requena, J., Berlett, B. S. and Stadtman, E. R. (2001) Methionine sulfoxide reductase (MsrA) is a regulator of antioxidant defense and lifespan in mammals. Proc. Natl. Acad. Sci. U.S.A. 98, 12920-12925. https://doi.org/10.1073/pnas.231472998
  3. Moskovitz, J. (2005) Methionine sulfoxide reductases: ubiquitous enzymes involved in antioxidant defense, protein regulation, and prevention of aging-associated diseases. Biochim. Biophys. Acta. 1703, 213-219. https://doi.org/10.1016/j.bbapap.2004.09.003
  4. Lee, B. C., Dikiy, A., Kim, H. Y. and Gladyshev, V. N. (2009) Functions and evolution of selenoprotein methionine sulfoxide reductases. Biochim. Biophys. Acta. 1790, 1471-1477. https://doi.org/10.1016/j.bbagen.2009.04.014
  5. Tarrago, L., Laugier, E. and Rey P. (2009) Protein-Repairing methionine sulfoxide reductases in photosynthetic organisms: gene organization, reduction mechanisms, and physiological roles. Mol. Plant. 2, 202-217. https://doi.org/10.1093/mp/ssn067
  6. Guo, X., Wu, Y., Wang, Y., Chen Y. and Chu, C. (2009) OsMSRA4.1 and OsMSRB1.1, two rice plastidial methionine sulfoxide reductases, are involved in abiotic stress responses. Planta. 230, 227-238. https://doi.org/10.1007/s00425-009-0934-2
  7. Bechtold, U., Murphy, D. J. and Mullineaux, P. M. (2004) Arabidopsis peptide methionine sulfoxide reductase2 prevents cellular oxidative damage in long nights. Plant Cell 16, 908-919. https://doi.org/10.1105/tpc.015818
  8. Cordes, S., Deikman, J., Margossian, L. J. and Fischer, R. L. (1989) Interaction of a developmentally regulated DNA-binding factor with sites flanking two different fruit-ripening genes from tomato. Plant Cell 1, 1025-1034. https://doi.org/10.1105/tpc.1.10.1025
  9. Rouhier, N., Kauffmann, B., Tete-Favier, F., Palladino, P., Gans, P., Branlant, G., Jacquot, J. P. and Boschi-Muller, S. (2007) Functional and structural aspects of poplar cytosolic and plastidial type a methionine sulfoxide reductases. J. Biol. Chem. 282, 3367-3378. https://doi.org/10.1074/jbc.M605007200
  10. Moskovitz, J., Rahman, M. A., Strassman, J., Yancey, S. O., Kushner, S. R., Brot, N. and Weissbach, H. (1995) Escherichia coli peptide methionine sulfoxide reductase gene: regulation of expression and role in protecting against oxidative damage. J. Bacteriol. 177, 502-507. https://doi.org/10.1128/jb.177.3.502-507.1995
  11. Lowther, W. T., Brot, N., Weissbach, H., Honek, J. F. and Matthews, B. W. (2000) Thiol-disulfide exchange is involved in the catalytic mechanism of peptide methionine sulfoxide reductase. Proc. Natl. Acad. Sci. U.S.A. 97, 6463-6468. https://doi.org/10.1073/pnas.97.12.6463
  12. Boschi-Muller, S., Azza S., Sanglier-Cianferani, S., Talfournier, F., Van Dorsselear, A. and Branlant, G. (2000) A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli. J. Biol. Chem. 275, 35908-35913. https://doi.org/10.1074/jbc.M006137200
  13. Vieira Dos Santos, C., Cuine, S., Rouhier, N. and Rey, P. (2005) The Arabidopsis plastidic methionine sulfoxide reductase B proteins. Sequence and activity characteristics, comparison of the expression with plastidic methionine sulfoxide reductase A, and induction by photooxidative stress. Plant Physiol. 138, 909-922. https://doi.org/10.1104/pp.105.062430
  14. Lee, B. C., Lee, Y. K., Lee, H. J., Stadtman, E. R., Lee, K. H. and Chung, N. (2005) Cloning and characterization of antioxidant enzyme methionine sulfoxide-S-reductase from Caenorhabditis elegans. Arch. Biochem. Biophys. 434, 275-281. https://doi.org/10.1016/j.abb.2004.11.012
  15. Davies, M. J. (2005) The oxidative environment and protein damage. Biochim. Biophys. Acta. 1703, 93-109. https://doi.org/10.1016/j.bbapap.2004.08.007
  16. Lopez, A. P., Portales, R. B., López-Ráez, J. A., Medina- Escobar, J. A., Blanco, J. M. and Franco, A. R. (2006) Characterization of a strawberry late-expressed and fruit-specific peptide methionine sulphoxide reductase. Physiol. Plantarum. 1266, 129-139.
  17. Dai, C. and Wang, M. -H. (2010) Expression pattern of a peptide methionine sulfoxide reductase gene from tomato (Solanum lycopersicum) in response to abiotic and oxidative stresses. J. Korean Soc. Appl. Biol. Chem. 53, 127-132. https://doi.org/10.3839/jksabc.2010.022
  18. Minetti, G., Balduini, C. and Brovelli, A. (1994) Reduction of DABS-L-methionine-dl-sulfoxide by protein methionine sulfoxide reductase from polymorphonuclear leukocytes: stereospecificity towards the l-sulfoxide. Ital. J. Biochem. 43, 273-283.

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