Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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DNA Binding Specificity of Proteus mirabilis Transcription Regulator

Proteus mirabilis 전사 조절 단백질의 DNA 결합 특성

  • Received : 2011.04.08
  • Accepted : 2011.05.26
  • Published : 2011.06.30
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Abstract

Amino acid sequence alignment shows that $\underline{P}$roteus $\underline{m}$irabilis $\underline{t}$ranscription $\underline{r}$egulator (PMTR) has cystein sequence homology at metal binding domain to CueR (copper resistance) protein, which conserves two cysteins (Cys 112 and Cys 120 in PMTR). Gel shift assay revealed that PMTR protein bound to promoter region of Escherichia coli copA (copper-translocating P-type ATPase) and Proteus mirabilis atpase (putative copper-translocating P-type ATPase) genes except that of E. coli zntA (zinc-translocating P-type ATPase) gene. DNase I protection experiment indicated that PMTR protein protected the region over -35 box and close to -10 box. DNase I hypersensitive bases were shown at C and A bases of labeled template strand and at G and C bases of labeled non-template strand of DNA. These hypersensitive bases were appeared in other metalloregulatory proteins of MerR family, which suggests protein-induced DNA bending.

Proteus mirabilis 전사 조절($\underline{P}$roteus $\underline{m}$irabilis $\underline{t}$ranscription $\underline{r}$egulator ) 단백질의 중금속 결합 부위에 대한 아미노산 서열분석에서 PMTR 단백질은 ZntR (아연 저항성) 단백질이 아닌 CueR (구리 저항성) 단백질과 동일한 환경이다. 그리고 겔시프트 법(gel shift assay) 실험에 의하면 PMTR 단백질은 Escherichia coli의 zntA (zinc-translocating P-type ATPase gene) 프로모터에 결합하지 않고 copA (copper-translocating P-type ATPase gene) 프로모터와 Proteus mirabilis에서 atpase (copper-translocating P-type ATPase gene) 프로모터에 결합하였다. DNase I protection 실험에서 PMTR 단백질 결합부위와 DNase I 민감성 염기들이 관찰되었다. P. mirabilis atpase 프로모터에서 민감성 염기로 주형가닥(template strand)에서 C와 A 그리고 비주형가닥(non-template strand)에서 G와 C 염기들이다. 이런 민감성 염기들은 다른 MerR 패밀리 단백질에서 또한 관찰되었으며, 이것은 단백질에 의한 DNA bending을 의미한다.

Keywords

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