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Quality Characteristics of Brown Rice Vinegar Prepared using Varying Amounts of Nuruk (an Amylolytic Enzyme Preparation) and Employing Different Fermentation Conditions

누룩첨가량 및 배양방법을 달리한 현미식초의 품질특성

  • Lee, Su-Won (Department of Food Science and Technology, Kyungpook National University) ;
  • Kwon, Joong-Ho (Department of Food Science and Technology, Kyungpook National University) ;
  • Yoon, Sung-Ran (Department of Food Science and Technology, Kyungpook National University) ;
  • Woo, Seung-Mi (Department of Food Science and Technology, Keimyung University and Keimyung Foodex Co, Ltd.) ;
  • Yeo, Soo-Hwan (Fermentation and Food Processing Division, Department of Agrofood Resources, NAAS, RDA) ;
  • Jeong, Yong-Jin (Department of Food Science and Technology, Keimyung University and Keimyung Foodex Co, Ltd.)
  • 이수원 (경북대학교 식품공학과) ;
  • 권중호 (경북대학교 식품공학과) ;
  • 윤성란 (경북대학교 식품공학과) ;
  • 우승미 (계명대학교 식품가공학과 및 (주)계명푸덱스) ;
  • 여수환 (농촌진흥청 국립농업과학원 농식품자원부 발효이용과) ;
  • 정용진 (계명대학교 식품가공학과 및 (주)계명푸덱스)
  • Received : 2010.08.04
  • Accepted : 2010.12.24
  • Published : 2011.02.28

Abstract

We added Nuruk at various proportions to brown rice Takju, and investigated the quality characteristics of vinegar produced in agitated culture and static culture. The more Nuruk was added, the higher the alcohol concentration. However, when over 30% (w/w) Nuruk was present, the increment in alcohol content was low (maximum 13.5%, v/v). A comparison of the quality of brown rice vinegar produced in agitated culture with that of vinegar produced in static culture showed that titratable acidity in agitated culture vinegar was highest after fermentation with 30% (w/v) Nuruk, at 5.97%. In static culture, the greater the amount of Nuruk added after 16 days of fermentation, the higher the titratable acidity of vinegar produced; this was true upon addition of either 30% (w/v) or 40% (w/v) Nuruk. Free amino acid levels increased in both agitated and static cultures as the level of added Nuruk rose. Moreover, brown rice vinegar produced in static culture had a higher level (7-30%) of total free amino acids than did vinegar produced in agitated culture. In particular, the level of gamma-amino butyric acid, a functional fortifier, was 3-5-fold higher in vinegar produced in agitated culture. The results thus indicate that both the amount of added Nuruk and the type of fermentation affected the level of free amino acid production. A static culture is expected to undergo changes in aroma and sensory characteristics during fermentation, indicating that further research on vinegars is required.

본 연구에서는 누룩 첨가량을 달리하여 현미탁주를 제조 하고 이를 이용한 현미식초[교반배양(A) 및 정치배양(S)]의 품질 특성을 조사하였다. 누룩 첨가량에 따른 알코올 함량 은 누룩 첨가량이 많을수록 높게 나타났으며 누룩 30% (w/w) 이상 첨가구에서는 약 13.5%로 큰 차이가 없었다. 이를 이용한 교반 및 정치배양 현미식초의 품질특성을 비교한 결과, 교반배양(A)에서 적정산도는 발효 종료 후 CVA에서 5.97%로 가장 높았으며, 정치배양(S)에서는 발효 16일째이후 누룩 첨가량이 많은 순으로 적정산도가 높게 나타났으며, CVS와 DVS는 큰 차이가 없는 것으로 나타났다. 현미 식초의 유리아미노산 함량을 비교한 결과, 교반 및 정치배양에서 총 유리아미노산함량은 누룩 첨가량이 많을수록 증가하였으며, 정치배양 현미식초가 교반배양 현미식초에 비하여 7~30 mg%정도 더 높았다. 특히, 현미의 주요한 기능성 강화물질로 알려진 ${\gamma}$-aminobutyric acid는 교반배양 현미식초보다 정치배양 현미식초에서 약 3~5배 더 높은 것으로 나타났다. 따라서 본 연구에서는 누룩 첨가량 및 초산발효 배양방법에 따라서 유리아미노산 함량 차이가 큰 것으로 판단되며, 정치배양의 경우 향후 숙성과정에서 변화를 거치면서 향기성분, 관능적 특성에 차이가 있을 것으로 예상되어 다양한 식초의 품질변화에 관한 연구가 요구된다.

Keywords

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