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선학초 추출물의 미백활성

Whitening Activities of the Agrimonia pilosa L. Extracts

  • 김동희 ((재)대구경북한방산업진흥원) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 이진영 (호서대학교 한방화장품과학과)
  • Kim, Dong-Hee (Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
  • 투고 : 2011.10.13
  • 심사 : 2011.11.02
  • 발행 : 2011.12.31

초록

선학초 추출물을 기능성 화장품 소재로 활용하기 위하여 미백효과를 검증하였다. 세포 내 tyrosinase 저해활성 측정결과 선학초 에탄올 추출물 $500{\mu}g/mL$에서 42%의 저해활성을 나타내었다. 이는 선학초 추출물이 세포 내 tyrosinase 발현을 억제시킴으로서 멜라닌 합성 또한 저해 하는 것임을 확인 할 수 있었다. 선학초 추출물의 단백질 발현과 mRNA 발현 억제효과를 검토한 결과 선학초 열수 및 에탄올 추출물을 처리한 B16F10군에서는 tyrosinase protein의 발현이 처리하지 않은 군보다 감소함을 확인할 수 있었다. 결과적으로 선학초 추출물의 미백효능을 확인할 수 있었으며, 식품 및 화장품의 기능성 소재로 이용이 가능할 것으로 판단된다.

The extracts of Agrimonia pilosa L. were investigated for the inhibitory effect on the melanin synthesis in B16/F10 mouse melanoma cells as a functional ingredient for cosmetic products. Tyrosinase inhibition activities were 42% in A. pilosa L. 70% ethanol extract at $500{\mu}g/mL$. The protein and mRNA expression of tyrosinase, which are all skin-whitening related factors, showed that A. pilosa L. water and A. pilosa L. 70% ethanol extracts inhibited the protein bio-synthesis in B16F10 melanoma cell. Results indicate that the A. pilosa L. extracts tested in the present study have skin whitening activity and can be used as a functional ingredient for cosmetic compositions.

키워드

참고문헌

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피인용 문헌

  1. Inhibitory effect of melanin synthesis using organic solvent extracts from Scolopendra subspinipes mutilans vol.52, pp.1, 2014, https://doi.org/10.7852/jses.2014.52.1.1
  2. Leaf Extract on the UV-Induced Photoaging-Related Ion Channel, ORAI1, and the Enzymes Tyrosinase and Elastase vol.40, pp.1, 2015, https://doi.org/10.1111/jfbc.12171
  3. Triterpenoids fromFragaria ananassacalyx and their inhibitory effects on melanogenesis in B16-F10 mouse melanoma cells vol.27, pp.23, 2011, https://doi.org/10.1080/14786419.2013.805330
  4. 선이질풀 추출물의 멜라닌 생성 억제 효과 vol.48, pp.1, 2011, https://doi.org/10.9721/kjfst.2016.48.1.72
  5. Prediction of Optimal Microwave Extraction Conditions for Functional Compounds from Agrimonia pilosa Ledeb Using Response Surface Methodology vol.24, pp.4, 2011, https://doi.org/10.7783/kjmcs.2016.24.4.263
  6. B16F10 멜라닌 세포에서 약콩(Glycine soja Siebold et Zucc.) 분획 추출물의 멜라닌 생성 저해 효과 vol.43, pp.3, 2011, https://doi.org/10.15230/scsk.2017.43.3.231
  7. Trends in the Efficacy and Safety of Ingredients in Acne Skin Treatments vol.16, pp.3, 2018, https://doi.org/10.20402/ajbc.2017.0199
  8. 오크라 추출물의 멜라닌 생성저해 및 피부장벽개선 효과 vol.50, pp.3, 2011, https://doi.org/10.9721/kjfst.2018.50.3.344
  9. 말오줌나무 추출물의 미백활성 검증 vol.29, pp.3, 2011, https://doi.org/10.5352/jls.2019.29.3.279