The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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The anti-inflammatory effects of Cicadidae Periostracum

선태(蟬蛻)의 항염효과(抗炎效果)에 대한 실험적(實驗的) 연구(硏究)

  • Kim, Kyoung-Wan (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Kim, Song-Baeg (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Choe, Chang-Min (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Seo, Yun-Jung (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
  • 김경완 (원광대학교 한의과대학 부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 부인과학교실) ;
  • 김송백 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실) ;
  • 서윤정 (원광대학교 한의과대학 부인과학교실)
  • Received : 2011.01.31
  • Accepted : 2011.02.09
  • Published : 2011.02.28
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Abstract

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Cicadidae Periostracum(CP) on the RAW 264.7 cells. Method: We examined the cytokine productions including nitric oxide(NO), interleukin(IL)-1b, IL-6 and tumor necrosis factor-a(TNF-a) in lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms such as mitogen -activated protein kinases(MAPKs) and nuclear factor kappa BNF-kB) using Western blot. Results: CP inhibited LPS-induced production of NO, IL-1b and TNF-a but not of IL-6 in RAW 264.7 cells. CP respectively inhibited the activation of MAPKs such as extracelluar signal-regulated kinase(ERK 1/2), c-Jun $NH_2$-terminal kinase(JNK), p38 and NF-kB in the LPS-stimulated RAW 264.7 cells. Also oral administration of CP inhibited CLP - induced endotoxin shock. Conclusion: our results showed that CP down-regulated LPS-induced NO, IL-1b and TNF-a productions mainly through ERK, JNK, p38 MAPK and NF-kB pathway, which suggest the anti-inflammatory effects of CP.

Keywords

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