DOI QR코드

DOI QR Code

The Inhibitory Effect of Picrasmae Lignum on Inflammatory Responses

고목(苦木)의 염증 반응 억제 효과

  • Ryu, Ik-Han (Dept. of Oriental Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of Oriental Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Song-Baeg (Dept. of Oriental Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Seo, Yun-Jung (Dept. of Oriental Gynecology, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Chang-Min (Dept. of Oriental Gynecology, College of Oriental Medicine, Won-Kwang University)
  • 류익한 (원광대학교 한의과대학 부인과 교실) ;
  • 조한백 (원광대학교 한의과대학 부인과 교실) ;
  • 김송백 (원광대학교 한의과대학 부인과 교실) ;
  • 서윤정 (원광대학교 한의과대학 부인과 교실) ;
  • 최창민 (원광대학교 한의과대학 부인과 교실)
  • Received : 2011.01.28
  • Accepted : 2011.02.09
  • Published : 2011.02.28

Abstract

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Picrasmae Lignum(PL). Methods: To evaluate the anti-inflammatory effects of PL extract, the productions of NO, PGE2 and expression of pro-inflammatory cytokine(IL-1b, IL-6 and TNF-a) were measured in LPS-induced RAW 264.7 cells. Furthermore Western blot analysis has been done to look into the inhibitory mechanisms such as MAPKs and NF-kB. Results: PL extract down-regulated LPS-induced NO, PGE2, IL-1b, IL-6 and TNF-a productions mainly through JNK and p38 MAPK pathway and NF-kB pathway. Conclusions: These results suggest that PL extract may be effective for the treatment of inflammatory diseases.

Keywords

References

  1. Abbas AK, Lichtman AH. Basic Immunology: Functions and Disorders of the Immune System. 2nd ed. Seoul :Panmun Books. 2005:1-39, 263-89.
  2. 대한 병리학회. 병리학 5판. 서울:고문사. 2003:67-94.
  3. 송계용, 지제근, 함의근. 핵심 병리학. 서울:고려의학. 1998:63-4.
  4. 고려대학교 의과대학 병리학교실. 병리학, 서울:신광출판사. 2001:28-31.
  5. 한방여성의학편찬위원회. 한방여성의학 I. 서울:정담. 2007:282-3.
  6. Lefkowith JB. Cycloxygenase-2 specificity and its clinical implications. American Journal of Medicine. 1999;106:43-50. https://doi.org/10.1016/S0002-9343(99)00003-0
  7. 전국한의과대학 공동교재편찬위원회. 본초학. 서울:영림사. 2007:718.
  8. 김창민 등 역. 중약대사전. 서울:정담.1998;1:269-70.
  9. Luyengi L, Suh N, Fong HH. A lignan and four terpenoids from Brucea javanica that induce differentiation with cultured HL-60 promyelocytic leukemia cells. Phytochemistry. 1996;43:409-12. https://doi.org/10.1016/0031-9422(96)00258-0
  10. Nayfeh AH. Introduction to perturbed techniques. New York:John Weiley & Sons. 1981:365-75.
  11. 김영선등. 소태나무(Picrasma quassioides (D.Don) Benn.)의 성분분석과 생리활 성 평가. 생약학회지. 2006;37(1):37-41.
  12. 이정주. Cytotoxic Alkaloids of Picrasma quassioides. 우석대학교 대학원. 2008.
  13. Y Yu, Wang MH. Antioxidant and Anticancer Activity of Fractions from Picrasma quassioides (D. Don) Benn. Methanolic Extract. Korean J Medicinal Crop Sci. 2007;15(5):329-34.
  14. 김유숙. 소태나무 껍질 추출물이 구강암 세포주의 증식억제에 미치는 영향. 조대학교 보건대학원 석사학위논문. 2010.
  15. Lundberg IE. The role of cytokines, chemokines and adhesion molecules in the pathogenesis of idiopathic inflammatory myopathies. Current Rheumatology Report. 2000;2:216-24. https://doi.org/10.1007/s11926-000-0082-y
  16. 이연아 등. 황련 추출물의 항염 효과.J Kyung Hee Univ Med Cent. 2007 ;23(1):21-6.
  17. 염정호. 니켈 및 코발트의 세포독성기전에서 NO의 역할. 대한 산업의학회지. 2001;13(3):274-7.
  18. Matsukawa A et al. Production of IL-1 and IL-1 receptor antagonist and the pathological significance in lipopolysaccharide induced arthritis. Clin Exp Immunol. 1993;93(2):206-11.
  19. Nathan C, Xie QW. Regulation of biosynthesis of nitric oxide. J Biol Chem. 1994;269:13725-8.
  20. Nathan C, Xie QW. Nitric oxide synthases: roles, tolls and controls. Cell. 1994;78:915-8. https://doi.org/10.1016/0092-8674(94)90266-6
  21. Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, patho-physiology, and pharmacology. Pharmacological Reviews. 1991;43(2):109-42.
  22. Miyasaka N, Hirata Y. Nitric oxide and inflammatory arthritides. Life Sci. 1997;61(21):2073-81. https://doi.org/10.1016/S0024-3205(97)00585-7
  23. 전국의과대학교수. 생리학. 서울:한우리. 1999:640.
  24. Simmons ML, Murphy S. Induction of nitric oxide from glial cells. J Neurochem. 1992;59:897-905. https://doi.org/10.1111/j.1471-4159.1992.tb08328.x
  25. 서영준. 발암과정에있어서Cyclooxygenase-2 의 역할 및 그 저해를 통한 화학 암예방. 분자세포생물학뉴스. 2001;13(14) :8-17.
  26. Johanne MP, Pelletier JP, Fahmi H. Cyclooxygenase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum. 2003;33(3):155-67. https://doi.org/10.1016/S0049-0172(03)00134-3
  27. Needleman P, Isakson PC. The discovery and function of COX-2. J Rheumatol Suppl. 1997;49:6-8.
  28. Seybold VS, Jia YP, Abrahams LG. Cyclo-oxygenase-2 contributes to central sensitization in rats with perepheral inflammation. Pain. 2003;105:47-55. https://doi.org/10.1016/S0304-3959(03)00254-9
  29. 강재성 등. 세포분자면역학 4판. 서울:범문사. 2002:4-5, 240-53, 276-9.
  30. 서울대학교 의과대학. 면역학. 서울: 서울대학교출판부. 1997:123-6.
  31. 권명상 등. 임상면역학. 서울:고려의학. 1998:163-6, 172.
  32. 정민교. 중이염의 발병기전에 있어 염 증매개체들의 역할. Korean J Otolaryngol. 2007;50:2-13.
  33. Pennica D et al. Human tumour necrosis factor: Precursor structure, expression and homology to lymphotoxin. Nature. 1984;312:724-9. https://doi.org/10.1038/312724a0
  34. Abbas AK, Lichtman AH, Pober JS. Cellular and Molecular Immunology. 3rd ed. philadelphia:W.B.Saunders company. 1997:11, 25-6, 250-64, 397.
  35. 주진덕. 마취통증의학과 영역에서 Intra -cellular Signaling Pathway의 이용. Korean J Anesthesiol. 2009;57(3) :277-83. https://doi.org/10.4097/kjae.2009.57.3.277
  36. Means TK, Golenbock DT, Fenton MJ. The biology of Toll-like receptors. Cytokine Growth Factor Rev. 2000; 11:219-32. https://doi.org/10.1016/S1359-6101(00)00006-X
  37. 김종흔 등. 백서 해마에서 카이닌산 유도 경련에 의한 JNK 경유 신호전달경로 활성화의 발달단계에 따른 변화. 대한 신경 정신 의학회. 2001; 40(5):971-80.
  38. 송현. 세포 이동성에 있어서 MAPK 신호 전달경로의 조절기관 규명. 덕 성여자대학교 대학원 박사학위논문. 2006.
  39. 임병우, 최동국, 석경호. 기초면역학. 서울:효일. 2006:201-11.
  40. Garrington TP, Johnson GL. Organization and regulation of mitogen-activated protein kinase signaling pathways. Curr Opin Cell Biol. 1999;11:211-8. https://doi.org/10.1016/S0955-0674(99)80028-3
  41. Lee AK et al. Inhibition of lipopolysaccharide-inducible nitiric oxide synthase. TNF$\alpha$ and COX-2 expression by suchinone effects on I-${\kappa}B{\alpha}$ phosphorylation. C/EBP and AP-1 activation. British J Phamacol. 2003;139:11-20.
  42. 이명식, 김경아. 내분비-대사질환에서 NF-${\kappa}B$의 최신 지견. 대한내분비학회지. 2006;21(5):352-63.
  43. Kaltschmidt B, Sparna T, Kaltschmidt C. Activation of NF-kappa B by reactive oxygen intermediates in the nervous system. Antioxid Redox Signal. 1999;1:129-44. https://doi.org/10.1089/ars.1999.1.2-129
  44. Straus DS et al. 5-deoxy-delta 12,14 -prostaglandin J2 inhibits multiple steps in the NF-kappa B signaling pathway. Proc Natl Acad Sci USA 97. 2000:4844-9.